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71.
Ana Maria Titoiu Roxana Porumb Pablo Fanjul‐Bolado Petru Epure Medana Zamfir Alina Vasilescu 《Electroanalysis》2019,31(11):2262-2273
In this work we investigate the performance of a simple, disposable electrochemical aptasensor for lysozyme and its usefulness for monitoring the allergen risk along wine production. The sensor relies on screen‐printed gold electrodes modified with gold nanoparticles as the electrochemical transducer, with detection by cyclic voltammetry. This simple method is characterized by a detection limit of 0.32 μg.mL?1 lysozyme and a linear range of 1–10 μg.mL?1, being appropriate for the analysis of lysozyme‐treated wines. Several white wines where sulphur dioxide was partially replaced by lysozyme were produced and analyzed with the aptasensor at critical stages during wine production. The results obtained with the aptasensor were moreover compared with those recorded in parallel by a standard method, high performance liquid chromatography (HPLC). The specific advantages brought by the use of nanomaterial and the limitations of the sensor are discussed. The sensor allowed evaluating the effect of various technological steps along wine production on the content of lysozyme. 相似文献
72.
D.C. Radford C. Baktash A. Galindo-Uribarri C.J. Gross T.A. Lewis P.E. Mueller P.A. Hausladen D. Shapira D.W. Stracener C.-H. Yu B. Fuentes E. Padilla D.J. Hartley C.J. Barton M. Caprio N.V. Zamfir 《The European Physical Journal A - Hadrons and Nuclei》2002,15(1-2):171-173
The Holifield Radioactive Ion Beam Facility at Oak Ridge National Laboratory has recently produced the world's first post-accelerated
beams of heavy neutron-rich nuclei. The first experiments with these beam are described, and the results discussed. B(E2;0+ → 2+) values for neutron-rich 126,128Sn and 132,134,136Te isotopes have been measured by Coulomb excitation in inverse kinematics. The results for 132Te and 134Te (N = 80, 82) show excellent agreement with systematics of lighter Te isotopes, but the B(E2) value for 136Te (N = 84) is unexpectedly small. Single-neutron transfer reactions with a 134Te beam on natBe and 13C targets at energies just above the Coulomb barrier have also been studied.
Received: 21 March 2002 / Accepted: 16 May 2002 / Published online: 31 October 2002
RID="a"
ID="a"e-mail: radford@mail.phy.ornl.gov 相似文献
73.
The parameter-independent (up to overall scale factors) predictions of the X(5)−β
2,X(5)−β
4, and X(3) models, which are variants of the X(5) critical point symmetry developed within the framework of the geometric collective model, are compared to two-parameter
calculations in the framework of the interacting boson approximation (IBA) model. The results show that these geometricmodels
coincide with IBA parameters consistentwith the phase/shape transition region of the IBA for boson numbers of physical interest
(close to 10). 186Pt and 172Os are identified as good examples of X(3), while 146Ce, 174Os, and 158Er, 176Os are identified as good examples of X(5)−β
2 and X(5)−β
4 behavior, respectively
The text was submitted by the authors in English. 相似文献
74.
We report on the development of a novel methodology to extend the limits of capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) general applicability. A sheathless on-line CE-ESI-MS setup was optimized on standard monosaccharide mixture to operate in reverse polarity and negative ion mode for MS detection without pressure to assist the sample migration, coating of the capillaries, and/or sample derivatization. This approach was further applied for screening of a complex glycopeptide mixture obtained from the urine of a patient diagnosed with N-acetylhexosaminidase deficiency, known as Schindler's disease. The potential of this methodology in terms of high sensitivity, separation efficiency, resolution, and reproducibility is demonstrated. In combination with the high quality of MS data, a new, significantly improved insight into the sample heterogeneity is possible. 相似文献
75.
A novel approach in glycosaminoglycomics, based on sheathless on-line capillary electrophoresis/nanoelectrospray ionization-quadrupole time of flight-mass spectrometry (CE/nanoESI-QTOF-MS) and tandem MS of extended chondroitin sulfate/dermatan (CS/DS) oligosaccharide chains is described. The methodology required the construction of a new sheathless CE/nanoESI-QTOF-MS configuration, its implementation and optimization for the high sensitivity analysis of CS/DS oligosaccharide mixtures from conditioned culture medium of decorin transfected human embryonic kidney (HEK) 293 cells. Under newly established sheathless on-line CE/(-)nanoESI conditions for glycosaminoglycan (GAG) ionization and MS detection, single CS/DS oligosaccharide components of extended chain length and increased sulfation degree were identified. Molecular ions corresponding to species carrying 5 and 6 negative charges could be generated for large GAG oligosaccharide species in the negative ion nanoESI-MS. The optimized on-line conditions enabled the detection of molecular ions assigned to oversulfated tetradeca-, octadeca-, and eicosasaccharide CS/DS molecules, which represent the category of largest sulfated GAG-derived oligosaccharides evidenced by CE/ESI-MS. By on-line CE/ESI tandem MS in data-dependent acquisition mode the oversulfated eicosasaccharide species could be sequenced and the localization of the additional sulfate group along the chain could be determined. 相似文献
76.
A recently introduced way of parameterizing the energies of 3 states in many even-even nuclei, based on the microscopic structure of the low-energy octupole mode, is slightly modified so it now includes all (non-doubly magic) nuclei with A ? 30. 相似文献
77.
78.
The use of off-line high-performance capillary electrophoresis in connection with nanospray electrospray ionization quadrupole time-of-flight tandem mass spectrometry for identification of complex carbohydrates of biological origin is presented. The method was applied to the identification of O-glycosylated amino acids and -glycopeptides from the urine of patients suffering from a hereditary disease - N-acetylhexosaminidase deficiency. Structural elements typical for O-glycosylation of proteins, like expression of core 1 and 2 type O-glycans with different numbers of N-acetyllactosaminyl repeats and different degrees of sialylation, can be directly detected. 相似文献
79.
80.
N. V. Zamfir 《Physics of Atomic Nuclei》2001,64(6):1060-1063
The octupole bands in the Z~88, N~134 region, with their abundance of experimental information, offer a real challenge for different nuclear structure models. In this region, quasimolecular bands, which are thought to indicate the presence of stable octupole deformation, have been observed. The quantities most often reported are the B(E1)/B(E2) branching ratios from these levels. The calculations in the framework of the spdf model show that the 1-pf boson limit (dipole-octupole vibrations) can describe the essential experimental features related to the octupole bands in the Rn-Th region. 相似文献