全文获取类型
收费全文 | 1971篇 |
免费 | 267篇 |
国内免费 | 194篇 |
专业分类
化学 | 1428篇 |
晶体学 | 22篇 |
力学 | 119篇 |
综合类 | 17篇 |
数学 | 211篇 |
物理学 | 635篇 |
出版年
2024年 | 6篇 |
2023年 | 50篇 |
2022年 | 58篇 |
2021年 | 69篇 |
2020年 | 115篇 |
2019年 | 92篇 |
2018年 | 68篇 |
2017年 | 65篇 |
2016年 | 114篇 |
2015年 | 92篇 |
2014年 | 104篇 |
2013年 | 154篇 |
2012年 | 165篇 |
2011年 | 194篇 |
2010年 | 103篇 |
2009年 | 96篇 |
2008年 | 129篇 |
2007年 | 111篇 |
2006年 | 70篇 |
2005年 | 64篇 |
2004年 | 53篇 |
2003年 | 56篇 |
2002年 | 63篇 |
2001年 | 63篇 |
2000年 | 45篇 |
1999年 | 46篇 |
1998年 | 24篇 |
1997年 | 27篇 |
1996年 | 20篇 |
1995年 | 16篇 |
1994年 | 18篇 |
1993年 | 11篇 |
1992年 | 13篇 |
1991年 | 6篇 |
1990年 | 11篇 |
1989年 | 2篇 |
1988年 | 7篇 |
1987年 | 4篇 |
1986年 | 3篇 |
1985年 | 4篇 |
1984年 | 4篇 |
1983年 | 3篇 |
1982年 | 4篇 |
1981年 | 2篇 |
1979年 | 1篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1975年 | 1篇 |
1973年 | 1篇 |
1957年 | 2篇 |
排序方式: 共有2432条查询结果,搜索用时 0 毫秒
11.
12.
Biosynthesis of polyhydroxyalkanoates (PHAs) consisting of 3-hydroxyalkanoates (3HAs) of 4 to 10 carbon atoms was examined
in metabolically engineered Escherichia coli strains. When the fadA and/or fadB mutant E. coli strains harboring the plasmid containing the Pseudomonas sp. 61-3 phaC2 gene and the Ralstonia eutropha phaAB genes were cultured in Luria-Bertani (LB) medium supplemented with 2 g/L of sodium decanoate, all the recombinant E. coli strains synthesized PHAs consisting of C4, C6, C8, and C10 monomer units. The monomer composition of PHA was dependent on
the E. coli strain used. When the fadA mutant E. coli was employed, PHA containing up to 63 mol% of 3-hydroyhexanoate was produced. In fadB and fadAB mutant E. coli strains, 3-hydroxybutyrate (3HB) was efficiently incorporated into PHA up to 86 mol%. Cultivation of recombinant fadA and/or fadB mutant E. coli strains in LB medium containing 10 g/L of sodium gluconate and 2 g/L of sodium decanoate resulted in the production of PHA
copolymer containing a very high fraction of 3HB up to 95 mol%. Since the material properties of PHA copolymer consisting
of a large fraction of 3HB and a small fraction of medium-chain-length 3HA are similar to those of low-density polyethylene,
recombinant E. coli strains constructed in this study should be useful for the production of PHAs suitable for various commercial applications. 相似文献
13.
相转移催化合成对甲氧基肉桂酸-2-乙基己酯的研究 总被引:5,自引:1,他引:5
对甲氧基肉桂酸 2 乙基己酯是一种有效的UVB防晒剂[1 ,2 ] ,一般采用Claisen Schmidt缩合反应制得[3] ,但由于催化剂KOH处于水相 ,对反应物不能充分起到催化作用导致产率较低[4] 。我们利用乙二醇 (PEG)为相转移催化剂合成立体专一的反式对甲氧基肉桂酸 2 乙基己酯 ,产率提高到 72 .5 %。合成路线如下 :1 实验部分1 .1 仪器与试剂BIO RAPFT45 0红外光谱仪 ,用液膜法测试 ;Brurer AM 30 0超导核磁共振仪 (溶剂DC Cl3) ;MAT/44S ,GC MS型色质联用谱仪 ;试剂均为分析纯 ,上海化学试剂… 相似文献
14.
Feng Liu Si Guo Yuan Xiao Li Wang A.E Polikarpov A.A. Shunkevich 《中国化学快报》2007,18(5):588-590
A fibrous sorbent possessing abundant micropore structure was firstly prepared via post-crosslinking reaction on the PP-ST-DVB original fiber. Its micromorphology and sorptive properties were investigated, and the results demonstrated that the novel fibrous hypererosslinked sorbent has narrow pore-size distribution, small average porous radius (1.90 nm), high specific surface area (362.31 m^2/g), and fine sorptive properties for small organic molecules. 相似文献
15.
Complete 1H and 13C NMR assignments of four new steroidal glycosides from a gorgonian coral Junceella juncea 总被引:2,自引:0,他引:2
Four new cholest-type steroidal glycosides, junceellosides A-D, isolated from the EtOH/CH(2)Cl(2) extracts of the South China Sea gorgonian coral Junceella juncea, were identified. Complete assignments of the (1)H and (13)C NMR chemical shifts for these compounds were achieved by means of one- and two-dimensional NMR techniques, including (1)H-(1)H COSY, HSQC, HMBC and NOESY spectra. 相似文献
16.
17.
Electropolymerized m-phenylenediamine was used as an active coating for immobilizing urease and lectin on a gold-plated thickness-shear-mode (TSM) crystal. To enhance effectiveness of immobilization. a bilayer polymer film composed of polyaniline and poly-m-phenylenediamine was proposed. Compared with single poly-m-phenylenediamine film, the bilayer polymer film gave better results in terms of immobilizing capacity, stability and reproductivity. On this bilayer-film-coated TSM quartz crystal, the amount of immobilized lectin was estimated about 1.8 mug/cm(2). Detection of purified human erythrocytes is demonstrated as an example of potential application of this lectin-modified TSM biosensor in clinic. 相似文献
18.
19.
Coleman KS Bailey SR Fogden S Green ML 《Journal of the American Chemical Society》2003,125(29):8722-8723
Single-walled carbon nanotubes have been cyclopropanated under Bingel reaction conditions, and the functionalized nanotubes have been characterized by atomic force microscopy using "chemical tagging" techniques. 相似文献
20.
A20 murine lymphoma cells undergoing Fas-mediated apoptosis showed increase in the activity of phospholipase D (PLD), which is involved in proliferative or mitogenic cellular responses. Using A20 cell lines that were resistant to Fas-induced apoptosis, we investigated the differential effects of Fas cross-linking on PLD activity and sphingolipid metabolism. The basal PLD activities in all of the selected three Fas-resistant clones (#5, #8, and #11) were about 2~4 folds higher than that of wild type A20 cells. Among the PLD isoforms, PLD2 expression was increased in all of the selected Fas-resistant clones. The Fas downstream signaling events triggered by Fas cross-linking, including the activations of PLD, phosphatidylcholine-specific phospholipase C (PC-PLC), sphingomyelinase (SMase), the increase in diacylglycerol (DAG) and protein phosphorylation levels, and the translocation of protein kinase C to membrane were not changed in both of Fas-resistant clone #5 and #8. In contrast, Fas cross-linking stimulated the activity of PLD, PC-PLC, and SMase, translocation of PKC, and protein phosphorylation in Fas-resistant clone #11, similar to that of wild type cells. We also found that clone #11 had a different Fas sequence encoding Fas B which has been known to inhibit Fas-induced apoptosis. These findings suggest that increased PLD2 expression resulting in increased basal PLD activity and the blockade of Fas downstream signaling cascades may be involved to limit apoptosis induced by Fas cross-linking. 相似文献