Reversed-phase high-performance liquid chromatography versus spectrophotometric assay for thimerosal in Cuban recombinant hepatitis B vaccine

A reversed-phase liquid chromatographic method was applied to study the stability of thimerosal in Cuban recombinant hepatitis B vaccine samples stored under different temperature conditions. Salicylic acid was used as internal standard, it allowed one to determine the thimerosal in the presence of its degradation products. Good stability of the preservative was demonstrated in vaccine samples for as long as 6 years. The same results were obtained when the vaccine samples were incubated at 37 and 45 degrees C during 30 days. The results were in compliance with the microbiological test for determining the effectiveness of antimicrobial preservative in these samples.     

Mannich-type reactions in the gas-phase: the addition of enol silanes to cyclic N-acyliminium ions

The intrinsic gas-phase reactivity of cyclic N-acyliminium ions in Mannich-type reactions with the parent enol silane, vinyloxytrimethylsilane, has been investigated by double- and triple-stage pentaquadrupole mass spectrometric experiments. Remarkably distinct reactivities are observed for cyclic N-acyliminium ions bearing either endocyclic or exocyclic carbonyl groups. NH-Acyliminium ions with endocyclic carbonyl groups locked in s-trans forms participate in a novel tandem N-acyliminium ion reaction: the nascent adduct formed by simple addition is unstable and rearranges by intramolecular trimethylsilyl cation shift to the ring nitrogen, and an acetaldehyde enol molecule is eliminated. An NSi(CH(3))(3)-acyliminium ion is formed, and this intermediate ion reacts with a second molecule of vinyloxytrimethylsilane by simple addition to form a stable acyclic adduct. N-Acyl and N,N-diacyliminium ions with endocyclic carbonyl groups, for which the s-cis conformation is favored, react distinctively by mono polar [4(+) + 2] cycloaddition yielding stable, ressonance-stabilized cycloadducts. Product ions were isolated via mass-selection and structurally characterized by triple-stage mass spectrometric experiments. B3LYP/6-311G(d,p) calculations corroborate the proposed reaction mechanisms.     

The d = [δ, b] Decomposition of Topological Yang–Mills Theory

We review the construction of topological Yang–Mills theory (TYMT) from the point of view of an operator satisfying d = [, b] with b the BRST operator. We focus our attention on a situation in which [, d] 0 and show how this leads us to consider a more general derivative ~d = b + d + _{k 2 k} ^1–k .     

Field quantization in non-archimedean field theory

A formulation of field quantization of bosonic and fermionic fields defined on the disconnected field of p-acid numbers Q _p is given. We introduce a canonical quantization procedure and exhibit the properties of the nonlocal operators present in the action. Its relevance to string theory on Q _p is also discussed.     

Brans-Dicke cosmology with time-dependent cosmological term

Berman and Som's solution for a Brans-Dicke cosmology with time-dependent cosmological term, Robertson-Walker metric, perfect fluid, and perfect gas law of state solves the horizon, homogeneity, and isotropy problems without requiring any unnatural fine tuning in the very early universe, thus being an alternative model to inflation. The model also does not need recourse to quantum cosmology, and solves the flatness and magnetic monopole problems.     

A wave traveling over a Hopf instability shapes the cochlear tuning curve

The tuning curve of the cochlea measures how intense an input is required to elicit a given output level as a function of the frequency. It is a fundamental object of auditory theory, for it summarizes how to identify sounds on the basis of the cochlear output. A simple model is presented showing that only two elements are sufficient for establishing the cochlear tuning curve: a broadly tuned traveling wave, moving unidirectionally from high to low frequencies, and a set of mechanosensors poised at the threshold of an oscillatory (Hopf) instability. These two components generate the various frequency-response regimes needed for a cochlear tuning curve with a high slope.     

Anisotropic Stars: Exact Solutions

We study the effects of anisotropic pressure on the properties of spherically symmetric, gravitationally bound objects. We consider the full general-relativistic treatment of this problem and obtain exact solutions for various forms of the equation of state connecting the radial and tangential pressures. It is shown that pressure anisotropy can have significant effects on the structure and properties of stellar objects. In particular, the maximum value of 2M / R can approach unity (2M / R < 8/9 for isotropic objects) and the surface redshift can be arbitrarily large.     

Brans-Dicke static universes

We study flat and nonflat static models obeying the Brans-Dicke theory and RW metric, including the case which considers Bertolami's time-dependent cosmological term. We find several solutions where the density remains constant, while the gravitational constant varies with time.     

A simple procedure for deriving an electrical double layer equation from the Born—Green—Yvon theory

A simple procedure for deriving electrical double layer equations from electrolyte theories is applied to the Born—Green—Yvon equation. It is shown that when the auto-field effects are not considered in the wall-ion potential, the equation obtained with this procedure leads to gross inconsistencies.