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71.
Takwa M Larsen MW Hult K Martinelle M 《Chemical communications (Cambridge, England)》2011,47(26):7392-7394
Based on molecular modelling, the enzyme Candida antarctica lipase B was redesigned as a catalyst for the ring opening polymerization of D,D-lactide. Two mutants with 90-fold increased activity as compared to the wild-type enzyme were created. In a preparative synthesis of poly(D,D-lactide) the mutants greatly improved the rate and the degree of polymerization. 相似文献
72.
Marianne Akian St��phane Gaubert Bas Lemmens 《Journal of Fixed Point Theory and Applications》2011,9(2):295-325
We study the stable behaviour of discrete dynamical systems where the map is convex and monotone with respect to the standard
positive cone. The notion of tangential stability for fixed points and periodic points is introduced, which is weaker than
Lyapunov stability. Among others we show that the set of tangentially stable fixed points is isomorphic to a convex inf-semilattice,
and a criterion is given for the existence of a unique tangentially stable fixed point. We also show that periods of tangentially
stable periodic points are orders of permutations on n letters, where n is the dimension of the underlying space, and a sufficient condition for global convergence to periodic orbits is presented. 相似文献
73.
74.
Muriel De Bock Marie-Alice Meuwis Tran Quang Minh Jean-Paul Chapelle Michel Malaise Marianne Fillet 《Talanta》2010,82(1):245-594
In most diseases, the clinical need for serum/plasma markers has never been so crucial, not only for diagnosis, but also for the selection of the most efficient therapies, as well as exclusion of ineffective or toxic treatment. Due to the high sample complexity, prefractionation is essential for exploring the deep proteome and finding specific markers.In this study, three different sample preparation methods (i.e., highly abundant protein precipitation, restricted access materials (RAM) combined with IMAC chromatography and peptide ligand affinity beads) were investigated in order to select the best fractionation step for further differential proteomic experiments focusing on the LMW proteome (MW inferior to 40,000 Da). Indeed, the aim was not to cover the entire plasma/serum proteome, but to enrich potentially interesting tissue leakage proteins. These three methods were evaluated on their reproducibility, on the SELDI-TOF-MS peptide/protein peaks generated after fractionation and on the information supplied.The studied methods appeared to give complementary information and presented good reproducibility (below 20%). Peptide ligand affinity beads were found to provide efficient depletion of HMW proteins and peak enrichment in protein/peptide profiles. 相似文献
75.
The thermotropic liquid crystalline behaviour of two N-alkylpyridinium tetrahalocuprates was investigated. The n-pentadecyl tetrachloro and tetrabromo derivatives (CU15-CL and CU15-BR, respectively) exhibit very similar behaviour on cooling, and specifically, a high temperature smectic phase followed by a cubic phase. We fully elucidated the Ia3¯d space group for the cubic phase from X-ray diffraction on single crystals. The type of cubic phase is still under discussion, but we suggest that it is a direct type of structure based on the known phase sequence of lower and higher homologues. A model for the local organization of CU15-CL and CU15-BR in the cubic and smectic mesophases is also proposed. 相似文献
76.
Marianne Wrenger M. Oellerich E. Raude M. Riedmann 《Fresenius' Journal of Analytical Chemistry》1986,324(3-4):350-351
Conclusion The precision and accuracy of the HPLC methods appear to be sufficient for routine drug measurements. They show a good practicability, as mobile phase adjustments are part of the methods and no sample preparation is required. Although homogeneous non-isotopic immunoassays may be preferable for stat analyses, the described HPLC procedure is an attractive alternative for measuring several drugs and metabolites simultaneously in a single specimen. It is to be expected that numerous further drugs (e.g. anticonvulsants, antiarrhythmics) can be measured by this Chromatographic technique under similar conditions.
HPLC-Methoden für das Drug-Monitoring mit direkter Seruminjektion, Säulenschaltung und einfacher Umstellung der mobilen Phasen相似文献
77.
78.
James D. Oliver Adam A. Rosser Christopher M. Fellows Yohann Guillaneuf Jean-Louis Clement Marianne Gaborieau Patrice Castignolles 《Analytica chimica acta》2014
Direct UV detection of carbohydrates in free solution capillary electrophoresis at 270 nm is made possible by a photo-oxidation reaction. Glucose, rhamnose and xylose were shown to have unique UV absorption spectra hypothesizing different UV absorbing intermediates for their respective photo-oxidation. NMR spectroscopy of the photo-oxidation end products proved they consisted of carboxylates and not malondialdehyde as previously theorized and that oxygen thus plays a key role in the photo-oxidation pathway. Adding the photo-initiator Irgacure® 2959 in the background electrolyte increased sensitivity by 40% at an optimum concentration of 1 × 10−4 mM and 1 × 10−8 mM for conventional 50 μm i.d. capillaries and for the corresponding extended light path capillaries, respectively. 相似文献
79.
Bomholt J Moth-Poulsen K Harboe M Karlson AO Qvist KB Bjørnholm T Stamou DG 《Langmuir : the ACS journal of surfaces and colloids》2011,27(3):866-869
The aggregation of casein micelles (CMs) induced by milk-clotting enzymes is a process of fundamental importance in the dairy industry for cheese production; however, it is not well characterized on the nanoscale. Here we enabled the monitoring of the kinetics of aggregation between single CMs (30-600 nm in diameter) by immobilizing them on a glass substrate at low densities and subsequently imaging them with fluorescence microscopy. We validated the new method by a quantitative comparison to ensemble measurements of aggregation. Single-particle statistics allowed us to observe for the first time several heterogeneities in CM aggregation. We observed two types of CM growth: a slow increase in the size of CMs and a stepwise increase attributed to interactions between aggregates preformed in solution. Both types of growth exhibit a lag phase that was very heterogeneous between different CMs, suggesting significant differences in their composition or structure. Detailed size histograms of CMs during aggregation also revealed the presence of two distinct subpopulations with different growth amplitudes and kinetics. The dependence of these distinct nanoscale processes/parameters on aggregation conditions is not accessible to bulk measurements that report only ensemble-average values and may prove important to an in-depth understanding of CM aggregation. 相似文献
80.
Nguyen S Alund SJ Hiorth M Kjøniksen AL Smistad G 《Colloids and surfaces. B, Biointerfaces》2011,88(2):664-673
The present study investigated the surface coating of charged liposomes by three different types of pectin (LM, HM and amidated pectin) by particle size determinations and zeta potential measurements. The pectins and the pectin coated liposomes were visualized by atomic force microscopy. The adsorption of pectin onto positive liposomes yielded a reproducible increase in particle size and a shift of the zeta potential from positive to negative side for all three pectin types, whereas the adsorption of pectin onto negative liposomes did not render any significant changes probably due to electrostatic repulsion. The positive liposomes coated with HM-pectin gave the largest pectin coated particles with the least negative zeta potential, while the opposite was observed for the LM-pectin coated positive liposomes. Furthermore, results from dynamic light scattering revealed narrow size distributions, indicating that the degree of aggregation was low for the pectin coated liposomes. As liposomes are able to encapsulate drugs and pectin has been found to be mucoadhesive, these pectin coated liposomes may be potential drug delivery systems. 相似文献