Analytical theory of gamma-encoded double-quantum recoupling sequences in solid-state nuclear magnetic resonance

Many important double-quantum recoupling techniques in solid-state NMR are classified as being gamma-encoded. This means that the phase of the double-quantum effective Hamiltonian, but not its amplitude, depends on the third Euler angle defining the orientation of the molecular spin system in the frame of the magic-angle-spinning rotor. In this paper, we provide closed analytical solutions for the dependence of the powder-average double-quantum-filtered signal on the recoupling times, within the average Hamiltonian approximation for gamma-encoded pulse sequences. The validity of the analytical solutions is tested by numerical simulations. The internuclear distance in a (13)C(2)-labelled retinal is estimated by fitting the analytical curves to experimental double-quantum data.     

A comparison of T2*-weighted magnitude and phase imaging for measuring the arterial input function in the rat aorta following intravenous injection of gadolinium contrast agent

The arterial input function (AIF) is important for quantitative MR imaging perfusion experiments employing Gd contrast agents. This study compared the accuracy of T(2)*-weighted magnitude and phase imaging for noninvasive measurement of the AIF in the rat aorta. Twenty-eight in vivo experiments were performed involving simultaneous arterial blood sampling and MR imaging following Gd injection. In vitro experiments were also performed to confirm the in vivo results. At 1.89 T and TE=3 ms, the relationship between changes in 1/T(2)* in blood (estimated from MR signal magnitude) and Gd concentration ([Gd]) was measured to be approximately 19 s(-1) mM(-1), while that between phase and [Gd] was approximately 0.19 rad mM(-1). Both of these values are consistent with previously published results. The in vivo phase data had approximately half as much scatter with respect to [Gd] than the in vivo magnitude data (r(2)=.34 vs. r(2)=.17, respectively). This is likely due to the fact that the estimated change in 1/T(2)* is more sensitive than the phase to a variety of factors such as partial volume effects and T(1) weighting. Therefore, this study indicates that phase imaging may be a preferred method for measuring the AIF in the rat aorta compared to T(2)*-weighted magnitude imaging.     

Photo-physical and structural interactions between viologen phosphorus-based dendrimers and human serum albumin

This work deals with photo-physical and structural interactions between viologen phosphorus dendrimers and human serum albumin (HSA). Viologens are derivatives of 4,4′-bipyridinium salts. Aiming to rationalize the parameters governing such interactions eight types of these polycationic dendrimers in which the generation, the number of charges, the nature of the core and of the terminal groups vary from one to another, were designed and used. The influence of viologen-based dendrimers' on human serum albumin has been investigated. The photo-physical interactions of the two systems have been monitored by fluorescence quenching of free l-tryptophan and of HSA tryptophan residue. Additionally, using circular dichroism (CD) the effect of dendrimers on the secondary structure of albumin was measured. The obtained results show that viologen dendrimers interact with human serum albumin quenching its fluorescence either by collisional (dynamic) way or by forming complexes in a ground state (static quenching). In some cases the quenching is accompanied by changes of the secondary structure of HSA.     

The Electron Density is Smooth Away from the Nuclei

We prove that the electron densities of electronic eigenfunctions of atoms and molecules are smooth away from the nuclei.     

Evolution of the ring-like vortices and spike structure in transitional boundary layers

At the late stage of transitional boundary layers, the nonlinear evolution of the ring-like vortices and spike structures and their effects on the surrounding flow were studied by means of direct numerical simulation with high order accuracy. A spatial transition of the flat-plate boundary layers in the compressible flow was conducted. Detailed numerical results with high resolution clearly represented the typical vortex structures, such as ring-like vortices and so on, and induced ejection and sweep events...     

Hypoxia-induced transcription of dopamine D3 and D4 receptors in human neuroblastoma and astrocytoma cells

Background

The aim of this study is to examine the influence of the catechol-O-methyltranferase (COMT) gene (polymorphism Val158 Met) as a risk factor for Alzheimer's disease (AD) and mild cognitive impairment of amnesic type (MCI), and its synergistic effect with the apolipoprotein E gene (APOE). A total of 223 MCI patients, 345 AD and 253 healthy controls were analyzed. Clinical criteria and neuropsychological tests were used to establish diagnostic groups. The DNA Bank of the University of the Basque Country (UPV-EHU) (Spain) determined COMT Val158 Met and APOE genotypes using real time polymerase chain reaction (rtPCR) and polymerase chain reaction (PCR), and restriction fragment length polymorphism (RFLPs), respectively. Multinomial logistic regression models were used to determine the risk of AD and MCI.

Results

Neither COMT alleles nor genotypes were independent risk factors for AD or MCI. The high activity genotypes (GG and AG) showed a synergistic effect with APOE ε4 allele, increasing the risk of AD (OR = 5.96, 95%CI 2.74-12.94, p < 0.001 and OR = 6.71, 95%CI 3.36-13.41, p < 0.001 respectivily). In AD patients this effect was greater in women. In MCI patients such as synergistic effect was only found between AG and APOE ε4 allele (OR = 3.21 95%CI 1.56-6.63, p = 0.02) and was greater in men (OR = 5.88 95%CI 1.69-20.42, p < 0.01).

Conclusion

COMT (Val158 Met) polymorphism is not an independent risk factor for AD or MCI, but shows a synergistic effect with APOE ε4 allele that proves greater in women with AD.     

Cytogenetics of four Omophoita species (Coleoptera,Chrysomelidae, Alticinae): A comparative analysis using mitotic and meiotic cells submitted to the standard staining and C-banding technique

Omophoita belongs to the tribe Oedionychini and is endemic from Neotropical region. The species of the tribe Oedionychini have revealed certain singular chromosomal features, such as sex chromosomes with extremely large size, asynapsis, and synthelic or amphithelic orientation during meiosis. Additionally, some species also showed post-reductional segregation of the gigantic sex chromosomes in meiotic division. The purpose of this work was to characterize cytogenetically four Omophoita species (O. magniguttis, O. octoguttata, O. personata, and O. sexnotata) in relation to their diploid number, chromosomal morphology, type of sex chromosome system, and constitutive heterochromatin pattern in mitotic and meiotic cells, and compare the obtained data with those of related species to establish the mechanism involved in the chromosomal differentiation of these species during the evolutionary process. The diploid number, 2n = 22 = 20 + X + y, and meiotic formulae, 10II + X + y, observed in these species were similar to those of the same genus and other species related. The autosomal morphology was acrocentric in O. magniguttis and O. octoguttata, metacentric in O. personata, and predominantly metacentric in O. sexnotata. In all these species, the sex chromosomes were metacentric. The secondary constriction occurred in pair 6 and X chromosome of O. personata, and in pair 6 and y chromosome of O. sexnotata. The constitutive heterochromatin was pericentromeric in O. magniguttis and centromeric in O. sexnotata, with the exception of the mitotic sex chromosomes of O. sexnotata, in which centromeric C band was lacking. Additional C bands in the sex chromosomes of O. magniguttis and certain autosomes and sex chromosomes of O. sexnotata were observed. Collochores were indirectly identified in the spermatocytes of O. octoguttata, O. personata, and O. sexnotata. The main mechanisms involved in the karyotype evolution of these species were discussed.     

Impairment of mitochondrial calcium handling in a mtSOD1 cell culture model of motoneuron disease

Background  

Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder characterized by the selective loss of motor neurons (MN) in the brain stem and spinal cord. Intracellular disruptions of cytosolic and mitochondrial calcium have been associated with selective MN degeneration, but the underlying mechanisms are not well understood. The present evidence supports a hypothesis that mitochondria are a target of mutant SOD1-mediated toxicity in familial amyotrophic lateral sclerosis (fALS) and intracellular alterations of cytosolic and mitochondrial calcium might aggravate the course of this neurodegenerative disease. In this study, we used a fluorescence charged cool device (CCD) imaging system to separate and simultaneously monitor cytosolic and mitochondrial calcium concentrations in individual cells in an established cellular model of ALS.