14-Noreudesmanes and a phenylpropane heterodimer from sea buckthorn berry inhibit Herpes simplex type 2 virus replication

Two new 14-noreudesmane sesquiterpenes, one new phenylpropane heterodimer, caulilexin C, and uvaol were isolated from the 70% MeOH extract of the fruit peel of Elaeagnus rhamnoides. The structures of the compounds were elucidated by HRESIMS and advanced NMR methods. The absolute configuration of (R)-6,9-dihydroxy-1-oxo-14-noreudesm-5,7,9-triene was determined by the TDDFT-ECD method. The new compounds, together with structurally similar naphthalenes (musizin, musizin-8-O-glucoside, torachrysone-8-O-glucoside) and 1,4-naphthoquinone (2-methylstipandrone), isolated previously from Rumex aquaticus, were investigated for their antiviral activity against Herpes simplex virus type 2 (HSV-2) using two different methods. Applying the traditional virus yield reduction test, (R)-6,9-dihydroxy-1-oxo-14-noreudesm-5,7,9-triene, 1-[3-methoxy-4-(2-methoxy-4-(1E)-propenyl-phenoxy)-phenyl]-propane-1,2-diol, and musizin caused a 2.00 log₁₀, 3.49 log₁₀, and 2.33 log₁₀ reduction of HSV-2 yield, respectively, at a concentration of 12.5?μM. 2-Hydroxy-1-methoxy-6,9-dioxo-14-noreudesm-1,3,5(10),7-tetraene exhibited an antiviral effect at concentration of 50?μM only. Similar results were obtained when the qPCR method was used to test the antiviral activity of the compounds.     

Capillary zone electrophoresis of lipoarabinomannan by multi-layered concentration

The present article describes a capillary zone electrophoresis method which relies on a multilayered water-alkali solvent stacking with online ionization to enhance detection of mannose, arabinose, and their oligosaccharides, which are used as the migration profile standards but are also the distinctive structural components of lipoarabinomannan. Lipoarabinomannan is detected in patients having tuberculosis. The capillary electrophoresis method with ionization of the whole saccharides without degradation in alkaline solution inside the capillary is based on the structural deprotonation of the molecules under ultrahigh pH conditions. The validation of the capillary electrophoresis parameters revealed that the 15-fold electrolyte–water-injection plug allowed detection of one-third lower concentrations than detected without online concentration. For the first time, the better detectability was seen especially for highly polymerized, but otherwise poorly ionized, arabinooctaose. The applicability of the method for detecting whole large biological saccharide complexes was confirmed by the glycolipid lipoarabinomannan. For the first time also, the migration of the indestructible lipoarabinomannan was detected together with oligosaccharides used representing the capping units, namely mannose, mannobiose, and mannotriose. The myo-inositol-phosphate-lipid unit was seen to migrate separately from the arabinomannan, since it was hydrolyzed from one lipoarabinomannan product under alkaline conditions in capillary electrophoresis.     

Isolation and structure determination of new 4,12-dideoxyphorbol esters from Euphorbia pannonica Host.

Phytochemical study of the aerial parts of Euphorbia pannonica led to the isolation of two new tigliane diterpenes, 4,12-dideoxy(4β)phorbol-20-benzoate-13-isobutyrate (1) and 4,12-dideoxy(4β)phorbol-20-benzoate-13-isovalerianate (2). The structures and relative stereochemistry were elucidated on the basis of extensive spectroscopic analyses, including 1D and 2D NMR experiments (¹H NMR, JMOD, COSY, NOESY, HSQC and HMBC).     

On a Sobolev-type inequality related to the weighted p-Laplace operator

In this paper we deal with some Sobolev-type inequalities with weights that were proved by Maz'ya in [V.G. Maz'ja, Sobolev Spaces, Springer-Verlag, Berlin, 1980] and by Caffarelli, Kohn and Nirenberg in [L. Caffarelli, R. Kohn, L. Nirenberg, First order interpolation inequalities with weight, Compos. Math. 53 (1984) 259-275]. For integers 1?k?N denote points ξ∈RN=Rk×RN−k as pairs (x,y). Let p∈(1,N), q∈(p,p^∗] and assume . Then there exists c>0 such that

     

Fluoro Nitrenoid Complexes FN=MF2 (M=Co,Rh, Ir): Electronic Structure Dichotomy and Formation of Nitrido Fluorides N≡MF3

The fluoronitrenoid metal complexes FNCoF₂ and FNRhF₂ as well as the first ternary Rh^VI and Ir^VI complexes NIrF₃ and NRhF₃ are described. They were obtained by the reaction of excited Group-9 metal atoms with NF₃ and their IR spectra, isolated in solid rare gases (neon and argon), were recorded. Aided by the observed ^14/15N isotope shifts and quantum-chemical predictions, all four stretching fundamentals of the novel complexes were safely assigned. The F−N stretching frequencies of the fluoronitrenoid complexes FNCoF₂ (1056.8 cm⁻¹) and FNRhF₂ (872.6 cm⁻¹) are very different and their N−M bonds vary greatly. In FNCoF₂, the FN ligand is singly bonded to Co and bears considerable iminyl/nitrene radical character, while the N−Rh bond in FNRhF₂ is a strong double bond with comparatively strong σ- and π-bonds. The anticipated rearrangement of FNCoF₂ to the nitrido Co^VI complex is predicted to be endothermic and was not observed.     

Antiproliferative Phenanthrenes from Juncus tenuis: Isolation and Diversity-Oriented Semisynthetic Modification

The occurrence of phenanthrenes is limited in nature, with such compounds identified only in some plant families. Phenanthrenes were described to have a wide range of pharmacological activities, and numerous research programs have targeted semisynthetic derivatives of the phenanthrene skeleton. The aims of this study were the phytochemical investigation of Juncus tenuis, focusing on the isolation of phenanthrenes, and the preparation of semisynthetic derivatives of the isolated compounds. From the methanolic extract of J. tenuis, three phenanthrenes (juncusol, effusol, and 2,7-dihydroxy-1,8-dimethyl-5-vinyl-9,10-dihydrophenanthrene) were isolated. Juncusol and effusol were transformed by hypervalent iodine(III) reagent, using a diversity-oriented approach. Four racemic semisynthetic compounds possessing an alkyl-substituted p-quinol ring (1–4) were produced. Isolation and purification of the compounds were carried out by different chromatographic techniques, and their structures were elucidated by means of 1D and 2D NMR, and HRMS spectroscopic methods. The isolated secondary metabolites and their semisynthetic analogues were tested on seven human tumor cell lines (A2780, A2780cis, KCR, MCF-7, HeLa, HTB-26, and T47D) and on one normal cell line (MRC-5), using the MTT assay. The effusol derivative 3, substituted with two methoxy groups, showed promising antiproliferative activity on MCF-7, T47D, and A2780 cell lines with IC₅₀ values of 5.8, 7.0, and 8.6 µM, respectively.     

A subdivision algorithm for the computation of unstable manifolds and global attractors

Summary. Each invariant set of a given dynamical system is part of the global attractor. Therefore the global attractor contains all the potentially interesting dynamics, and, in particular, it contains every (global) unstable manifold. For this reason it is of interest to have an algorithm which allows to approximate the global attractor numerically. In this article we develop such an algorithm using a subdivision technique. We prove convergence of this method in a very general setting, and, moreover, we describe the qualitative convergence behavior in the presence of a hyperbolic structure. The algorithm can successfully be applied to dynamical systems of moderate dimension, and we illustrate this fact by several numerical examples. Received May 11, 1995 / Revised version received December 6, 1995     

Lifetime measurements of highly excited Rydberg states of strontium I

Lifetimes of Rydberg states of the triplet-series 5s ns ³ S ₁ withn = 19–23, 35 and 5s nd ³ D ₃ withn = 18–20, 23–28 in the spectrum of neutral strontium have been determined. Observation of the exponential decay after excitation by a pulsed laser in a fast atomic beam and subsequent state-selective field ionization was employed. The lifetimes of the states of the³ S ₁-series show the expectedn*³ dependence on the effective principal quantum number, while the³ D ₃-series is disturbed by configuration mixing. Furthermore, state re-populations induced by black-body radiation have been observed.     

Optimization and optimality test for the Max-Cut Problem

We show that the following two problems are polynomially equivalent: