64CuCl2 is an economic radiotracer for oncologic PET investigations. In the present study, we characterized the uptake of 64CuCl2 in vivo by µPET/CT in an allograft 4T1-related mouse model (BALB/c) of advanced breast cancer. 18F-FDG was used as a comparator. Twenty-two animals were imaged 7–9 days following 4T1-cell implantation inside mammary glands. Dynamic 64CuCl2 µPET/CT acquisition or iterative static images up to 8 h p.i. were performed. Animal biodistribution and tumor uptake were first evaluated in vivo by µPET analysis and then assessed on tissue specimens. Concerning 18F-FDG µPET, a static acquisition was performed at 15 min and 60 min p.i. Tumor 64CuCl2 accumulation increased from 5 min to 4 h p.i., reaching a maximum value of 5.0 ± 0.20 %ID/g. Liver, brain, and muscle 64CuCl2 accumulation was stable over time. The tumor-to-muscle ratio remained stable from 1 to 8 h p.i., ranging from 3.0 to 3.7. Ex vivo data were consistent with in vivo estimations. The 18F-FDG tumor accumulation was 8.82 ± 1.03 %ID/g, and the tumor-to-muscle ratio was 4.54 ± 1.11. 64CuCl2 PET/CT provides good characterization of the 4T1-related breast cancer model and allows for exploration of non-glycolytic cellular pathways potentially of interest for theragnostic strategies. 相似文献
Condensations between 3-X-2,4-dimethylpyrroles (X = H, CH3, C2H5, and CO2C2H5) and acyl chlorides gave derivatives of 3,5,3′,5′-tetramethylpyrromethene (isolated as their hydrochloride salts): 6-methyl, 6-ethyl, 4,4′,6-trimethyl, 4,4′-diethyl-6-methyl, and 4,4′-dicarboethoxy-6-ethyl derivatives for conversion on treatment with boron trifluoride to 1,3,5,7-tetramethylpyrromethene–BF2 complex (TMP–BF2) and its 8-methyl (PMP–BF2), 8-ethyl, 2,6,8-trimethyl (HMP–BF2),2,6,-diethyl-8-methyl (PMDEP–BF2), and 2,6-dicarboethoxy-8-ethyl derivatives. Chlorosulfonation converted, 1,3,5,7,8-pentamethylpyrromethene–BF2 complex to its 2,6-disulfonic acid isolated as the lithium, sodium (PMPDS–BF2), potassium, rubidium, cesium, ammonium, and tetramethylammonium disulfonate salts and the methyl disulfonate ester. Sodium 1,3,5,7-tetramethyl-8-ethylpyrromethene-2,6-disulfonate–BF2 complex was obtained from the 8-ethyl derivative of TMP–BF2. Nitration and bromination converted PMP–BF2 to its 2,6-dinitro-(PMDNP–BF2) and 2,6-dibromo- derivatives. The time required for loss of fluorescence by irradiation from a sunlamp showed the following order for P–BF2 compounds (10−3 to 10−4 M) in ethanol: PMPDS–BF2, 7 weeks; PMP–BF2, 5 days; PMDNP–BF2, 72 h; HMP–BF2, 70 h; and PMDEP–BF2, 65 h. Under similar irradiation PMPDS–BF2 in water lost fluorescence after 55 h. The dibromo derivative was inactive, but each of the other pyrromethene–BF2 complexes under flashlamp excitation showed broadband laser activity in the region λ 530–580 nm. In methanol PMPDS–BF2 was six times more resistant to degradation by flashlamp pulses than was observed for Rhodamine-6G (R-6G). An improvement (up to 66%) in the laser power efficiency of PMPDS–BF2 (10−4 M in methanol) in the presence of caffeine (a filter for light <300 nm) was dependent on flashlamp pulse width (2.0 to 7.0 μsec). 相似文献
Summary: Supramolecular interaction of fully methylated hyperbranched polyethylenimines (PEI) with a mesogen‐based carboxylic acid, 5‐(p‐cyanobiphenoxy)pentanoic acid, results in the formation of supramolecular complexes exhibiting thermotropic liquid crystalline (LC) mesophases. In contrast to the common smectic mesophases of most dendritic LC polymers, nematic LC phases were observed. The complexation of PEI and the mesogen units is due to electrostatic interaction between the carboxylate groups and the ammonium end groups of PEI. LC properties were investigated by a combination of differential scanning calorimetry, polarizing light optical microscopy, and X‐ray diffractometry.
Schematic illustration of the supramolecular assembly of CBPA with PEIMe backbone. 相似文献