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排序方式: 共有595条查询结果,搜索用时 15 毫秒
591.
Dr. Lorena Mendive-Tapia Dr. Laia Miret-Casals Dr. Nicole D. Barth Jinling Wang Dr. Anne de Bray Dr. Massimiliano Beltramo Vincent Robert Prof. Dr. Christophe Ampe Prof. Dr. David J. Hodson Prof. Dr. Annemieke Madder Prof. Dr. Marc Vendrell 《Angewandte Chemie (International ed. in English)》2023,62(20):e202302688
The G protein-coupled kisspeptin receptor (GPR54 or KISS1R) is an important mediator in reproduction, metabolism and cancer biology; however, there are limited fluorescent probes or antibodies for direct imaging of these receptors in cells and intact tissues, which can help to interrogate their multiple biological roles. Herein, we describe the rational design and characterization of a new acid-resistant BODIPY-based amino acid (Trp-BODIPY PLUS), and its implementation for solid-phase synthesis of fluorescent bioactive peptides. Trp-BODIPY PLUS retains the binding capabilities of both short linear and cyclic peptides and displays notable turn-on fluorescence emission upon target binding for wash-free imaging. Finally, we employed Trp-BODIPY PLUS to prepare some of the first fluorogenic kisspeptin-based probes and visualized the expression and localization of GPR54 receptors in human cells and in whole mouse pancreatic islets by fluorescence imaging. 相似文献
592.
Zhiming Cheng Dr. Emily J Thompson Dr. Lorena Mendive-Tapia Dr. Jamie I Scott Dr. Sam Benson Dr. Takanori Kitamura Ana Senan-Salinas Dr. Youhani Samarakoon Dr. Edward W Roberts Dr. Maykel A Arias Prof. Dr. Julian Pardo Dr. Eva M Galvez Prof. Dr. Marc Vendrell 《Angewandte Chemie (International ed. in English)》2023,62(8):e202216142
Cytotoxic immune cells, including T lymphocytes (CTLs) and natural killer (NK) cells, are essential components of the host response against tumors. CTLs and NK cells secrete granzyme A (GzmA) upon recognition of cancer cells; however, there are very few tools that can detect physiological levels of active GzmA with high spatiotemporal resolution. Herein, we report the rational design of the near-infrared fluorogenic substrates for human GzmA and mouse GzmA. These activity-based probes display very high catalytic efficiency and selectivity over other granzymes, as shown in tissue lysates from wild-type and GzmA knock-out mice. Furthermore, we demonstrate that the probes can image how adaptive immune cells respond to antigen-driven recognition of cancer cells in real time. 相似文献
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Safa Almadhi Joe Forth Laura Rodriguez-Arco Aroa Duro-Castano Ian Williams Lorena Ruiz-Pérez Giuseppe Battaglia 《Macromolecular bioscience》2023,23(8):2300068
A bottom-up approach to fabricating monodisperse, two-component polymersomes that possess phase-separated (“patchy”) chemical topology is presented. This approach is compared with already-existing top-down preparation methods for patchy polymer vesicles, such as film rehydration. These findings demonstrate a bottom-up, solvent-switch self-assembly approach that produces a high yield of nanoparticles of the target size, morphology, and surface topology for drug delivery applications, in this case patchy polymersomes of a diameter of ≈50 nm. In addition, an image processing algorithm to automatically calculate polymersome size distributions from transmission electron microscope images based on a series of pre-processing steps, image segmentation, and round object identification is presented. 相似文献