首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   61篇
  免费   4篇
化学   35篇
数学   16篇
物理学   14篇
  2022年   2篇
  2019年   1篇
  2018年   2篇
  2017年   2篇
  2016年   2篇
  2015年   1篇
  2014年   8篇
  2013年   3篇
  2012年   6篇
  2011年   1篇
  2010年   5篇
  2009年   2篇
  2008年   2篇
  2007年   1篇
  2006年   2篇
  2005年   6篇
  2004年   1篇
  2003年   1篇
  2002年   2篇
  2000年   3篇
  1999年   1篇
  1997年   1篇
  1996年   2篇
  1994年   2篇
  1993年   1篇
  1992年   2篇
  1989年   1篇
  1986年   1篇
  1980年   1篇
排序方式: 共有65条查询结果,搜索用时 171 毫秒
1.
2.
5‐Vinyl‐2′‐deoxyuridine (VdU) is the first reported metabolic probe for cellular DNA synthesis that can be visualized by using an inverse electron demand Diels–Alder reaction with a fluorescent tetrazine. VdU is incorporated by endogenous enzymes into the genomes of replicating cells, where it exhibits reduced genotoxicity compared to 5‐ethynyl‐2′‐deoxyuridine (EdU). The VdU–tetrazine ligation reaction is rapid (k≈0.02 M ?1 s?1) and chemically orthogonal to the alkyne–azide “click” reaction of EdU‐modified DNA. Alkene–tetrazine ligation reactions provide the first alternative to azide–alkyne click reactions for the bioorthogonal chemical labeling of nucleic acids in cells and facilitate time‐resolved, multicolor labeling of DNA synthesis.  相似文献   
3.
4.
5.

Background  

The archaeal exosome is formed by a hexameric RNase PH ring and three RNA binding subunits and has been shown to bind and degrade RNA in vitro. Despite extensive studies on the eukaryotic exosome and on the proteins interacting with this complex, little information is yet available on the identification and function of archaeal exosome regulatory factors.  相似文献   
6.
We present two linearization-based algorithms for mixed-integer nonlinear programs (MINLPs) having a convex continuous relaxation. The key feature of these algorithms is that, in contrast to most existing linearization-based algorithms for convex MINLPs, they do not require the continuous relaxation to be defined by convex nonlinear functions. For example, these algorithms can solve to global optimality MINLPs with constraints defined by quasiconvex functions. The first algorithm is a slightly modified version of the LP/NLP-based branch-and-bouund \((\text{ LP/NLP-BB })\) algorithm of Quesada and Grossmann, and is closely related to an algorithm recently proposed by Bonami et al. (Math Program 119:331–352, 2009). The second algorithm is a hybrid between this algorithm and nonlinear programming based branch-and-bound. Computational experiments indicate that the modified LP/NLP-BB method has comparable performance to LP/NLP-BB on instances defined by convex functions. Thus, this algorithm has the potential to solve a wider class of MINLP instances without sacrificing performance.  相似文献   
7.
8.
In this study we have used two fluorescent probes, tetrakis(diisopropylguanidino)-zinc-phthalocyanine (Zn-DIGP) and N-methylmesoporphyrin IX (NMM), to monitor the reassembly of “split” G-quadruplex probes on hybridization with an arbitrary “target” DNA. According to this approach, each split probe is designed to contain half of a G-quadruplex-forming sequence fused to a variable sequence that is complementary to the target DNA. Upon mixing the individual components, both base-pairing interactions and G-quadruplex fragment reassembly result in a duplex–quadruplex three-way junction that can bind to fluorescent dyes in a G-quadruplex-specific way. The overall fluorescence intensities of the resulting complexes were dependent on the formation of proper base-pairing interactions in the duplex regions, and on the exact identity of the fluorescent probe. Compared with samples lacking any “target” DNA, the fluorescence intensities of Zn-DIGP-containing samples were lower, and the fluorescence intensities of NMM-containing samples were higher on addition of the target DNA. The resulting biosensors based on Zn-DIGP are therefore termed “turn-off” whereas the biosensors containing NMM are defined as “turn-on”. Both of these biosensors can detect target DNAs with a limit of detection in the nanomolar range, and can discriminate mismatched from perfectly matched target DNAs. In contrast with previous biosensors based on the peroxidase activity of heme-bound split G-quadruplex probes, the use of fluorescent dyes eliminates the need for unstable sensing components (H2O2, hemin, and ABTS). Our approach is direct, easy to conduct, and fully compatible with the detection of specific DNA sequences in biological fluids. Having two different types of probe was highly valuable in the context of applied studies, because Zn-DIGP was found to be compatible with samples containing both serum and urine whereas NMM was compatible with urine, but not with serum-containing samples.  相似文献   
9.
We hypothesize that the energy strategy of a cell is a key factor for determining how, or if, the immune system interacts with that cell. Cells have a limited number of metabolic states, in part, depending on the type of fuels the cell consumes. Cellular fuels include glucose (carbohydrates), lipids (fats), and proteins. We propose that the cell's ability to switch to, and efficiently use, fat for fuel confers immune privilege. Additionally, because uncoupling proteins are involved in the fat burning process and reportedly in protection from free radicals, we hypothesize that uncoupling proteins play an important role in immune privilege. Thus, changes in metabolism (caused by oxidative stresses, fuel availability, age, hormones, radiation, or drugs) will dictate and initiate changes in immune recognition and in the nature of the immune response. This has profound implications for controlling the symptoms of autoimmune diseases, for preventing graft rejection, and for targeting tumor cells for destruction.  相似文献   
10.
Dynamical interactions between a scanning tip and a silicon substrate are investigated using molecular dynamics simulations of both the constant-height and constant-force scan modes. Localized temporary and permanent modifications of the substrate occur, depending on tip-substrate-strate separation and scan geometry. Implications for resolving structural and force characteristics in scanning tip spectroscopies, employing atomically sharp as well as large ordered of disordered tips are discussed.  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号