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V. V. Gruzinskii V. S. Gorobchenko S. V. Davydov V. V. Eremenko I. I. Kulak A. I. Mit'kovets L. A. Ogurtsova 《Journal of Applied Spectroscopy》1990,53(3):984-987
Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 53, No. 3, pp. 461–464, September, 1990. 相似文献
37.
T Footz S Wunsam S Kulak H J Crabtree D M Glerum C J Backhouse 《Electrophoresis》2001,22(18):3868-3875
Sample preparation has long been recognized as a significant barrier to the implementation of macroscopic protocols on microfabricated devices. Macroscopically, such tasks as removing salts, primers and other contaminants are performed by methods involving precipitation, specialized membranes and centrifuges, none of which are readily performed in microfluidic structures. Although some microfluidic systems have been developed for performing sample purification, their complexity may hinder the degree to which they can be implemented. We present a method of microchip-based sample purification that can be performed with even the simplest microfluidic designs. The technique is demonstrated by removing primers from a sample of amplified DNA, leaving only the product DNA. This provides a new sample preparation capability for microfluidic systems. 相似文献
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G. V. Kulak 《Journal of Applied Spectroscopy》2001,68(4):649-655
Collinear acousto–optic interaction of light beams near the optical axes of biaxial crystals, including gyrotropic ones, is considered. Efficient acousto–optic transformation of fast and slow light beams under the conditions of internal conical refraction has been revealed. It is shown that the width of the transmission band and the central frequency of ultrasound in collinear acousto–optic filtration in a nongyrotropic crystal are determined by the angle at the apex of the cone of wave vectors (by the anisotropy of the crystal) and in a gyrotropic one by the projection of the gyration vector onto the direction of the optical axis. 相似文献
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G. V. Kulak 《Optics and Spectroscopy》2001,90(3):405-407
The collinear acoustooptical (AO) interaction of light beams under conditions of internal conical refraction is considered. The dependences of the diffraction efficiency on the ratio of radii of light and ultrasonic beams, the AO coupling length, and the ultrasound intensity are found. An analytical expression is obtained describing the diffraction efficiency of collinear AO conversion of annular beams of internal conical refraction. 相似文献
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I E Adzerikho A G Mrochek V V Dmitriev O A Lukyanchenko A I Kulak 《Ultrasonics sonochemistry》2001,8(3):315-318
Ultrasound fibrin clot destruction was investigated in vitro using electron microscopy and by monitoring the changes in the light transmission of clot debris suspension. It has been established that in the course of a combined action of ultrasound and fibrinolytic agent at high ultrasound intensities and short sonification periods, fibrin clot is disrupted mainly due to sonomechanical treatment, while fermentative lysis takes place in parallel and at a significantly lower rate. However, the streptokinase action prevails after ultrasound switching off and results in the prevention of clot debris conglomeration. 相似文献