Combined effect of hydroxypropyl methylcellulose and hydroxypropyl-β-cyclodextrin on physicochemical and dissolution properties of celecoxib

Investigation on the influence of hydroxypropyl methylcellulose (HPMC) on solubility and dissolution properties of celecoxib/hydroxypropyl-β-cyclodextrin system was carried out, with the ultimate goal of enhancing the drug bioavailability. ¹H-NMR and ¹³C-NMR spectroscopy were first performed to elucidate the type of interactions between celecoxib (CEL) and hydroxypropyl-β-cyclodextrin (HP-β-CD). Then, solubility studies in the absence and in the presence of HPMC were carried out in aqueous solution. After heating in autoclave of CEL/HP-β-CD/HPMC suspensions a synergistic increasing effect on the aqueous solubility of CEL was observed. In fact, the presence of both HP-β-CD (0.05 M) and HPMC (0.25% w/v) gave rise to a 330-fold CEL solubility increase, whereas the cyclodextrin alone provided a 34-fold increase. Gibbs free energy values calculated from phase solubility data were all negative, indicating the spontaneous nature of CEL solubilization, and they decreased in the presence of HPMC, demonstrating that the solubilization conditions became more favorable. CEL/HP-β-CD and CEL/HP-β-CD/HPMC solid systems (physical mixtures and coevaporated products) were characterized by differential scanning calorimetry and infrared spectroscopy. Results suggested that the coevaporation method yields a high degree of amorphous entities and indicated the formation of a CEL/HP-β-CD complex in the coevaporated products. The positive effect of HPMC is particularly evident when looking at the CEL dissolution rate from the binary and ternary solid systems. Specifically, the percent of CEL dissolved after 10 min. resulted 84.21% for ternary coevaporated product and 50.18% for binary coevaporated product with respect to 13.10% for the drug alone.     

Biaxial orientation of polyamide films: processability and properties

The production of biaxially oriented films can be performed with two techniques: the tenter frame and the double bubble process. The most important difference between the two processes is that in tubular orientation the MD and TD stretching are simultaneous. This event is very important specially in the case of biaxially oriented polyamide films to avoid hydrogen bonding which occur during the first stretching stage of tenter frame process and cause resistance to subsequent transverse direction orientation (1-4). Double bubble process consists in the stretching of a tubular film where the stretching ratios in the machine and transverse directions are determined by inside bubble pressure and the difference in roll speeds between the two sets of nips rolls that contain the bubble. The work presented here reports some results about double bubble polyamide films realised with a laboratory scale system. The study concerns the processability (5-6) of two different polyamides: a nylon 6 (PA6) and a copolymer of the nylon 6 with isophthalic acid and isophorondiamine (PA6 - IPA.IPD) at 5% w/w of comonomer. Two main process arrangements where adopted differing for first bubble cooling technique: a water cooling and a air cooling. The films produced in both conditions were then characterised with a particular regard to mechanical and barrier performances. Morphological analyses were also carried out in order to correlate the process conditions to structure and properties of films.     

Crystalline orientation and molecular transport properties in nanoporous syndiotactic polystyrene films

The orientation of the crystalline δ nanoporous phase in syndiotactic polystyrene films, obtained by different procedures, have been characterized. For both solution cast and biaxially stretched films a high degree of uniplanar orientation, corresponding to the tendency of the ac crystallographic planes, to be parallel to the film plane has been observed and rationalized. According to molecular dynamics simulations of diffusion of small molecules into the δ nanoporous phase, this uniplanar orientation would minimize the molecular diffusivity through the nanoporous crystalline phase.     

Neuropeptide delivery to the brain: a von Willebrand factor signal peptide to direct neuropeptide secretion

Background  

Multiple neuropeptides, sometimes with opposing functions, can be produced from one precursor gene. To study the roles of the different neuropeptides encoded by one large precursor we developed a method to overexpress minigenes and establish local secretion.     

On the rate of convergence of solutions in domain with periodic multilevel oscillating boundary

In this paper we deal with the homogenization problem for the Poisson equation in a singularly perturbed domain with multilevel periodically oscillating boundary. This domain consists of the body, a large number of thin cylinders joining to the body through the thin transmission zone with rapidly oscillating boundary. Inhomogeneous Fourier boundary conditions with perturbed coefficients are set on the boundaries of the thin cylinders and on the boundary of the transmission zone. We prove the homogenization theorems and derive the estimates for the convergence of the solutions. Copyright © 2010 John Wiley & Sons, Ltd.     

Hierarchical differential evolution for minimal cut sets identification: Application to nuclear safety systems

In this paper, we present a Hierarchical Differential Evolution (HDE) algorithm for minimal cut set (mcs) identification of coherent and non-coherent Fault Trees (FTs). In realistic application of large-size systems, problems may be encountered in handling a large number of gates and events. In this work, to avoid any approximation, mcs identification is originally transformed into a hierarchical optimization problem, stated as the search for the minimum combination of cut sets that can guarantee the best coverage of all the minterms that make the system fail: during the first step of the iterative search, a multiple-population, parallel search policy is used to expedite the convergence of the second step of the exploration algorithm. The proposed hierarchical method is applied to the Reactor Protection System (RPS) of a Pressurized Water Reactor (PWR) and to the the Airlock System (AS) of a CANadian Deuterium Uranium (CANDU) reactor. Results are evaluated with respect to the accuracy and computational demand of the solution found.     

The Glucosamine-derivative NAPA Suppresses MAPK Activation and Restores Collagen Deposition in Human Diploid Fibroblasts Challenged with Environmental Levels of UVB

The ultraviolet (UV) component of solar radiation is the driving force of life on earth, but it can cause photoaging and skin cancer. In this study, we investigated the effects of the glucosamine-derivative 2-(N-Acetyl)-L-phenylalanylamido-2-deoxy-β-D-glucose (NAPA) on human primary fibroblasts (FBs) stimulated in vitro with environmental levels of UVB radiation. FBs were irradiated with 0.04 J cm⁻² UVB dose, which resulted a mild dosage as shown by the cell viability and ROS production measurement. This environmental UVB dose induced activation of MAP kinase ERK 1/2, the stimulation of c-fos and at lower extent of c-jun, and in turn AP-1-dependent up-regulation of pro-inflammatory factors IL-6 and IL-8 and suppression of collagen type I expression. On the contrary, 0.04 J cm⁻² UVB dose was not able to stimulate metalloprotease production. NAPA treatment was able to suppress the up-regulation of IL-6 and IL-8 via the inhibition of MAP kinase ERK phosphorylation and the following AP-1 activation, and was able to attenuate the collagen type I down-regulation induced by the UVBs. Taken together, our results show that NAPA, considering its dual action on suppression of inflammation and stimulation of collagen type I production, represents an interesting candidate as a new photoprotective and photorepairing agents.     

Hypertopologies on function spaces

The aim of this paper is to continue Naimpally’s seminal papers [16], [17], [18], i.e. we investigate topological properties of spaces which force the coincidence of convergences of functions associated with different hyperspace topologies. For example a metric spaceX is locally compact iff the topological convergence and the convergence induced by the Fell topology coincide onC(X,IR). Moreover, the proximal topology on the space of functions, not necessarily continuous, is studied in great detail.     

Polysaccharide nanocrystals as fillers for PLA based nanocomposites

The development of green nanocomposites based on biopolymers and bio-based nanofillers has attracted over the recent years the attention of academic and industrial research. Indeed, these nanocomposites could replace some oil-derived polymers and thus helping to overcome environmental problems. In this regard, PLA as matrix and polysaccharide nanocrystals as fillers are the most promising components to obtain high-performance green bio-nanocomposites suitable for different applications, particularly for packaging and biomedical applications. Indeed, at present, due to its processability, mechanical and biological properties, as well as its commercial availability, poly(lactic acid) (PLA) possesses one of the highest potentials among biopolymers whereas polysaccharide nanocrystals can be considered the most promising bio-based reinforcements due to their availability, renewability, versatility, biodegradability and high aspect ratio. Aim of this review is to give an overview on the preparation routes and main properties of PLA/polysaccharide nanocomposites highlighting the main differences among the three main polysaccharide nanocrystals, i.e. cellulose, chitin, and starch.     

Capturing multi-regime combustion in turbulent flames with a virtual chemistry approach

Multiple flame regimes are encountered in industrial combustion chambers, where premixed, stratified and non-premixed flame regions may coexist. To obtain a predictive tool for pollutant formation predictions, chemical flame modeling must take into account the influence of such complex flame structure. The objective of this article is to apply and compare two reduced chemistry models on both laminar and turbulent multi-regime flame configurations in order to analyze their capabilities in predicting flame structure and CO formation. The challenged approaches are (i) a premixed flamelet-based tabulated chemistry method, whose thermochemical variables are parameterized by a mixture fraction and a progress variable, and (ii) a virtual chemical scheme which has been optimized to retrieve the properties of canonical premixed and non-premixed 1-D laminar flames. The methods are first applied to compute a series of laminar partially-premixed methane-air counterflow flames. Results are compared to detailed chemistry simulations. Both approaches reproduced the thermal flame structure but only the virtual chemistry captures the CO formation in all ranges of equivalence ratio from stoichiometry premixed flame to pure non-premixed flame. Finally, the two chemical models combined with the Thickened Flame model for LES are challenged on a piloted turbulent jet flame with inhomogeneous inlet, the Sydney inhomogeneous burner. Mean and RMS of temperature and CO mass fraction radial profiles are compared to available experimental data. Scatter data in mixture fraction space and Wasserstein metric of numerical and experimental data are also studied. The analyses confirm again that the virtual chemistry approach is able to account for the impact of multi-regime turbulent combustion on the CO formation.