A single-stranded human telomere DNA sequence can fold into an intramolecular G-quadruplex structure, which has been shown to inhibit telomerase activity. Small molecules that selectively target and stabilise the G-quadruplex structure have been proposed as potential anticancer drugs. In this study, we analysed the properties of binding of malachite green, a cationic triphenylmethane dye, to the G-quadruplex of d[(T2AG3)4] by UV spectroscopy of thermal melting analysis, a competitive equilibrium dialysis assay, and absorption and circular dichroism spectroscopies. When binding to malachite green, the quadruplex structure that formed in the presence of K+ ions was stabilised with an increase in melting temperatures by 6 °C. Malachite green showed selective binding to the G-quadruplex in the presence of duplex and single-stranded DNAs, owing to which it presents higher potential for anticancer therapy, compared to other triphenylmethane dyes. The induced signals of circular dichroism indicate that the binding mode of malachite green involves intercalation between adjacent guanine tetrads of the G-quadruplex. 相似文献
A new catalytic system for the dehydrogenative oxidation of alcohols using a water-soluble Cp*Ir complex bearing a bipyridine-based functional ligand as catalyst has been developed. With this catalytic system, a variety of primary and secondary alcohols have been efficiently converted to aldehydes and ketones, respectively, in aqueous media without using any oxidant. Reuse of the catalyst by a very simple procedure has been also accomplished. 相似文献
It is important that we understand the physical, chemical, and biological mechanisms that govern the interaction between nanoparticles (NPs) and heterogeneous cellular surfaces because of the possible cytotoxicity of engineered nanomaterials. In this study, we investigated the lateral localization of nano/microparticles within a biomimetic heterogeneous membrane interface using cell-sized two-phase liposomes. We found that lateral heterogeneity in the membrane mediates the partitioning of nano/microparticles in a size-dependent manner: small particles with a diameter of ≤200 nm were localized in an ordered phase, whereas large particles preferred a fluidic disordered phase. This partitioning behavior was verified by temperature-controlled membrane miscibility transition and laser-trapping of associated particles. In terms of the membrane elastic energy, we present a physical model that explains this localization preference of nano/microparticles. The calculated threshold diameter of particles that separates the particle-partitioning phase was 260 nm, which is in close agreement with our observation (200 nm). These findings may lead to a better understanding of the basic mechanisms that underlie the association of nanomaterials within a cell surface. 相似文献
Playing the sax : The enantioselective total syntheses of (?)‐ and (+)‐decarbamoyloxysaxitoxin (doSTX) and (+)‐saxitoxin (STX) are reported. A new methodology was developed for the synthesis of STXs, featuring discriminative reduction of the nitro group and N? O bond in nitroisoxazolidine.
Superconductivity in carbon nanotubes (CNTs) is attracting considerable attention. However, its correlation with carrier doping has not been reported. We report on the Meissner effect found in thin films consisting of assembled boron (B)-doped single-walled CNTs (B-SWNTs). We find that only B-SWNT films consisting of low boron concentration leads to evident Meissner effect with Tc=12 K and also that a highly homogeneous ensemble of the B-SWNTs is crucial. The first-principles electronic-structure study of the B-SWNTs strongly supports these results. 相似文献
Photoirradiation effect on potential response to metal ion concentrations and photoinduced potential change were investigated with poly(vinyl chloride) membranes based on a Malachite Green derivative carrying a bis(monoaza-15-crown-5) moiety, by comparing other Malachite Green derivatives. The Malachite Green carrying a bis(crown ether) moiety caused a potential response to potassium ion concentration changes under dark condition. In the membrane potential response, a clear-cut photoinduced switching of potential response was realized by the membrane of Malachite Green carrying a bis(crown ether) moiety, which exhibited no potential response to potassium ion concentrations (0 mV/decade) on UV irradiation. On the other hand, a Malachite Green carrying a monocyclic benzocrown ether moiety showed a considerable dependence of the membrane potential on the metal ion concentrations under both dark and UV irradiation conditions. 相似文献
Copoly(γ-stearyl L-glutamate-γ-methyl L-glutamate)s with various compositions were synthesized by ester exchange of poly(γ-methyl L-glutamate) with stearyl alcohol. The temperature dependence of the volume and helical spacing of the copolyglutamates thus prepared was examined from 0 to 100°C, observing the melting and crystallization of the long alkyl side chain attached to the polypeptide backbone. The melting temperature of the long side chain increased with increasing stearyl L-glutamate content. With increasing temperature, the α helical spacing decreased, especially with the melting of the side chain. On the other hand, the volume increased with increasing temperature and a marked increase was observed on melting. The above two phenomena were elucidated according to the structural model of the long side chain of the copolyglutamates proposed here. The transport properties, permeability coefficients were found to be considerably affected by the side-chain structure of the copolyglutamate and by the melting or crystallization of the side chain. The permeability coefficient of the copolyglutamate with 67% degree of stearylation, varied as much as two orders of magnitude before and after melting or crystallization. It was also confirmed that the permeability coefficient is controllable by the side-chain structural change of polypeptides. 相似文献