白血病骨髓移植病人全血微量元素铜的变化及其意义

为了研究白血病骨髓移植病人全血微量元素铜的变化及其意义 ,用原子吸收光谱法检测了正常对照组与白血病骨髓移植患者预处理前及移植后骨髓空虚期全血微量元素铜的含量。结果表明 ,正常对照组全血铜浓度与白血病骨髓移植患者预处理前全血铜浓度差异无显著性 (P >0 0 5 ) ,而与骨髓空虚期全血铜浓度有显著性差异 (P <0 0 5 ) ,说明白血病缓解后血铜接近正常对照组水平 ,而白血病骨髓移植患者预处理后骨髓空虚期全血铜含量高于正常对照组 ,说明预处理影响微量元素铜的代谢 ,本文就其影响机理进行了研究     

SrZnO_2∶Eu~(3+),Li~+长波紫外激发红光荧光体的合成及发光性能研究

采用高温固相法合成了一种长波紫外激发的SrZnO2∶Eu3+,Li+发光材料,用X射线衍射谱、荧光光谱对样品进行了表征。结果表明,Eu3+离子在SrZnO2基质中主要占据Sr2+离子不对称性格位,发射来源于5D0→7F2612 nm为主的红光。加入电荷补偿剂Li+离子能显著提高发光强度,350~400 nm内的激发峰也有明显提高,同时观察到来自Eu3+离子高能级5D1→7FJ(J=0~2)的跃迁发射,并对其产生机制进行了初步探讨。实验结果表明,SrZnO2∶Eu3+,Li+是一种有发展前途的长波紫外激发红光荧光体。     

In-channel indirect amperometric detection of nonelectroactive anions for electrophoresis on a poly(dimethylsiloxane) microchip

In the present paper, we describe a microfluidics-based sensing system for nonelectroactive anions under negative separation electric field by mounting a single carbon fiber disk working electrode (WE) in the end part of a poly(dimethylsiloxane) microchannel. In contrast to work in a positive separation electric field described in our previous paper (Anal. Chem. 2004, 76, 6902-6907), here the electrochemical reduction reaction at the WE is not coupled with the separation high-voltage (HV) system, whereas the electrochemical oxidation reaction at the WE is coupled with the separation HV system. The electroactive indicator is the carbon fiber WE itself but not dissolved oxygen. This provides a convenient and sensitive means for the determination of nonelectroactive anions by amperometry. The influences of separation voltage, detection potential, and the distance between the WE and the separation channel outlet on the response of the detector have been investigated. The present detection mode is successfully used to electrochemically detect F-, Cl-, SO4(2-), CH3COO-, H2PO4-. Based on the preliminary results, a detection limit of 2 microM and a dynamic range up to three orders of magnitude for Cl- could be achieved.     

The Study of Anti-tumor Activity of Trichosanthin by Cyclic Voltammogram

IthasbeenreportedthatTrichosanthi11(TCS),isolatedfromrkiI-ilolt)ii,hasanti-tumorI-4andanti-vira1activities5-'.Someanti-tun1orremedies,whicharedesignedfromTCS,arebeingtestedanddeveloped'-.'.StudyingthebioIogicalphenomenonbyelectrochemicalmethodsisanewhotresearchspotemerginginrecentyears".Manyresultsshowedthatnotonlytheenzymesystemsuchassuccinatedehydrogenasedisplayedthediode-likebehaviorduringtheelectrontransferprocess",butalsothehigh1yorganizedorganeIle,forexample,mitochondria,andevenintac…     

Determination of the Enantiomeric Purity of l-Carnitine and Acetyl-l-Carnitine by Chiral Liquid Chromatography

A chiral liquid chromatographic method for determination of the enantiomeric purity of both l-carnitine and acetyl-l-carnitine is described. Separation of the enantiomers of dl-carnitine and acetyl-dl-carnitine was achieved on a commercial chiral column (Chiralcel OD-R) after derivatization with (alpha-bromo)methyl phenyl ketone. Introduction of this lipophilic UV chromophoric group to the carnitine and acetylcarnitine molecules improved their retention, resolution, and UV detection. The mobile phase was 74:26 (v/v) 0.5 mol L^-1 sodium perchlorate–acetonitrile, pH 3.8, and the flow rate was 0.4 mL min^-1. Detection was performed at 235 nm. The method is selective and reliable for determination of the enantiomeric purity of bulk drug substances l-carnitine and acetyl-l-carnitine.     

苏丹Ⅰ与牛血清白蛋白的相互作用特征

利用荧光技术研究了在人体生理酸度条件下,苏丹Ⅰ与牛血清白蛋白相互作用,发现苏丹Ⅰ对牛血清白蛋白有较强的荧光猝灭作用,用Stern-Volmer和Line Weaver-Burk方程处理荧光猝灭数据,得到了反应的结合常数、结合热力学性质和结合位点等参数。根据热力学常数确定了该药物与血清白蛋白之间的作用力类型,在此基础上依据F rster非辐射能量转移机理探讨了苏丹Ⅰ与BSA相互结合时其供体-受体间的距离。     

同时测定食用植物油中抗氧剂叔丁基对羟基茴香醚和2，6－二叔丁基对甲酚的方法

报道了用高效液用色谱法同时测定食用植物油中的抗氧剂叔丁基对羟基茴香醚（ＢＨＡ）和２，６－二叔丁基对甲酚（ＢＨＴ）的实验方法。采用ＺｏｒｂａｘＯＤＳＣ_（１８）色谱住，甲醇：水（９０：１０，Ｖ／Ｖ）作流动相，检测波长为２４５ｎｍ，流速为１ｍＬ／ｍｉｎ。检测下限分别为１５ｎｇ（ＢＨＡ），９０ｎｇ（ＢＨＴ），回收率分别是９６．８％±９．８％（ＢＨＡ），９９．０％±３．２％（ＢＨＴ）。方法快速、准确，灵敏度高。     

Electrothermal volatilization of aluminum as 1-phenyl-3-methyl-4-benzoylpyrazolone[5] chelate for gaseous sample introduction in ICP-AES

Based on gaseous compound introduction as 1-phenyl-3-methyl-4-benzoylpyrazolone[5](PMBP) chelate of aluminum by electrothermal vaporization in ICP-AES, a method for determination of trace aluminum was developed. Trace aluminum was vaporized at temperature of 1000 degrees C, and the vaporization behavior of aluminum chelate was detailedly investigated. Under the optimum conditions, the detection limit of aluminum was 0.6 ng ml(-1), and the relative standard deviation for 0.1 mug ml(-1) aluminum was 4.7% (n=8). The proposed method was applied to the determination of trace aluminum in rice flour reference materials, and the results well agreed with the reference values.     

LaPO4纳米微粒的制备及表征

Surface-modified LaPO4 nanoparticales were synthesized in the mixture solvent of water-ethanol with the surface modification age of dialkylphosphate. The conditions of synthesis and characterization were discussed. The results indicated that nanoparticle core of LaPO4 and surface-modified layer were formed. The surface-modified nanoparticles of LaPO4 can be dispersed in organic solvent and has better lubricating properties.     

液相色谱专家系统中柱系统推荐规则的建立和验证

把分子结构和分子、离子静电作用力作为影响溶质保留值和选择性的基本点,提出了液相色谱专家系统柱系统推荐的基本规则,由此可以从溶质所包含的基团或官能团的信息进行色谱柱系统推荐。为了验证这些规则的正确性,应用几类常用的或典型的样品从分子结构通道进行液相色谱柱系统推荐,获得满意的结果。