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91.
Using enalapril maleate as a test case, the ability of ambient mass spectrometry, namely, via easy ambient sonic‐spray ionization mass spectrometry (EASI‐MS), to perform direct monitoring of drug degradation has been tested. Two manufacturing processes were investigated (direct compression and wet granulation), and the formation of degradation products was measured via both EASI‐MS and high‐performance liquid chromatography with ultraviolet detection for a total period of 18 months. Both techniques provide comparable results, which indicate that direct analysis by ambient mass spectrometric techniques presents a viable alternative for drug degradation monitoring with superior simplicity, throughput, and reliability (no sample manipulation), and comparable quantitative results. In terms of qualitative monitoring, the full mass spectra with intact species provided by EASI‐MS allow for comprehensive monitoring of known and unknown (or unexpected) degradation products. Copyright © 2011 John Wiley & Sons, Ltd. 相似文献
92.
Popularity of new psychoactive substances, known as legal highs or herbal highs, is continuously growing. These products are
typically sold via internet and in so-called head shops. The aim of this study was to identify active ingredients of herbal
highs and to compare their chemical composition. Twenty-nine various products seized by the police in one of the “head shops”
were analysed. Herbal mixtures (0.2 g) were prepared by ultrasonic-assisted extraction with 2.0 ml of ethanol for 2 h. The
extracts were analysed by gas chromatography coupled to mass spectrometry (GC/MS). The main active compounds of the herbal
mixtures were synthetic cannabinoids: JWH-018, JWH-073 and cannabicyclohexanol (CP-47,497-C8-homolog). Their content differed
between the products; some contained only one cannabinoid whereas the others contained two or more. Cluster analysis and principal
component analysis revealed that chemical composition of many products was very similar. The similarity was connected with
their flavour and not the common name. This statement was true for the synthetic cannabinoids, other potential agonists of
cannabinoid receptors (amides of fatty acids) and ingredients of natural origin and confirms that herbal highs are a threat
to human health because the purchaser has no information on their real composition. 相似文献
93.
In this paper we present two new algorithmic variants to compute the Neville elimination, with and without pivoting, which improve data locality and cast most of the computations in terms of high-performance Level 3 BLAS. The experimental evaluation on a state-of-the-art multi-core processor demonstrates that the new blocked algorithms exhibit a much higher degree of concurrency and better cache usage, yielding higher performance while offering numerical accuracy akin to that of the traditional columnwise variant in most cases. 相似文献
94.
Rangel TB Assreuy AM Pires Ade F Carvalho AU Benevides RG Simões Rda C Silva HC Bezerra MJ Nascimento AS Nascimento KS Nagano CS Sampaio AH Delatorre P Rocha BA Fernandes PM Cavada BS 《Molecules (Basel, Switzerland)》2011,16(6):5087-5103
DwL, a lectin extracted from the seeds of Dioclea wilsonii, is a metalloprotein with strong agglutinating activity against rabbit and ABO erythrocytes, inhibited by glucose and mannose. DwL was purified by affinity chromatography on a Sephadex G-50 column and ion exchange chromatography on a HiTrap SP XL column. SDS-PAGE revealed three electrophoretic bands corresponding to the α (25,634 ± 2 Da), β (12,873 ± 2 Da) and γ (12,779 ± 2 Da) chains. Protein sequencing was done by Tandem Mass Spectrometry. The primary sequence featured 237 amino acids and was highly homologous to other reported Diocleinae lectins. A complete X-ray dataset was collected at 2.0 ? for X-Man-complexed DWL crystals produced by the vapor diffusion method. The crystals were orthorhombic and belonged to the space group I222, with the unit-cell parameters a = 59.6, b = 67.9 and c = 109.0 ?. DWL differed in potency from other ConA-like lectins and was found to induce neutrophil migration in rats, making it particularly useful in structural/functional studies of this class of proteins. 相似文献
95.
Corma A Boronat M Climent MJ Iborra S Montón R Sabater MJ 《Physical chemistry chemical physics : PCCP》2011,13(38):17255-17261
A series of bifunctional organic catalysts containing acid and basic sites with ionic liquid characteristics have been prepared and their catalytic activity and reaction coordinate for aldol and Knoevenagel condensations have been compared. While the only factor controlling catalyst activity for the Knoevenagel condensation was the distance between the acid and base sites, the spatial orientation of the organocatalyst is also key to achieve high activity and selectivity in the Claisen-Schmidt condensation. Mechanistic studies based on theoretical DFT calculations show that the acid-base bifunctional organocatalyst follows a mechanism inspired in natural aldolases for the synthesis of trans-chalcones, being able to produce a large variety of these compounds of industrial interest. The combination of the acid-base pairs within the proper geometry and the ionic liquid nature makes this catalyst active, selective and recyclable. 相似文献
96.
Numerical Algorithms - A method for solving least squares problems (A ? Bi)x = b whose coefficient matrices have generalized Kronecker product structure is presented. It is based on the... 相似文献
97.
A contracted basis set of triple zeta (TZ) valence quality for the atoms from K to Kr was constructed from fully-optimized Gaussian basis sets generated in this work. Gaussian polarization functions (d, f, and g symmetries), which were optimized at the second-order Mφller–Plesset level, were added to the TZ set. This extends earlier work on segmented contracted TZ basis set for atoms H-Ar. This set along with the BP86 non-hybrid and B3LYP hybrid functionals were used to calculate geometric parameters, dissociation energy, harmonic vibrational frequency, and electric dipole moment of a sample of molecules and, then, comparison with results obtained with other basis sets and with experimental data reported in the literature is done. CCSD(T) atomic excitation energies and bond lengths, dissociation energies, and harmonic vibrational frequencies of some diatomics were also evaluated. Using density functional theory and gauge-including atomic orbitals, 57Fe and 77Se nuclear magnetic resonance chemical shifts in Fe(C5H5)2, H2Se, (CH3)SeH, CSe2, SeCO, H2CSe, and SeF6 were calculated. Comparison with theoretical and experimental values previously published in the literature was done. It is verified that in general these results give good agreement with experimental and benchmark values. 相似文献
98.
A bead-injection system is proposed for total mercury determination in river-water samples. The procedure is based on the introduction of a defined quantity of a resin suspension in the flow system. The selected beads are packed inside of a flow cell and the formed resin mini-column constitutes the optical path. The sample volume is then selected, and its passage by the mini-column allows retention of the mercury ions on the surfaces of the beads. The introduction of a spectrophotometric reagent in the flow system leads to the formation of a colored Hg-dithizone complex on the surface of the bead, which is spectrophotometricaly monitored. The spent beads are directed to waste, allowing the system to become ready to process another sample. The proposed system handles about 20 measurements per hour, consuming 1000 microl of the sample, 1 mg of Chelex 100 resin and 1.25 microg of Dithizone per determination. When 1000 microl of the sample is injected, a linear analytical curve is obtained (A = 0.0052[Hg] + 0.1028, from 0 up to 30 microg l(-1), R2 = 0.995); the detection limit is estimated to be 0.9 microg l(-1). The results are precise, r.s.d. < 9%; spiked sample recoveries within 91.2 and 109% are found. 相似文献
99.
Jaime Rodríguez André Ferraz Raquel F. P. Nogueira Irene Ferrer Elisa Esposito Nelson Durán 《Applied biochemistry and biotechnology》1997,62(2-3):233-242
The lignin biodegradation process has an important role in the carbon cycle of the biosphere. The study of this natural process
has developed mainly with the use of basidiomycetes in laboratory investigations. This has been a logical approach since most
of the microorganisms involved in lignocellulosic degradation belong to this class of fungi. However, other microorganisms
such as ascomycetes and also some bacteria, are involved in the lignin decaying process. This work focuses on lignin biodegradation
by a microorganism belonging to the ascomycete class,Chrysonilia sitophila. Lignin peroxidase production and characterization, mechanisms of lignin degradation (lignin model compounds and lignin in
wood matrix) and biosynthesis of veratryl alcohol are outstanding. Applications of C.sitophila for effluent treatment, wood biodegradation and single-cell protein production are also discussed. 相似文献
100.