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61.
Laxman?Mainali Theodore?G.?Camenisch James?S.?Hyde Witold?K.?SubczynskiEmail author 《Applied magnetic resonance》2017,48(11-12):1355-1373
The presence of integral membrane proteins induces the formation of distinct domains in the lipid bilayer portion of biological membranes. Qualitative application of both continuous wave (CW) and saturation recovery (SR) electron paramagnetic resonance (EPR) spin-labeling methods allowed discrimination of the bulk, boundary, and trapped lipid domains. A recently developed method, which is based on the CW EPR spectra of phospholipid (PL) and cholesterol (Chol) analog spin labels, allows evaluation of the relative amount of PLs (% of total PLs) in the boundary plus trapped lipid domain and the relative amount of Chol (% of total Chol) in the trapped lipid domain (Raguz et al. Exp Eye Res 140:179–186, 24). Here, a new method is presented that, based on SR EPR spin-labeling, allows quantitative evaluation of the relative amounts of PLs and Chol in the trapped lipid domain of intact membranes. This new method complements the existing one, allowing acquisition of more detailed information about the distribution of lipids between domains in intact membranes. The methodological transition of the SR EPR spin-labeling approach from qualitative to quantitative is demonstrated. The abilities of this method are illustrated for intact cortical and nuclear fiber cell plasma membranes from porcine eye lenses. Statistical analysis (Student’s t test) of the data allowed determination of the separations of mean values above which differences can be treated as statistically significant (P ≤ 0.05) and can be attributed to sources other than preparation/technique. 相似文献
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63.
Baltisberger JH Musapelo T Sutton B Reynolds A Gurung L 《Journal of magnetic resonance (San Diego, Calif. : 1997)》2011,208(1):70-75
The primary shortcoming of the z-filtered refocused INADEQUATE MAS NMR pulse sequence is the possibility of artifacts introduced during the z-filter due to spin diffusion where by extra peaks in the single-quantum dimension (from other sites in the molecule) appear correlated with a given double-quantum frequency. This is a problem when the spinning speeds are too slow (less than 15 kHz) to sufficiently average the proton-proton homonuclear dipolar couplings. This would be especially important when working with large volume rotors that are difficult to spin fast enough to completely average the homonuclear couplings. In our experiments we used the frequency-switched Lee-Goldberg (FSLG) method of homonuclear decoupling during the z-filter to remove the artifact peaks. This method has the advantage of being quite easy to setup and implement on most modern NMR spectrometers. 相似文献
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Chavan Pramod V. Pandit Kapil S. Desai Uday V. Wadgaonkar Prakash P. Nawale Laxman Bhansali Sujit Sarkar Dhiman 《Research on Chemical Intermediates》2017,43(10):5675-5690
Research on Chemical Intermediates - A series of spirochromene-tethered 1,2,3-triazoles (1,2,3-triazolylspirochromenes) were designed and synthesized via click-chemistry-based one-pot... 相似文献
65.
Fe-doped TiO2, Ti1–xFexO2 (x = 0.00, 0.02, 0.04, 0.06, 0.08, and 0.10), photocatalysts have been successfully synthesized via citric acid–assisted autocombustion method. The synthesized photocatalysts were characterized using different characterization techniques, such as X-ray diffraction (XRD), diffuse reflectance spectroscopy (DRS), Fourier transform infrared (FT-IR), transmission electron microscopy (TEM), energy dispersive x-ray spectroscopy (EDX), and x-ray photoelectron spectroscopy (XPS). The XRD diffraction patterns revealed that synthesized photocatalysts have the anatase phase of TiO2. The DRS analysis indicates a slight increment in absorbance in the visible light region by the Fe doping in TiO2. The FT-IR spectra reveal the various stretching and bending vibrational bands of the Ti–O lattice. The XPS spectra confirm the presence of elements titanium, oxygen, and iron in the synthesized samples and determine binding energy of elements. TEM analysis shows the shape of the synthesized photocatalyst, and it was used to calculate the average particle sizes of undoped and Fe-doped TiO2 (Ti0.96Fe0.04O2) photocatalysts using a histogram. The photocatalytic activities of synthesized photocatalysts were determined by photodegradation of dye (Direct Blue 199), contaminating carpet industry wastewater in the photochemical reactor and open pan reactor. The maximum photodegradation activity was shown by the Ti0.96Fe0.04O2 photocatalyst among all the synthesized undoped and Fe-doped photocatalysts. The synthesized photocatalyst (Ti0.96Fe0.04O2) had better photocatalytic activity when compared to both, undoped TiO2 and Aeroxide (Degussa) P-25. The used Fe-doped TiO2 photocatalyst (Ti0.96Fe0.04O2) was regenerated five times and investigated for its photocatalytic activity. 相似文献
66.
Singh Laxman Kumar Atendra Lee Hansol Lee Jiwon Ji Minsoo Lee Youngil 《Journal of Solid State Electrochemistry》2018,22(8):2561-2568
Journal of Solid State Electrochemistry - This article reports for the first time ultrafast automatic flame synthesis of high-quality LiCoO2 in open-air conditions as a cathode material for Li-ion... 相似文献
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Potale Laxman V. Khodke Amol S. Patole Shangiresh S. Damle Mrinalini C. 《平面色谱法杂志一现代薄层色谱法》2012,25(1):72-76
JPC – Journal of Planar Chromatography – Modern TLC - Asensitive, selective, precise, and stability-indicating (in accordance with ICH guidelines) high-performance thin-layer... 相似文献
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JPC – Journal of Planar Chromatography – Modern TLC - Sida spinosa Linn. (synonyms: S. alba L., S. angustifolia Lam., S. angustifolia Mill.), Malvaceae, is an annual erect, branched... 相似文献
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JPC – Journal of Planar Chromatography – Modern TLC - A sensitive and accurate high-performance thin-layer chromatographic method has been developed, validated, and used for... 相似文献
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Aparna V. Deshpande Jayraj R. Rane Laxman V. Jathar 《Journal of Sol-Gel Science and Technology》2009,49(3):268-276
Glass samples are prepared with different amount of glycerol as drying control chemical additive (DCCA) via acid catalysed
sol–gel method. These samples are given solvent treatment namely rinse and dip with methanol while drying of the sample. In
rinse treatment solid sample is rinsed with small amount of methanol while in dip treatment sample is dipped for 5 h in methanol.
Comparative studies of these treated samples containing varied DCCA concentration are carried out by measuring optical transmission,
mechanical strength and bulk density. Various instrumental techniques used for analysis are FTIR, DTA-TGA, XRD, SEM and TEM.
The untreated samples take long time to dry up and to come out of the cuvette and show very less transmission in UV region
which is much enhanced by solvent treatment. On the basis of this study, the solvent treated glass samples with DCCA amount
8 ml in the composition used are found to have the maximum UV transmission, good mechanical strength and may be useful as
silica gel host matrices for solid state dye lasers and other applications. The UV transmission reported in the present studies
is 90% in 337 nm region, which is the wavelength of N2 laser pumping. 相似文献