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排序方式: 共有335条查询结果,搜索用时 31 毫秒
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Dr. Mara Beltrán-Gastélum Dr. Berta Esteban-Fernández de Ávila Dr. Hua Gong Dr. Pooyath Lekshmy Venugopalan Prof. Dr. Tibor Hianik Prof. Dr. Joseph Wang Veronika Subjakova 《Chemphyschem》2019,20(23):3177-3180
Herein, we report ultrasound-propelled graphene-oxide coated gold nanowire motors, functionalized with fluorescein-labeled DNA aptamers (FAM-AIB1-apt), for qualitative detection of overexpressed AIB1 oncoproteins in MCF-7 breast cancer cells. The movement of nanomotors under the ultrasound field facilitated intracellular uptake and resulted in a faster aptamer binding with the target protein and thus faster fluorescence recovery. The propulsion behavior of the aptamer functionalized nanomotors greatly enhanced the fluorescence intensity compared to static conditions. The new aptamer@nanomotor-based strategy offers considerable potential for further development of sensing methodologies towards diagnosis of breast cancer. 相似文献
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Altıntas Yemliha Öztürk Esra Aksöz Sezen Keşlioğlu Kâzım Maraşlı Necmettin 《Journal of Thermal Analysis and Calorimetry》2015,122(1):65-72
Journal of Thermal Analysis and Calorimetry - The equilibrated grain boundary groove shapes for solid Bi (Bi–2.87 at.%Ag) in equilibrium with Bi–Ag eutectic liquid have been... 相似文献
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Thermal conductivity variations with temperature of solid phases for lauric acid (LA), myristic acid (MA), pivalic acid (PA), and stearic acid (SA) have been measured with radial heat-flow method. Temperature dependencies of the thermal conductivity for same organic materials have been obtained by linear regression analysis. From graphs of thermal conductivity versus temperature, the thermal conductivity of solid phase at their melting temperature and temperature coefficients of thermal conductivity for LA, MA, PA, and SA have been found to be 0.37, 0.39, 0.23, and 0.35 W K?1 m?1 and 0.00935, 0.00446, 0.01095, and 0.00295 K?1, respectively. The ratios of thermal conductivity of liquid phase to thermal conductivity of solid phase for LA, MA, PA, and SA have also been measured to be 0.52, 0.48, 0.25, and 0.59, respectively, with a Bridgman-type directional solidification apparatus. 相似文献
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Active Intracellular Delivery of a Cas9/sgRNA Complex Using Ultrasound‐Propelled Nanomotors
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Malthe Hansen‐Bruhn Dr. Berta Esteban‐Fernández de Ávila Dr. Mara Beltrán‐Gastélum Prof. Jing Zhao Dr. Doris E. Ramírez‐Herrera Pavimol Angsantikul Prof. Kurt Vesterager Gothelf Prof. Liangfang Zhang Prof. Joseph Wang 《Angewandte Chemie (International ed. in English)》2018,57(10):2657-2661
Direct and rapid intracellular delivery of a functional Cas9/sgRNA complex using ultrasound‐powered nanomotors is reported. The Cas9/sgRNA complex is loaded onto the nanomotor surface through a reversible disulfide linkage. A 5 min ultrasound treatment enables the Cas9/sgRNA‐loaded nanomotors to directly penetrate through the plasma membrane of GFP‐expressing B16F10 cells. The Cas9/sgRNA is released inside the cells to achieve highly effective GFP gene knockout. The acoustic Cas9/sgRNA‐loaded nanomotors display more than 80 % GFP knockout within 2 h of cell incubation compared to 30 % knockout using static nanowires. More impressively, the nanomotors enable highly efficient knockout with just 0.6 nm of the Cas9/sgRNA complex. This nanomotor‐based intracellular delivery method thus offers an attractive route to overcome physiological barriers for intracellular delivery of functional proteins and RNAs, thus indicating considerable promise for highly efficient therapeutic applications. 相似文献
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Inside Cover: Active Intracellular Delivery of a Cas9/sgRNA Complex Using Ultrasound‐Propelled Nanomotors (Angew. Chem. Int. Ed. 10/2018)
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