Dynamic-light-scattering-based sequence-specific recognition of double-stranded DNA with oligonucleotide-functionalized gold nanoparticles

An ultrasensitive and simple dynamic-light-scattering (DLS) assay for the sequence-specific recognition of double-stranded DNA (dsDNA) was developed based on detection of the average diameter change of Au nanoparticle (AuNP) probes modified with oligonucleotides 5'-TTTCTCTTCCTT- CTCTTC-(T)(12)-SH-3' (Oligo 1) and 5'-TTCTTTCTTTTCTTTTTC-(T)(12)- SH-3' (Oligo 2). The target dsDNA was composed of two complementary oligonucleotides: 5'-AAAGAGAAGGAAGAGAAGAAGAAAGAAAAGAAAAAG-3' (Oligo 3) and 3'-TTTCTCTTCCTTCTCTTCTTCTTTCTTTTCTTTTTC-5' (Oligo 4). Hybridization of the two AuNPs-Oligo probes with the target dsDNA induced aggregation of the target dsDNA by forming triplex DNA, which accordingly increased the average diameter. This diameter change could then be detected by DLS. The average diameter was proportional to the target dsDNA concentration over the range from 593 fM to 40 pM, with a detection limit of 593 fM. Moreover, the assay had good sequence specificity for the target dsDNA.     

Gold nanoparticles-based colorimetric investigation of triplex formation under weak alkalic pH environment with the aid of Ag+

A novel colorimetric method for investigating triplex formation between oligonucleotide modified Au nanoparticles (AuNPs) under weak alkalic pH environment is developed based upon the specific recognition property of Ag+ with CGC triads. Oligonucleotide 5'-SH-T12-CTTCTTTCCTTTCTTC-3' (oligo-1) is modified on the surface of AuNPs. Upon addition of oligonucleotide 5'-GAAGAAAGGAAAGAAG-3' (oligo-2), triplex formation between oligo-1 modified AuNPs occurred at pH 8.0 with the aid of Ag+, triggers the aggregation of AuNPs, accompany with the solution color change from red to purple. The melting temperature demonstrates a 31 °C increase for the triplex DNA compose of 10 T?A°T triads and 6 C?G°C triads upon addition of Ag+, the disassociation constant (Kd) between Ag+ and C?G°C triads is 3.6 μM. Moreover, triplex formation between AuNPs depending on Ag+ can be used to recognize Ag+ ion with the naked eye, as well as UV-vis absorption spectroscopy.     

GAE-AFS法分析藏药当佐中总汞与人工胃液中的游离态汞

藏药中的重金属汞已成为藏药安全性评价研究的重点.该文以藏药当佐为研究对象,分别采用湿法消解和人工胃液处理,利用金汞齐富集-冷原子荧光法(GAE-AFS),测定当佐中总汞含量及其在人工胃液中游离态汞含量,并对所建市的方法及仪器的工作条件进行了考察.在选定的测试条件下,仪器系统稳定性良好,HgS的回收率(n=6)为99.5...     

Electromagnetic field produced in high-energy small collision systems within charge density models of nucleons

Recent experiments show that \begin{document}$ \Delta\gamma $\end{document}, an observable designed to detect the chiral magnetic effect (CME), in small collision systems (\begin{document}$ p+A $\end{document}) is similar to that in heavy ion collisions (\begin{document}$ A+A $\end{document}). This introduces a challenge to the existence of the CME because it is believed that no azimuthal correlation exists between the orientation of the magnetic field (\begin{document}$ \Phi_B $\end{document}) and participant plane (\begin{document}$ \Phi_2 $\end{document}) in small collision systems. In this work, we introduce three charge density models to describe the inner charge distributions of protons and neutrons and calculate the electric and magnetic fields produced in small \begin{document}$ p+A $\end{document} collisions at both RHIC and LHC energies. Our results show that the contribution of the single projectile proton is the main contributor to the magnetic field after averaging over all participants. The azimuthal correlation between \begin{document}$ \Phi_B $\end{document} and \begin{document}$ \Phi_2 $\end{document} is small but not vanished. Additionally, owing to the large fluctuation in field strength, the magnetic-field contribution to \begin{document}$ \Delta\gamma $\end{document} may be large.     

Probing top-quark operators with precision electroweak measurements

In the standard model effective field theory, operators involving the top quark are generally difficult to probe and can generate sizable loop contributions to electroweak precision observables measured by past and future lepton colliders. Could the high precision of electroweak measurements compensate for loop suppression and provide competitive reaches on these operators? Would the inclusion of these contributions introduce too many additional parameters for a meaningful global electroweak analysis to be performed? In this paper, we perform a detailed phenomenological study to address these two important questions. Focusing on eight dimension-6 operators that generate anomalous couplings between electroweak gauge bosons and third-generation quarks, we calculate their one loop contributions to \begin{document}$ e^+e^- \to f\bar{f} $\end{document} processes, both on and off the Z-pole, and the \begin{document}$ e^-e^+ \to WW $\end{document} process. A global analysis is performed with these eight operators and those that contribute to the above processes at tree level using measurements at the LEP, SLC, and several low energy experiments. We find that although current electroweak precision measurements are sensitive to the one-loop effects of top-quark operators, it is difficult to separate them from the operators that contribute at tree level, making a global analysis rather challenging. Under further assumptions (for instance, new physics contributes to only third generation quark operators and the S and T parameters), competitive reaches may be obtained in a global fit. Another important finding of our study is that the two operators that generate the dipole interactions of the bottom quark have a significant impact on the Z-pole measurements and should not be omitted. We also discuss the implications of the recently reported W-boson mass measurement at the CDF for our results. Finally, we estimate the reaches of future lepton colliders in probing top-quark operators with precision electroweak measurements.     

Perturbations of May–Leonard system

In this paper we study the quadratic homogeneous perturbations of the 3-dimensional May–Leonard system with α+β=2$\alpha +\beta =2$. It is shown that there are perturbed systems having exactly one or two limit cycles bifurcated from the periodic orbits of May–Leonard system. This is proved by estimating the number of zeros of the first and the second order Melnikov functions.     

The Aggregation Enhanced Photoluminescence of Gold Nanorods in Aqueous Solutions

The photoluminescence (PL) properties of single gold nanorod (AuNR) under one-photon excitation (OPE) have been reported recently. In this work, the PL of AuNRs in aqueous solutions were studied with OPE of 514 or 633 nm to characterize the emissions of transverse and longitudinal surface Plasmon resonance (TSPR and LSPR) bands, because the AuNRs aqueous solution was frequently used in bio-medical applications. We found that under 514 nm OPE the TSPR emissions of four groups of AuNRs with different aspect ratios in aqueous solutions were all strong dominating the PL emission with the quantum yield (QY) of 10^?4, which is at least three orders of magnitude higher than that of single AuNR. We further found that the aggregate was the basic form of AuNRs in aqueous solution and living cells, measured by the elastic light scattering and transmission electron microscopy measurements. The Plasmon coupling particularly the TSPR coupling between the neighbored AuNRs in aggregates enhanced the PL and increased the QY, because the conjugation of the rod side to side was a main aggregate mode. Under 633 nm OPE, only LSPR emissions of AuNRs aqueous solutions occurred with the QY level of 10^?5 which is very similar to that of singe AuNR, because of the negligible LSPR coupling.     

Three-dimensional nanographene based on triptycene for detection of nitroaromatic explosives

3D nanographene 1, with three HBC units arraying in the 3D triptycene scaffold, displayed strong intrinsic fluorescence properties and could be used as a detector for nitroaromatic explosives such as TNP with quenching efficiency K_sv of 1.8 × 10⁴ M⁻¹ and sensitivity of 2.4 ng/mm², respectively.     

樟脑基苯甲酰胺类化合物的合成及生物活性研究

以天然樟脑为原料,经中间体樟脑肟和樟脑胺,以及樟脑胺与取代苯甲酰氯的N-酰化反应,合成得到9个樟脑基苯甲酰胺类化合物(3a～3i)。初步探索了合成条件,并通过FT IR、1H NMR、13C NMR、ESIMS和元素分析等多种手段对目标产物进行结构表征。生物活性测试表明,在50mg/L浓度下,所合成的化合物对5种植物病原菌均有一定的抑制作用,其中化合物3c(R=2-Cl)和3i(R=4-F)对苹果轮纹病菌的抑制率分别达97.5%和96.4%,化合物3i对小麦赤霉病菌的抑制率为95.7%。在100mg/L浓度下,化合物对油菜的胚根生长均显示良好的抑制活性,其中化合物3b(R=4-Cl)的抑制率达97.4%。