Role of laser-induced plasma formation in pulsed cellular microsurgery and micromanipulation

We investigate experimentally the physical processes underlying pulsed cellular microsurgery and micromanipulation using nanosecond 532- and 1064-nm laser pulses focused at high numerical aperture. We find that the laser parameters employed for many microirradiation techniques are congruent with those leading to optical breakdown in water. We determine the size and shape of the laser-induced plasma, pressure of the emitted shock wave, and size and energy of the cavitation bubble formed by the expanding plasma. We discuss implications of the results for biophysical microirradiation procedures.     

Attomole quantitation of protein separations with accelerator mass spectrometry

Quantification of specific proteins depends on separation by chromatography or electrophoresis followed by chemical detection schemes such as staining and fluorophore adhesion. Chemical exchange of short-lived isotopes, particularly sulfur, is also prevalent despite the inconveniences of counting radioactivity. Physical methods based on isotopic and elemental analyses offer highly sensitive protein quantitation that has linear response over wide dynamic ranges and is independent of protein conformation. Accelerator mass spectrometry quantifies long-lived isotopes such as 14C to subattomole sensitivity. We quantified protein interactions with small molecules such as toxins, vitamins, and natural biochemicals at precisions of 1-5%. Micro-proton-induced X-ray emission quantifies elemental abundances in separated metalloprotein samples to nanogram amounts and is capable of quantifying phopsphorylated loci in gels. Accelerator-based quantitation is a possible tool for quantifying the genome translation into proteome.     

Ein starker Normalisationssatz für die bar-rekursiven Funktionale

Ohne Zusammenfassung     

The coordination of Ni11 in glasses and glass-ceramics of the system MgOAl2O3SiO2

Differences in strength connected with colour variations in NiO-containing Mg-alumosilicate glass ceramics give rise to a remission spectroscopic analysis of the Ni-on. In the base glass, Ni¹¹ occupies tetrahedral and octahedral positions of the dark green NiAl spinel. An increase of the Mg-component and a rise of the melting intensity promote the octahedral nickel coordination and along with it the formation of the strength-reducing, light green Ni-forsterite. The demand for a strong final product is thus met by the technologically desirable shortening of the melting time.     

Lineare Programme und allgemeine Vertretersysteme

Ohne Zusammenfassung     

A multidirectional Dirichlet problem

B.E.J. Dahlberg’s theorems on the mutual absolute continuity of harmonic and surface measures, and on the unique solvability of the Dirichlet problem for Laplace’s equation with data taken in L^p spaces p > 2 ? δ are extended to compact polyhedral domains of ?ⁿ. Consequently, for q < 2 + δ, Dahlberg’s reverse Hölder inequality for the density of harmonic measure is established for compact polyhedra that additionally satisfy the Harnack chain condition. It is proved that a compact polyhedral domain satisfies the Harnack chain condition if its boundary is a topological manifold. The double suspension of the Mazur manifold is invoked to indicate that perhaps such a polyhedron need not itself be a manifold with boundary; see the footnote in Section 9. A theorem on approximating compact polyhedra by Lipschitz domains in a certain weak sense is proved, along with other geometric lemmas.