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101.
Miguel Alcaide Lidia Lpez Alessandro Tanferna Julio Blas Fabrizio Sergio Fernando Hiraldo 《Electrophoresis》2010,31(8):1353-1356
Major histocompatibility complex (MHC) genotyping still remains one of the most challenging issues for evolutionary ecologists. To date, none of the proposed methods have proven to be perfect, and all provide both important pros and cons. Although denaturing capillary electrophoresis has become a popular alternative, allele identification commonly relies upon conformational polymorphisms of two single‐stranded DNA molecules at the most. Using the MHC class II (β chain, exon 2) of the black kite (Aves: Accipitridae) as our model system, we show that the simultaneous analysis of overlapping PCR amplicons from the same target region substantially enhances allele discrimination. To cover this aim, we designed a multiplex PCR capable to generate four differentially sized and labeled amplicons from the same allele. Informative peaks to assist allele calling then fourfold those generated by the analysis of single PCR amplicons. Our approach proved successful to differentiate all the alleles (N=13) isolated from eight unrelated birds at a single optimal run temperature and electrophoretic conditions. In particular, we emphasize that this approach may constitute a straightforward and cost‐effective alternative for the genotyping of single or duplicated MHC genes displaying low to moderate sets of divergent alleles. 相似文献
102.
Boichenko Alexander P. Makhno Iryna V. Renkevich Anton Yu. Loginova Lidia P. 《平面色谱法杂志一现代薄层色谱法》2011,24(6):463-469
JPC – Journal of Planar Chromatography – Modern TLC - The physical-chemical characteristics (surface tension and viscosity) of micellar mobile phases based on the cationic surfactant... 相似文献
103.
Annalisa Bertoli Lidia Fanfoni Fulvia Felluga Giuliana Pitacco Ennio Valentin 《Tetrahedron: Asymmetry》2009,20(19):2305-2310
Three α-methylene-γ-carbomethoxy-γ-butyrolactams (methyl α-methylene-pyroglutamates) 11, 12 and 13, differing in the substitution at the heterocyclic nitrogen, as well as the structurally related γ-lactones 14 and 15 were synthesised and resolved enzymatically by hydrolysis of their ester function, mediated by commercially available hydrolytic enzymes. In particular, the α-chymotrypsin proved to be active to all the substrates examined, displaying a different degree of activity and enantioselectivity, this latter increasing significantly towards the substrate with an aromatic substituent at the nitrogen. 相似文献
104.
Carlos Camacho‐Camacho Irma Rojas‐Oviedo M. Angeles Paz‐Sandoval Jorge Cárdenas Alfredo Toscano Marcel Gielen Lidia Barrón Sosa Francisco Sánchez Bártez Isabel Gracia‐Mora 《应用有机金属化学》2008,22(3):171-176
The synthesis, characterization and cytotoxic properties in vitro of tri‐n‐butyltin 1‐(4‐chlorobenzoyl)‐5‐methoxy‐2‐methyl‐1H‐indole‐3‐acetate ( 1 ), tri‐phenyltin 1‐(4‐chlorobenzoyl)‐5‐methoxy‐2‐methyl‐1H‐indole‐3‐acetate ( 2 ), tetra‐n‐butyltin[bis‐1‐(4‐chlorobenzoyl)‐5‐methoxy‐2‐methyl‐1H‐indole‐3‐acetato]distannoxane ( 3 ) and di‐n‐butyltin bis‐1‐(4‐chlorobenzoyl)‐5‐methoxy‐2‐methyl‐1H‐indole‐3‐acetate ( 4 ) are described. These compounds have been characterized by 1H, 13C and 119Sn NMR spectroscopy in solution and 119Sn NMR in the solid state, infrared spectroscopy, elemental analysis and X‐ray diffraction for compound 1 . The growth inhibition effects of compounds 1–4 against the lung adenocarcinoma cell line SK‐LU‐1 as well as the cervical cancer cell line HeLa were determined. Compounds 1 and 2 exhibit cytotoxic activity, whereas compounds 3 and 4 are inactive. Copyright © 2008 John Wiley & Sons, Ltd. 相似文献
105.
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In this communication, we revise some aspects of the [ideal gas/Van der Waals fluid] partition, derived in an earlier publication. The general character of the conclusions concerning the dependence of the partial molar free energy of solution, deltaGs(n), on the chain length, n, of linear solute molecules is shown through the relationship with more general partition formulations. Simultaneously, the correction of an error in the expression of the retention time dependence on the phase ratio of the chromatographic column, beta, is carried out. The misleading source of this error was redundant accounting in the solute translational contribution to deltaGs(n). 相似文献
107.
108.
D A Ruggirello P K Noonan M A Gonzalez P Ho J G Wagner 《Journal of chromatography. A》1989,487(1):73-80
A highly sensitive gas chromatographic assay is described for the simultaneous determination of gallopamil, a calcium channel blocking agent, and its major metabolite, norgallopamil. A multi-step extraction procedure is employed followed by on-column capillary gas chromatographic analysis using nitrogen-selective detection. Acetylation of norgallopamil is performed to enable accurate quantification of the metabolite. Linearity was achieved over the range 1-50 ng/ml for both analytes. Assay specificity, precision and accuracy were investigated. 相似文献
109.
Pedro J. Catuogno Sebastian E. Ferrando Alfredo L. Gonzalez 《Journal of Fourier Analysis and Applications》2008,14(5-6):712-743
H-systems are those orthonormal systems which allow computation of conditional expectations via a Fourier expansion. These systems provide natural approximations to continuous stochastic processes and we indicate how they could be used to perform lossy compression on a set of given signals. More specifically, we show how to construct, for a given random variable, an adaptive martingale approximation by means of generalized Haar functions. We also indicate how the construction can be extended to a given random vector. A generalized multiresolution analysis algorithm is also described and numerical examples are provided. 相似文献
110.