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101.
l-Proline (10 mol %) was found to be a versatile organocatalyst for the selective synthesis of 2-aryl-1-arylmethyl-1H-benzimidazoles from a wide range of substituted o-phenylenediamines and aldehydes in moderate to excellent isolated yields (32-95%) under mild conditions using chloroform as a solvent at ambient temperature. 相似文献
102.
Propranolol, a β‐adrenergic receptor antagonist, is a chiral compound that is marketed as a racemate, but only the (S)‐(?)‐enantiomer is responsible for the β‐adrenoceptor blocking activity. Different chromatographic methods have been applied for separation and determination of enantiomers of (RS)‐propranolol. In this article a review is presented on different liquid chromatographic methods used for enantioseparation of (RS)‐propranolol, using both HPLC and TLC. In addition, some aspects of enantioseparation under achiral phases of liquid chromatography have been briefly mentioned. 相似文献
103.
Binding and condensation of plasmid DNA onto functionalized carbon nanotubes: toward the construction of nanotube-based gene delivery vectors 总被引:10,自引:0,他引:10
Singh R Pantarotto D McCarthy D Chaloin O Hoebeke J Partidos CD Briand JP Prato M Bianco A Kostarelos K 《Journal of the American Chemical Society》2005,127(12):4388-4396
Carbon nanotubes (CNTs) constitute a class of nanomaterials that possess characteristics suitable for a variety of possible applications. Their compatibility with aqueous environments has been made possible by the chemical functionalization of their surface, allowing for exploration of their interactions with biological components including mammalian cells. Functionalized CNTs (f-CNTs) are being intensively explored in advanced biotechnological applications ranging from molecular biosensors to cellular growth substrates. We have been exploring the potential of f-CNTs as delivery vehicles of biologically active molecules in view of possible biomedical applications, including vaccination and gene delivery. Recently we reported the capability of ammonium-functionalized single-walled CNTs to penetrate human and murine cells and facilitate the delivery of plasmid DNA leading to expression of marker genes. To optimize f-CNTs as gene delivery vehicles, it is essential to characterize their interactions with DNA. In the present report, we study the interactions of three types of f-CNTs, ammonium-functionalized single-walled and multiwalled carbon nanotubes (SWNT-NH3+; MWNT-NH3+), and lysine-functionalized single-walled carbon nanotubes (SWNT-Lys-NH3+), with plasmid DNA. Nanotube-DNA complexes were analyzed by scanning electron microscopy, surface plasmon resonance, PicoGreen dye exclusion, and agarose gel shift assay. The results indicate that all three types of cationic carbon nanotubes are able to condense DNA to varying degrees, indicating that both nanotube surface area and charge density are critical parameters that determine the interaction and electrostatic complex formation between f-CNTs with DNA. All three different f-CNT types in this study exhibited upregulation of marker gene expression over naked DNA using a mammalian (human) cell line. Differences in the levels of gene expression were correlated with the structural and biophysical data obtained for the f-CNT:DNA complexes to suggest that large surface area leading to very efficient DNA condensation is not necessary for effective gene transfer. However, it will require further investigation to determine whether the degree of binding and tight association between DNA and nanotubes is a desirable trait to increase gene expression efficiency in vitro or in vivo. This study constitutes the first thorough investigation into the physicochemical interactions between cationic functionalized carbon nanotubes and DNA toward construction of carbon nanotube-based gene transfer vector systems. 相似文献
104.
Speciation of arsenic in ground water samples: A comparative study of CE-UV, HG-AAS and LC-ICP-MS 总被引:1,自引:0,他引:1
The performance of capillary electrophoresis-ultraviolet detector (CE-UV), hydride generation-atomic absorption spectrometry (HG-AAS) and liquid chromatography-inductively coupled plasma mass spectrometry (LC-ICP-MS) have been compared for the speciation of arsenic (As) in groundwater samples. Two inorganic As species, arsenite (AsIII), arsenate (AsV) and one organo species dimethyl arsenic acid (DMA) were mainly considered for this study as these are known to be predominant in water. Under optimal analytical conditions, limits of detection (LD) ranging from 0.10 (AsIII, AsT) to 0.19 (DMA) μg/l for HG-AAS, 100 (AsIII, DMA) to 500 (AsV) μg/l for CE-UV and 0.1 (DMA, MMA) to 0.2 (AsIII, AsV) μg/l for LC-ICP-MS, allowed the determination of the above three species present in these samples. Results obtained by all the three methods are well correlated (r2 = 0.996*** for total As) with the precision of <5% R.S.D. except CE-UV. The effect of interfering ions (e.g. Fe2+, Fe3+, SO42− and Cl−) commonly found in ground water on separation and estimation of As species were studied and corrected for. Spike recovery was tested and found to be 80-110% at 0.5 μg/l As standard except CE-UV where only 50% of the analyte was recovered. Comparison of these results shows that LC-ICP-MS is the best choice for routine analysis of As species in ground water samples. 相似文献
105.
Maverick AW Laxman RK Hawkins MA Martone DP Fronczek FR 《Dalton transactions (Cambridge, England : 2003)》2005,(1):200-206
The tetradentate Schiff-base ligand SIXH2 (alpha,alpha-bis(salicylimino)-m-xylene), prepared from salicylaldehyde and m-xylylenediamine, forms cofacial binuclear complexes with Pd and Cu. Of the two isomers possible (trans-syn and trans-anti) for M2(SIX)2, these complexes crystallize exclusively as the trans-anti isomer. In ansolvous Pd2(SIX)2, the metal-containing planes are approximately parallel, with PdPd 4.416(1) A. Pd2(SIX)2 also forms a crystalline solvate, in which the molecules adopt a more open conformation with longer metal-metal distances (5.109(1) and 5.112(1) A). The M...M distance is significantly longer in Cu2(SIX)2 (6.653(1) A), because of conformational changes in the m-xylylene moieties and substantial tetrahedral distortion about Cu. 相似文献
106.
K. M. Mathew S. Ravi D. Padmanabhan 《Journal of Radioanalytical and Nuclear Chemistry》2005,265(3):505-506
Summary 4,6-dimethoxy-2-methylsulphonylpyrimidine is a key intermediate for the synthesis of pyrithiobac-sodium, a selective herbicide for cotton plant. 14C labeled pyrithiobac-sodium is required for studying the translocation and metabolism in cotton plants. It was prepared by oxidation of 4,6-dimethoxy-[2-14C]-2-methylmercaptopyrimidine with H5IO6/CrO3 in ethyl acetate at room temperature to give 4,6-dimethoxy-[2-14C]-2-methylsulphonylpyrimidine in high yields. 相似文献
107.
Romeiro NC Albuquerque MG de Alencastro RB Ravi M Hopfinger AJ 《Journal of computer-aided molecular design》2005,19(6):385-400
The p38-mitogen-activated protein kinase (p38-MAPK) plays a key role in lipopolysaccharide-induced tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 (IL-1) release during the inflammatory process, emerging as an attractive target for new anti-inflammatory agents. Four-dimensional quantitative structure-activity relationship (4D-QSAR) analysis [Hopfinger et al., J. Am. Chem. Soc., 119 (1997) 10509] was applied to a series of 33 (a training set of 28 and a test set of 5) pyridinyl-imidazole and pyrimidinyl-imidazole inhibitors of p38-MAPK, with IC50 ranging from 0.11 to 2100 nM [Liverton et al., J. Med. Chem., 42 (1999) 2180]. Five thousand conformations of each analogue were sampled from a molecular dynamics simulation (MDS) during 50 ps at a constant temperature of 303 K. Each conformation was placed in a 2 angstroms grid cell lattice for each of three trial alignments. 4D-QSAR models were constructed by genetic algorithm (GA) optimization and partial least squares (PLS) fitting, and evaluated by leave-one-out cross-validation technique. In the best models, with three to six terms, the adjusted cross-validated squared correlation coefficients, Q2adj, ranged from 0.67 to 0.85. Model D (Q2adj = 0.84) was identified as the most robust model from alignment 1, and it is representative of the other best models. This model encompasses new molecular regions as containing pharmacophore sites, such as the amino-benzyl moiety of pyrimidine analogs and the N1-substituent in the imidazole ring. These regions of the ligands should be further explored to identify better anti-inflammatory inhibitors of p38-MAPK. 相似文献
108.
Kenneth L. Stevenson Pamela B. Bell Ravi S. Dhawale Ott Horvth Attila Horvth 《Radiation Physics and Chemistry》1999,55(5-6):489-496
The ejection of hydrated electrons from 266-nm laser-photoexcited solutions containing Cu(NH3)+3, CuCl2−3, or CuBr2−3 occurs through two pathways on the nanosecond time scale: a prompt ejection (ττ>laser pulsewidth) which follows a first-order rate law. This behavior is consistent with electron ejection from two excited states: the primary CTTS state, and longer-lived triplet species consisting of an exciplex and its precursor. The quantum yields for both prompt and delayed ejection are quite high, in the 0.15–0.4 range. 相似文献
109.
Characterization and application of an electrode modified by mechanically immobilized copper hexacyanoferrate 总被引:1,自引:0,他引:1
A newly modified electrode was prepared by mechanical immobilization of copper hexacyanoferrate (CuHCF) on a graphite electrode.
The modified electrode was characterized by cyclic voltammetric experiments. The effect of different background electrolytes,
pHs and scan rates on the electrochemical behaviour of the electrode has been evaluated. In NH4Cl two reversible redox peaks were observed. The first redox peak corresponding to Cu+/Cu2+ is observed only in this medium. The second redox peak corresponds to the Fe(CN)6
4–/Fe(CN)6
3– couple. Both anodic peaks were used for catalytic oxidation of ascorbic acid. As the anodic current for catalytic oxidation
was proportional to the amount of ascorbic acid, an analytical method was developed for the determination of ascorbic acid
in commercial samples.
Received: 26 May 1998 / Revised: 15 March 1999 / Accepted: 20 March 1999 相似文献
110.
Ranjith Kumar Bakku Ravi Gupta Cheol-Woo Min Sun-Tae Kim Genboku Takahashi Junko Shibato Seiji Shioda Fumiko Takenoya Ganesh Kumar Agrawal Randeep Rakwal 《Molecules (Basel, Switzerland)》2022,27(3)
The present research investigates the tuber proteome of the ‘medicinal’ plant Jerusalem artichoke (abbreviated as JA) (Helianthus tuberosus L.) using a high-throughput proteomics technique. Although JA has been historically known to the Native Americans, it was introduced to Europe in the late 19th century and later spread to Japan (referred to as ‘kiku-imo’) as a folk remedy for diabetes. Genboku Takahashi research group has been working on the cultivation and utilization of kiku-imo tuber as a traditional/alternative medicine in daily life and researched on the lowering of blood sugar level, HbA1c, etc., in human subjects (unpublished data). Understanding the protein components of the tuber may shed light on its healing properties, especially related to diabetes. Using three commercially processed JA tuber products (dried powder and dried chips) we performed total protein extraction on the powdered samples using a label-free quantitate proteomic approach (mass spectrometry) and catalogued for the first time a comprehensive protein list for the JA tuber. A total of 2967 protein groups were identified, statistically analyzed, and further categorized into different protein classes using bioinformatics techniques. We discussed the association of these proteins to health and disease regulatory metabolism. Data are available via ProteomeXchange with identifier PXD030744. 相似文献