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51.
Cortez E Stumbo AC Saldanha-Gama R Villela CG Barja-Fidalgo C Rodrigues CA das Graças Henriques M Benchimol M Barbosa HS Porto LC Carvalho L 《Micron (Oxford, England : 1993)》2008,39(1):25-31
Toxoplasma gondii is an apicomplexan parasite infecting a broad host range, including humans. The parasite invades host cell by active penetration with the participation of its secretory organelles proteins during this process. Until now, only a limited number of secretory proteins have been discovered, and the effectors molecules involved in parasite invasion and survival are not well understood. Osteopontin (OPN) is a multifunctional glycophosphoprotein, secreted by different cell types, which is involved in various physiological and pathological events including cell signaling and survival. For the first time we demonstrated in this work by immunofluorescence and immunoelectron microscopy approaches the localization of an OPN-like protein in dense granules of extracellular T. gondii tachyzoites. Western blotting and RT-PCR confirmed this protein expression by the parasites. Our results also showed, after macrophage invasion, an intense positive labeling for OPN-like protein at the sub-apical portion of tachyzoites, the site of dense granules secretion, and the localization of this protein at the parasitophorous vacuole membrane. These data suggest that dense granules secrete an OPN-like protein, and we speculate that this protein participates during the parasite interaction process with host cells and parasitophorous vacuole formation. 相似文献
52.
Direct Synthesis of Hexagonal NaGdF4 Nanocrystals from a Single‐Source Precursor: Upconverting NaGdF4:Yb3+,Tm3+ and Its Composites with TiO2 for Near‐IR‐Driven Photocatalysis 下载免费PDF全文
Ying Chen Dr. Shashank Mishra Dr. Gilles Ledoux Dr. Erwann Jeanneau Marlene Daniel Prof. Jinlong Zhang Prof. Stéphane Daniele 《化学:亚洲杂志》2014,9(9):2415-2421
A novel single‐source precursor NaGd(TFA)4(diglyme) (TFA=trifluoroacetate) was synthesized, characterized thoroughly, and used to obtain the hexagonal phase of NaGdF4 nanoparticles as an efficient matrix for lanthanide‐doped upconverting nanocrystals (NCs) that convert near‐infrared radiation into shorter‐wavelength UV/visible light. These NCs were then used to prepare well‐characterized TiO2@NaGdF4:Yb3+,Tm3+ nanocomposites to extend the absorption range of the TiO2 photocatalyst from the UV to the IR region. While the visible/near IR part of the photoluminescent spectra remains almost unaffected by the presence of TiO2, the UV part is strongly quenched due to the absorption of TiO2 above its gap at approximately 380 nm by energy transfer or FRET. Preliminary results on the photocatalytic activity of the above obtained nanocomposites are presented. 相似文献
53.
Maximum Spin Polarization in Chromium Dimer Cations as Demonstrated by X‐ray Magnetic Circular Dichroism Spectroscopy 下载免费PDF全文
Dr. Vicente Zamudio‐Bayer Dr. Konstantin Hirsch Dr. Andreas Langenberg Dipl.‐Phys. Markus Niemeyer Dr. Marlene Vogel Dr. Arkadiusz Ławicki Prof. Dr. Akira Terasaki Dr. J. Tobias Lau Prof. Dr. Bernd von Issendorff 《Angewandte Chemie (International ed. in English)》2015,54(15):4498-4501
X‐ray magnetic circular dichroism spectroscopy has been used to characterize the electronic structure and magnetic moment of Cr2+. Our results indicate that the removal of a single electron from the 4sσg bonding orbital of Cr2 drastically changes the preferred coupling of the 3d electronic spins. While the neutral molecule has a zero‐spin ground state with a very short bond length, the molecular cation exhibits a ferromagnetically coupled ground state with the highest possible spin of S=11/2, and almost twice the bond length of the neutral molecule. This spin configuration can be interpreted as a result of indirect exchange coupling between the 3d electrons of the two atoms that is mediated by the single 4s electron through a strong intraatomic 3d‐4s exchange interaction. Our finding allows an estimate of the relative energies of two states that are often discussed as ground‐state candidates, the ferromagnetically coupled 12Σ and the low‐spin 2Σ state. 相似文献
54.
55.
Improvement of microbial cell cultures oxygenation can be achieved by the increase of total air pressure, which increases
oxygen solubility in the medium. In this work, a pressurized bioreactor was used for Yarrowia lipolytica batch cultivation under increased air pressure from 1 to 6 bar. Cell growth was strongly enhanced by the pressure rise. Fivefold
and 3.4-fold increases in the biomass production and in specific growth rate, respectively, were observed under 6 bar. The
increase of oxygen availability caused the induction of the antioxidant enzyme superoxide dismutase, which indicates that
the defensive mechanisms of the cells against oxidative stress were effective and cells could cope with increased pressure.
The pregrowth of Y. lipolytica under increased pressure conditions did not affect the lipase production ability of the cells. Moreover, the extracellular
lipase activity increased 96% using a 5-bar air pressure instead of air at 1-bar pressure during the enzyme production phase.
Thus, air pressure increase in bioreactors is an effective mean of cell mass and enzyme productivity enhancement in bioprocess
based in Y. lipolytica cultures. 相似文献
56.
Marlene Espinoza-Moraga 《Tetrahedron letters》2009,50(50):7059-7061
An alternative synthetic approach for the introduction of chirality in β-carboline moiety through in situ reduction of N-acyliminium ion intermediates generated from imine 2 and chloroformate of 8-phenylmenthyl as chiral auxiliary was achieved. The method applied microwave-assisted irradiation and used PdCl2/Et3SiH protocol as a mild reducing agent, which decreased reaction times to minutes when compared to the conventional thermal reactions. The diastereoselectivity (4-12:1) of the reduction produced R-amines, which were assigned after chiral auxiliary removal and spectroscopic data compared to products obtained from Noyori asymmetric hydrogenation catalyst. 相似文献
57.
Fraschetti C Letzel MC Paletta M Mattay J Speranza M Filippi A Aschi M Rozhenko AB 《Journal of mass spectrometry : JMS》2012,47(1):72-78
The effect of cyclochirality of rccc-2,8,14,20-tetra-n-decyl-4,10,16,22-tetra-O-methylresorcin[4]arene (C) on the enantiodiscrimination of a number of chiral bidentate and tridentate aromatic and aliphatic biomolecules (G) has been investigated by nano-electrospray ionization (nano-ESI)-Fourier transform ion cyclotron resonance mass spectrometry. The experimental approach is based on the formation of diastereomeric proton-bound [C·H·G](+) complexes by nano-ESI of solutions containing an equimolar amount of quasi-enantiomers (C) together with the chiral guest (G) and the subsequent measurement of the rate of the G substitution by the attack of several achiral and chiral amines. In general, the heterochiral complexes react faster than the homochiral ones, except when G is an aminoalcoholic neurotransmitter whose complexes, beyond that, exhibit the highest enantioselectivity. The kinetic results were further supported by both collision-induced dissociation experiments on some of the relevant [C(2) ·H·G](+) three-body species and Density functional theory (DFT) calculations performed on the most selective systems. 相似文献
58.
We investigate the relaxational dynamics of the order parameter of chiral symmetry breaking, the sigma mean-field, with a heat bath consisting of quarks and antiquarks. A semiclassical stochastic Langevin equation of motion is obtained from the linear sigma model with constituent quarks. The equilibration of the system is studied for a first order phase transition and a critical point, where a different behavior is found. At the first order phase transition we observe the phase coexistence and at a critical point the phenomenon of critical slowing down with large relaxation times. We go beyond existing Langevin studies and include reheating of the heat bath by determining the energy dissipation during the relaxational process. The energy of the entire system is conserved. In a critical point scenario we again observe critical slowing down. 相似文献
59.
Bile salt aggregates are supramolecular structures with two types of binding sites, called primary and secondary sites. The objective of this work was to explore how the nonplanarity and size of guests (biphenyl [BP], 1-1'-binaphthyl [BNP] and dibenz[b,f]oxepin [DBX]) affected their binding affinity and dynamics to sodium cholate (NaC) aggregates. Fluorescence and laser-flash photolysis experiments were performed to obtain information on the binding environment for the guests, the accessibility of quenchers to guests in the aggregate and the dissociation rate constants of the guests from the aggregates. All guests were bound to the more hydrophobic primary aggregate, showing that this site can accommodate nonplanar molecules. However, the structure of the guest affects the structure of the primary aggregates, leading to changes in the accessibility of anions to aggregate-bound guests and to changes for the guest dissociation rate constants from the aggregates. 相似文献
60.
Brienne Sprague Qian Shi Marlene T. Kim Liying Zhang Alexander Sedykh Eiichiro Ichiishi Harukuni Tokuda Kuo-Hsiung Lee Hao Zhu 《Journal of computer-aided molecular design》2014,28(6):631-646
Compared to the current knowledge on cancer chemotherapeutic agents, only limited information is available on the ability of organic compounds, such as drugs and/or natural products, to prevent or delay the onset of cancer. In order to evaluate chemical chemopreventive potentials and design novel chemopreventive agents with low to no toxicity, we developed predictive computational models for chemopreventive agents in this study. First, we curated a database containing over 400 organic compounds with known chemoprevention activities. Based on this database, various random forest and support vector machine binary classifiers were developed. All of the resulting models were validated by cross validation procedures. Then, the validated models were applied to virtually screen a chemical library containing around 23,000 natural products and derivatives. We selected a list of 148 novel chemopreventive compounds based on the consensus prediction of all validated models. We further analyzed the predicted active compounds by their ease of organic synthesis. Finally, 18 compounds were synthesized and experimentally validated for their chemopreventive activity. The experimental validation results paralleled the cross validation results, demonstrating the utility of the developed models. The predictive models developed in this study can be applied to virtually screen other chemical libraries to identify novel lead compounds for the chemoprevention of cancers. 相似文献