Disposable sodium electrodes

In this paper we describe a disposable sodium sensor in double matrix membrane technology. This sensor is prepared from filter paper with an evaporated silver conducting line on one side. For insulation the sensor is laminated with a pre-perforated heat-sealing film. A defined volume of an ion-sensitive polymer matrix membrane cocktail is filled into one hole. So an ion-sensitive coated film sensor in double matrix membrane technology is produced. The double matrix membrane is formed by the polymer matrix membrane and by the additional filter paper matrix. The response behaviour of the sodium electrode is comparable with conventional macro ion-selective electrodes. By this technology a mass production of low cost sensors is possible.     

Pseudohalogenometallverbindungen: LVIII. Reaktionen von diphenylphosphorylazid und perfluor-n-hexylsulfonylazid mit triphenylphosphan-komplexen von platin(0), rhodium(I), iridium(I), ruthenium(0), kobalt(II) und kupfer(I)

Diphenylphosphorylazide N₃P(O)(OPh)₂ reacts with Pt(PPh₃)₃, Pt(PPh₃)₂(C₂H₄), trans-RhCl(CO)(PPh₃)₂, Ru(CO)₃(PPh₃)₂, CoCl₂(PPh₃)₂ and CuCl(PPh₃)₂ to give the azido complexes Pt(PPh₃)₂(N₃)R, Pt(PPh₃)₂(N₃)₂R₂, the urylene complex RhCl(PPh₃)₂(RNCONR) and the phosphine imine complexes Ru(CO)₃(RPPh₃)₂, CoCl₂(RNPPh₃)₂, CuCl(RNPPh₃)₂, respectively, (RP(O)(OPh)₂). The oxidative addition of n-C₆F₁₃SO₂N₃ to Pt(PPh₃)₄ and Pt(PPh₃)₂(C₂H₄) affords the complexes Pt(PPh₃)₂(N₃)R and Pt(PPh₃)₂(N₃)₂R₂, respectively, (RSO₂C₆F₁₃. The compounds are characterized by elemental analysis and by their IR spectra.     

A single-fluor approach to DNA sequence determination using high performance capillary electrophoresis.

The Tabor and Richardson strategy for enzymatic chain termination sequencing of DNA using relative peak intensity has been adapted to high performance capillary gel electrophoresis with laser induced fluorescence detection. This approach to DNA sequencing involves the use of only a single fluor and results in significant reduction in the time required to determine a DNA sequence without the use of highly complicated and expensive instrumentation. We present a modification of the Tabor and Richardson approach employing two reactions, each containing complementary mixtures of only three ddNTP's in the concentration ratio 4:2:1. The DNA sequence is determined by relative peak height and by assigning the missing ddNTP to "gaps" between the peaks. The use of only three terminators/reaction simplifies the software task of differentiating between the termination types and makes more efficient use of the available dynamic range. Both complementary mixes generate complete sequence information and the two data files are combined in order to make a more confident sequence call. This process helps to eliminate errors caused by occasional non-uniform incorporation of ddNTP's or false terminations and also alleviates some of the difficulty associated with reading through compressed regions of the electropherogram.     

Sample evaporation in conventional GC split/splitless injectors. Part 1: Some quantitative estimates concerning heat consumption during evaporation

During sample evaporation in conventional vaporizing injection, the supply of heat to the evaporating liquid is a problem, first because the amounts of heat consumed are relatively large and, secondly, because the heat must be transferred to the sample within a very short time. Times available for evaporation, required amounts of heat, possible sources of heat, and the time required to transfer the heat to the sample liquid are discussed. It is shown that mixing with carrier gas contributes little heat to the evaporation process, but also that packings with glass wool have too low a heat capacity to deliver the required amount of heat to the evaporating sample. Transfer of heat from the insert wall to the sample easily requires several seconds, even if cooling of the vaporizing zone by 20° is accepted. Thus “flash evaporation” is usually impossible and most liquids must be held in the vaporizing chamber to allow full evaporation.     

Sample evaporation in conventional split/splitless GC injectors. Part 3: Retaining the liquid in the vaporizing chamber

As most sample liquids tend to pass through an empty injector insert at a speed which is too high to enable complete evaporation, movement of the liquid must be arrested before it reaches the column entrance. Stopping the liquid means deposition on to a surface; this, however, is possible only after the temperature of the surface has been cooled to (or below) the boiling point of the liquid (solvent). The performance of different means of stopping the liquid has been tested visually (by the method described in Part 2). Baffles on the wall of the injector insert had hardly any effect on evaporation: the band of liquid leaving the syringe needle performed a perfect slalorn around them. The inverted cup proved more efficient, but the best performance was obtained from a light plug of glass wool: owing to its low thermal mass, the first fibers to be met by the liquid are immediately cooled to the solvent boiling point, allowing the liquid to wet it. The sample liquid is sucked up by the glass wool, from where the sample evaporates relatively slowly, often over a period of several seconds.     

Scaling function for the energy density correlation at large momentum

The static energy-density correlation function S ²(0)S ²(x)–S ²² is calculated in the critical region for theS ⁴ Ginzburg-Landau-Wilson model at short distances and for nonzero field. Short distance expansion is used and its structure for more complex vertex functions is given. Goldstone mode singularities present at the magnetization curve are taken into account. The main application is given in the theory of polymer solutions. Here, S ²(0)S ²(x)–S ²² becomes the Fourier transform of the densitydensity correlation.     

OXYGEN QUENCHING OF CHLOROPHYLL FLUORESCENCE IN CHLOROPLASTS

Abstract— Three phases of chlorophyll a fluorescence quenching by O₂ are observed in green plants. The effects of various inhibitors on photosynthetic partial processes in chloroplasts were investigated in attempts to (1) localize the O₂-quenching sites and (2) assess possible physiological significance of O₂-quenching. Our results localize the most sensitive (and presumably functionally important) phase to a site between plastoquinone and the photosystem I acceptor, chlorophyll (P₇₀₀), possibly plastocyanin. It is suggested that PC may transfer electrons to oxygen in addition to P₇₀₀.     

DBU-assisted 1,3,2-oxathiaphospholane ring-opening condensation with selected O-, S-, N- and C-nucleophiles

The reactivity of protected thymidine 3′-O- and 5′-O-(2-thio-1,3,2-oxathiaphospholanes) towards various nucleophiles in the presence of DBU is presented and mechanistic implications are discussed.     

Azobenzene-containing polyamic acid with excellent Langmuir-Blodgett-Kuhn film formation behavior suitable for all-optical switching

To generate command surfaces for all-optical switching, highly ordered polymeric Langmuir-Blodgett-Kuhn (LBK) multilayers were fabricated onto silicon substrates and gold-coated optical glass slides from novel azobenzene-bearing polyamic acid systems. Pronounced Bragg peaks and well-defined Kiessig fringes observed in the X-ray reflectivity measurement for samples on silicon substrates indicate that these films possess a regularly repeated Y-type LBK multilayer structure and ultrasmooth surfaces. Fourier transform infrared (FT-IR) spectra taken by grazing incidence reflection suggest specific orientations of the functional groups in the layers. The excellent film-forming properties of the polyamic acid allow for a smooth buildup of several hundreds of layers of the LBK films onto gold-coated glass slides, which in turn allows for determining the geometrical thickness and the anisotropic refractive indices of the films by using optical waveguide spectroscopy. Interestingly, the probe laser beam induced a distinct fluorescence signal from the films, which remained even after the film underwent a thermal imidization process at 160 degrees C for 8 h in vacuo. LBK films fabricated from these compounds can be successfully applied for all-optically switching the alignment of liquid crystals by irradiation with light of different wavelengths.