Mediated electron transfer of cellobiose dehydrogenase and glucose oxidase at osmium polymer-modified nanoporous gold electrodes

Nanoporous and planar gold electrodes were utilised as supports for the redox enzymes Aspergillus niger glucose oxidase (GOx) and Corynascus thermophilus cellobiose dehydrogenase (CtCDH). Electrodes modified with hydrogels containing enzyme, Os-redox polymers and the cross-linking agent poly(ethylene glycol)diglycidyl ether were used as biosensors for the determination of glucose and lactose. Limits of detection of 6.0 (±0.4), 16.0 (±0.1) and 2.0 (±0.1) μM were obtained for CtCDH-modified lactose and glucose biosensors and GOx-modified glucose biosensors, respectively, at nanoporous gold electrodes. Biofuel cells composed of GOx- and CtCDH-modified gold electrodes were utilised as anodes, together with Myrothecium verrucaria bilirubin oxidase (MvBOD) or Melanocarpus albomyces laccase as cathodes, in biofuel cells. A maximum power density of 41 μW/cm² was obtained for a CtCDH/MvBOD biofuel cell in 5 mM lactose and O₂-saturated buffer (pH 7.4, 0.1 M phosphate, 150 mM NaCl).     

Computer simulation of the trajectories of large water jets

A three-dimensional computer simulation of the motion of a water jet is described which includes the effects of wind from any direction. The simulation is useful in the design of fire-fighting systems, particularly those used in offshore situations. The equations of motion are presented in vector form and the problem of the fluid dynamic drag variation is discussed. Semi-empirical approximations for the drag components along and across the jet are presented which involve four unknown constants. These are reduced to three by using previous data on the efficiency of vertical jets. To fix the remaining constants, information was available from a series of large jets tests carried out to prove an offshore fire fighting system. In these tests different nozzle shapes were tried and, using the best of these shapes, a large number of trajectories were measured photographically. These were used to fix the simulation drag constants and good agreement is shown between measurements and predictions. The simulation enables the effects of flow-rate, pressure, nozzle size, elevation and wind strength to be evaluated in the system design     

New high-energy luminescence bands from Co2+ in ZnSe

Three sharp absorption features in the energy range 2.36–2.55 eV have been detected in the transmission spectrum of Co-diffused ZnSe, and a number of luminescence transitions originating from the lowest of these states at 2.361 eV have been observed. Photoluminescence excitation spectra prove that these are high energy excited states of the Co²⁺_Zn impurity, a conclusion confirmed by comparison of measured and predicted luminescence energies. This represents the first identification of luminescence branching from a higher excited state of a transition metal ion in any semiconductor. The sharp, weakly phonon-coupled transitions involve either intra-impurity excitation or transitions from the impurity to localised states split off from a minimum in the conduction band. The implications of these observations for the mechanism of host-impurity energy transfer and for the nature of the excited state wavefunctions are discussed.     

Patterning of PLZT and PSZT thin films by excimer laser

The patterning of lanthanum-doped lead zirconate titanate (PLZT) and strontium-doped lead zirconate titanate (PSZT) thin films has been examined using a 5-ns pulsed excimer laser. Both types of film were deposited by rf magnetron sputtering with in situ heating and a controlled cooling rate in order to obtain the perovskite-structured films. The depth of laser ablation in both PSZT and PLZT films showed a logarithmic dependence on fluence. The ablation rate of PLZT films was slightly higher than that of PSZT films over the range of fluence (10–150 J/cm²) and increased linearly with number of pulses. The threshold fluence required to initiate ablation was ∼ 1.25 J/cm² for PLZT and ∼ 1.87 J/cm² for PSZT films. Individual squares were patterned with areas ranging from 10×10 μm² up to 30×30 μm² using single and multiple pulses. The morphology of the etched surfaces comprised globules which had diameters of 200–250 nm in PLZT and 1400 nm in PSZT films. The diameter of the globules has been shown to increase with fluence until reaching an approximately constant size at ≤ 20 J/cm² in both types of film. The composition of the films following ablation has been compared using X-ray photoelectron spectroscopy and energy-dispersive X-ray spectroscopy. PACS 79.20.Ds; 82.80.Pv; 82.80.Ej     

Skew Boolean algebras derived from generalized Boolean algebras

Every skew Boolean algebra S has a maximal generalized Boolean algebra image given by S/ where is the Green’s relation defined initially on semigroups. In this paper we study skew Boolean algebras constructed from generalized Boolean algebras B by a twisted product construction for which . In particular we study the congruence lattice of with an eye to viewing as a minimal skew Boolean cover of B. This construction is the object part of a functor from the category GB of generalized Boolean algebras to the category LSB of left-handed skew Boolean algebras. Thus we also look at its left adjoint functor . This paper was written while the second author was a Visiting Professor in the Department of Education at the University of Cagliari. The facilities and assistance provided by the University and by the Department are gratefully acknowledged.     

A 350GHz Radial-Probe SIS Mixer for Astronomical Imaging Arrays

We have developed a 330-370GHz SIS mixer for small-format, heterodyne, astronomical imaging arrays. Fixed-tuned broadband operation is achieved by means of a superconducting radial waveguide probe. A horn-reflector antenna provides high-efficiency optical coupling. Using a variable-temperature cryogenic noise source, we measured a DSB system noise temperature of 32±1K. The mixer contributes 3±3K, supporting the theoretically-predicted result that the noise temperature of a DSB mixer can be less than h/2 (8.6K)     

Simultaneous attainment of high electron and ion temperatures in discharges with internal transport barriers in ASDEX upgrade

Internal transport barriers have been demonstrated to exist also under conditions with T(e) approximately T(i) approximately 10 keV and predominant electron heating of the tokamak core region. Central electron cyclotron heating was added to neutral beam injection-heated ASDEX Upgrade discharges with a preexisting internal transport barrier, established through programmed current ramping leading to shear reversal. Compared to a reference internal transport barrier discharge without electron cyclotron resonance heating, the electron heat conductivity in the barrier region was found not to increase, in spite of a fivefold increase in electron heat flux, and also angular momentum and ion energy transport did not deteriorate.     

Skew lattices in rings

Presented by Boris M. Schein.