High-resolution Hadamard transform microscope fluorescence imaging: quantifying the DNA content in single cells

This study presents a novel miniaturized Hadamard transform fluorescence imaging microscope, by combining a conventional fluorescence microscope with Hadamard transform multiplexing encoding using a one-dimensional movable mask to realize spatial resolution and a linear CCD for multichannel detection. The microscope can provide high-resolution automatically-generated 0–255 gray level HT images for morphological analysis and visualization of a single cell, and normalized HT images for cellular quantitative measurements. The microscopes imaging capability was applied to measure the DNA content in human lymphocyte, chicken erythrocyte and eel erythrocyte, and a comparative study was performed. The results show that the calibrated DNA content in a chicken erythrocyte is 2.32 pg when human lymphocyte is used as the standard, and eel erythrocyte may be a potentially reliable and novel standard for determining DNA contents in other species because it has a stable DNA value of 2.06 pg, with a CV of 4.3% when 20 eel erythrocytes are measured. The results also demonstrate that the HT imaging microscope should be valuable in the fields of medicine and cell biology.     

Combine Harvester Bearing Fault-Diagnosis Method Based on SDAE-RCmvMSE

In the fault monitoring of rolling bearings, there is always loud noise, leading to poor signal stationariness. How to accurately and efficiently identify the fault type of rolling bearings is a challenge. Based on multivariate multiscale sample entropy (mvMSE), this paper introduces the refined composite mvMSE (RCmvMSE) into the fault extraction of the rolling bearing. A rolling bearing fault-diagnosis method based on stacked auto encoder and RCmvMSE (SDAE-RCmvMSE) is proposed. In the actual environment, the fault-diagnosis method use the multichannel vibration signals of the bearing as the input of stacked denoising autoencoders (SDAEs) to filter the noise of the vibration signals. The features of denoise signals are extracted by RCmvMSE and the rolling bearing operation-state diagnosis is completed with a support-vector machine (SVM) model. The results show that in the original test data, the accuracy rates of SDAE-RCmvMSE, RCmvMSE, and commonplace features of vibration signals combined with SVM (CFVS-SVM) methods are 99.5%, 100%, and 96% respectively. In the data with noise, the accuracy rates of RCmvMSE and CFVS-SVM are 97.75% and 93.08%, respectively, but the accuracy of SDAE-RCmvMSE is still 100%.     

规模生猪养殖液态生物质资源循环开发仿真分析

对银河杜仲基于沼气工程的液态生物质资源的循环开发进行仿真分析。根据历史数据和公司五年、十年发展规划,使用基于反馈调控参数的入树逐步建模法,构建7棵入树仿真模型;通过仿真定量揭示公司规划实施过程中将产生大量废弃物类生物质,沼气工程对废弃物开发产生的沼液和沼气中含大量的生物质能;为此,基于仿真结果及系统反馈结构,提出促进消除环境与猪肉产品双污染,实现绿色安全猪肉生产和改善环境双重目标的三条管理对策,并进行初步实施。     

基于非平衡光纤干涉仪的窄线宽激光光源跳模实时测试方法

窄线宽激光器随机发生的跳模现象,是影响光学系统稳定的重要因素.激光器线宽窄往往意味着较长的谐振腔和较小的模间隔,从而给跳模的监测带来了难度.本文提出一种基于非平衡光纤干涉仪的窄线宽光源跳模测试方法,该方法将激光器跳模的频率变化转变为干涉仪的相位变化,并通过相位产生载波(PGC)调制解调技术来检测相位信息,从而实现对跳模过程的监测.该方法灵敏度高,能够长时间连续监控,测试效果优于Fabry-Pérot干涉仪,还可在kHz量级上测量窄线宽激光器的线宽,为单纵模窄线宽激光器研究提供有效的测试手段.     

X射线CCD标定及模拟

 在北京同步辐射光源4B7B实验站上,采用透射光栅配X射线电荷耦合元件(CCD)的方法对单色光进行了检验,在优化高次谐波抑制方法后,开展了X射线CCD的灵敏度标定实验研究;在标定数据处理过程中,提出了等效曝光时间的概念,修正了快门开合造成的曝光时间误差。标定实验获得了100~1 500 eV能区CCD的灵敏度,补充了300~600 eV能区CCD灵敏度标定数据,验证了简化模型的正确性。     

Al2O3薄膜/纳米Ag颗粒复合结构的光吸收谱及增强Raman散射光谱研究

Al₂O₃介质薄膜与纳米Ag颗粒构成的复合结构,被应用于表面增强Raman散射探测实验中,其中Al₂O₃介质薄膜对纳米Ag颗粒的吸收谱及增强Raman散射光谱的影响被特别关注.该复合结构的光学特性表征出纳米Ag颗粒的偶极振荡特性.从光吸收谱中可以看到,其共振吸收谱随Al₂O₃介质薄膜厚度增加而在整个谱域上发生红移,表明纳米Ag颗粒的周围介电常数随Al₂O₃介质薄膜厚度的增加而增大.采用罗丹明6G作为探针原子,6个Raman特征峰的平均增益值作为表征表面增强Raman散射衬底增益程度的量度.实验结果表明,Al₂O₃介质薄膜层的引入提高了纳米Ag颗粒的衬底介电常数,并引起了散射共振的增强,从而使表面增强Raman散射强度提高.     

气溶胶单粒子粒径的实时测量方法研究

介绍了近期研制的一台实时测量大气气溶胶单粒子粒径和化学成分的仪器在如何测量气溶胶单粒子粒径方面的测量原理及特点，并结合激光解吸附电离飞行时间质谱技术，利用该仪器对木屑燃烧产生的烟气气溶胶粒子的实时测量结果. 关键词： 气溶胶粒子 空气动力学粒径测量方法 激光解吸附电离 飞行时间质谱     

Multiple teleportation via partially entangled GHZ state

Quantum teleportation is important for quantum communication. We propose a protocol that uses a partially entangled Greenberger–Horne–Zeilinger (GHZ) state for single hop teleportation. Quantum teleportation will succeed if the sender makes a Bell state measurement, and the receiver performs the Hadamard gate operation, applies appropriate Pauli operators, introduces an auxiliary particle, and applies the corresponding unitary matrix to recover the transmitted state.We also present a protocol to realize multiple teleportation of partially entangled GHZ state without an auxiliary particle. We show that the success probability of the teleportation is always 0 when the number of teleportations is odd. In order to improve the success probability of a multihop, we introduce the method used in our single hop teleportation, thus proposing a multiple teleportation protocol using auxiliary particles and a unitary matrix. The final success probability is shown to be improved significantly for the method without auxiliary particles for both an odd or even number of teleportations.     

3－苯氧甲基－6－（2，4－二氟苯基）－7H-1,2,4-三唑并[3,4-b][1,3,4]噻二嗪的合成与晶体结构研究

由苯氧乙酸出发，经多步反应，制得3-苯氧甲基－4－氨基－5－巯基－1，2， 4－三唑，它与2-氯－2'，4'－二氟苯乙酮进行环化反应，生成3-苯氧甲基－6－（ 2,4-二氟苯基）－7H-1,2,4-三唑并[3,4-b][1,3,4]噻二嗪，通过元素分析、红外 光谱、核磁共振氢谱与碳谱、质谱进行表征，并利用单晶X射线衍射法测定其结构 。晶体属单斜晶系、P2_(1/c)空间群，a = 1.339(3) nm, b = 1.4683(4) nm, c = 0.8606(2) nm, β = 108.49(2)°, Z = 4, F(000) = 736, R_1 = 0.0509。还 对均三唑并噻二唑两类稠杂环的晶体结构作了比较。     

Synthesis of Highly Uniform Molybdenum–Glycerate Spheres and Their Conversion into Hierarchical MoS2 Hollow Nanospheres for Lithium‐Ion Batteries

Highly uniform Mo–glycerate solid spheres are synthesized for the first time through a solvothermal process. The size of these Mo–glycerate spheres can be easily controlled in the range of 400–1000 nm by varying the water content in the mixed solvent. As a precursor, these Mo–glycerate solid spheres can be converted into hierarchical MoS₂ hollow nanospheres through a subsequent sulfidation reaction. Owing to the unique ultrathin subunits and hollow interior, the as‐prepared MoS₂ hollow nanospheres exhibit appealing performance as the anode material for lithium‐ion batteries. Impressively, these hierarchical structures deliver a high capacity of about 1100 mAh g^?1 at 0.5 A g^?1 with good rate retention and long cycle life.