N-Demethyl-sauroxine,a novel Lycodine Group alkaloid from Huperzia saururus

The present study describes the isolation and identification of N-demethyl-sauroxine, a novel Lycopodium alkaloid obtained from Huperzia saururus (Lam.) Trevis. (Lycopodiaceae). Its structure and relative stereochemistry were elucidated on the basis of its spectral data and chemical correlations. Additionally, acetylcholinesterase inhibitory activity was evaluated (IC₅₀ = 209.6 ± 1.1 μM). The structure of the already identified alkaloid sauroxine was also re-validated through two dimensional NMR data.     

Cortical diversity in three species groups of Parmotrema sensu lato (Parmeliaceae,lichenized Ascomycota)

Conventional techniques for structural studies under light microscope were employed to describe and compare the upper cortex anatomy in three species groups of Parmotrema sensu lato. This study indicates that there is a pattern in the structure of the upper cortex in all the three groups of Parmotrema species studied here, and this pattern can be used to place each species group separately within the genus Parmotrema. Although the species of Parmotrema studied have palisade prosoplectenchymatous upper cortex, there are clear differences between the analyzed groups. The upper cortex of Parmotrema s. str. can be characterized by more elongated cells and very compressed hyphae with small interstices between them; the upper cortex of the species with reticular maculae is characterized by the presence of maculae produced by the organization of hyphae that rise towards to the upper cortex and that are not so compressed, while the upper cortex of the species with dimorphic rhizinae has less elongated almost-rounded cells without interstices or cracks but is generally accompanied by aeroplectenchyma. Besides, the first cell layer of the upper cortex presents differential staining by toluidine blue and the epicortex is tightly attached to it.     

Infrared and X-ray simultaneous spectroscopy: a novel conceptual beamline design for time resolved experiments

Many physical/chemical processes such as metal–insulator transitions or self-assembly phenomena involve correlated changes of electronic and atomic structure in a wide time range from microseconds to minutes. To investigate these dynamic processes we not only need a highly brilliant photon source in order to achieve high spatial and time resolution but new experimental methods have to be implemented. Here we present a new optical layout for performing simultaneous or concurrent infrared and X-ray measurements. This approach may indeed return unique information for example the interplay between structural changes and chemical processes occurring in the investigated sample. A beamline combining two X-ray and IR beams may really take advantage of the unique synchrotron radiation properties: the high brilliance and the broad spectrum. In this contribution we will describe the conceptual layout and the expected performance of a complex system designed to collect IR and X-ray radiation from the same bending magnet on a third-generation synchrotron radiation ring. If realized, this beamline will enable time-resolved spectroscopy experiments offering new scientific opportunities at the frontiers of science.     

Large-scale structure of passive scalar turbulence

We investigate the large-scale statistics of a passive scalar transported by a turbulent velocity field by means of direct numerical simulations. We focus on scales larger than the characteristic length scale of scalar injection, yet smaller than the correlation length of the velocity. We show the existence of nontrivial long-range correlations in the form of new power laws for the decay of high-order coarse-grained scalar cumulants. This result contradicts the classical scenario of Gibbs equilibrium statistics that should hold in the absence of scalar flux. The breakdown of "thermal equilibrium" at large scales is traced back to the statistical geometry of turbulent dispersion of two scalar blobs. The numerical values obtained for the scaling exponents of the coarse-grained scalar cumulants are in agreement with recent theoretical results.     

On Graphlike k-Dissimilarity Vectors

Let \({\mathcal{G} = (G, w)}\) be a positive-weighted simple finite connected graph, that is, let G be a simple finite connected graph endowed with a function w from the set of edges of G to the set of positive real numbers. For any subgraph \({G^\prime}\) of G, we define \({w(G^\prime)}\) to be the sum of the weights of the edges of \({G^\prime}\) . For any i ₁, . . . , i _k vertices of G, let \({D_{\{i_1,..., i_k\}} (\mathcal{G})}\) be the minimum of the weights of the subgraphs of G connecting i ₁, . . . , i _k . The \({D_{\{i_1,..., i_k\}}(\mathcal{G})}\) are called k-weights of \({\mathcal{G}}\) . Given a family of positive real numbers parametrized by the k-subsets of {1, . . . , n}, \({{\{D_I\}_{I} \in { \{1,...,n\} \choose k}}}\) , we can wonder when there exist a weighted graph \({\mathcal{G}}\) (or a weighted tree) and an n-subset {1, . . . , n} of the set of its vertices such that \({D_I (\mathcal{G}) = D_I}\) for any \({I} \in { \{1,...,n\} \choose k}\) . In this paper we study this problem in the case k =  n?1.     

Effect of some physico-chemical conditions on an immunoassay for viable Escherichia coli

Viable Escherichia coli can be detected by an immunoassay in which live bacteria captured on antibody-coated paramagnetic beads are induced to synthesize the enzyme β-galactosidase, which catalyzes the hydrolysis of the slightly fluorescent substrate 4-methyl umbelliferyl-β-d-galactoside to the highly fluorescent product 7-hydroxy-4-methylcoumarin for detection. The effects of bacterial strain, presence of dead bacteria, and some environmental stresses on assay performance were evaluated.     

Chemistry and biology of glycosaminoglycans in blood coagulation

Glycosaminoglycans (GAGs) are widely distributed in animal tissues where they are usually associated with proteins. Six types are commonly recognized: heparin (Hep), heparan sulfate (HS), dermatan sulfate (DS), chondroitin sulfate (Ch-S), keratan sulfate (KS) and hyaluronic acid (Hyal). They are structurally related with a carbohydrate backbone consisting of alternating hexuronic acid (L-iduronic acid and/or D-glucuronic acid) or galactose units and hexosamine (D-glucosamine or D-galactosamine) residues. All GAGs, except Hyal, show sulfate groups along their chains. Certain sulfate glycoaminoglycans have the ability to interfere with blood coagulation, as demonstrated by the extensive clinical use of Hep as an anticoagulant agent. HS and DS show a good anticoagulant activity, although weaker than that of Hep. In contrast, Ch-S has a low ability to inhibit plasma serine proteases, and KS and Hyal are devoid of any effect on coagulation cascade. The interaction between blood coagulation serine proteases and GAGs can be found to have two principle mechanisms: the specific “lock and key” binding and the nonspecific cooperative electrostatic association. This different ability of GAGs to interact with coagulation cascade proteins depends on the molecular weight, the ratio of iduronic/glucoronic acid and the sulfation degree. Many attempts have been made to improve or induce anticoagulant activity of natural GAGs-by chemical modification. Increasing sulfation degree of DS and Ch-S is followed by their biological activity increasing. Hyal, which is devoid of any anticoagulant effect, acquires a good ability to inactivate plasma serine proteases, i.e. thrombin and Factor Xa, when it is sulfated. This ability increases by increasing the number of sulfate groups per disaccharide unit, although the mechanism of action is different from that of Hep, but seems to be independent of its molecular weight.     

Schwarzschild Metrics and Quasi-Universes

The exterior and interior Schwarzschild solutions are rewritten replacing the usual radial variable with an angular one. This allows us to obtain some results that otherwise are less apparent or even hidden in other coordinate systems.