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AUTOFLUORESCENCE SPECTROSCOPY OF OPTICALLY TRAPPED CELLS 总被引:2,自引:0,他引:2
Karsten Konig Yagang Liu Greg J. Sonek Michael W. Berns Bruce J. Tromberg 《Photochemistry and photobiology》1995,62(5):830-835
Abstract— Cellular autofluorescence spectra were monitored in a single-beam gradient force optical trap ("optical tweezers") in order to probe the physiological effects of near infrared and UVA (320–400 nm) microirradiation. Prior to trapping, Chinese hamster ovary cells exhibited weak UVA-excited autofluorescence with maxima at 455 nm characteristic of β-nicotinamide adenine dinucleotide (phosphate) emission. No strong effect of a 1064 nm NIR microbeam on fluorescence intensity and spectral characteristics was found during trapping, even for power densities up to 70 MW/cm2 and radiant exposures of 100 GJ/cm2 . In contrast to the 1064 nm trap, a 760 nm trapping beam caused a two-fold autofluorescence increase within 5 min (about 20 GJ/cm2 ). Exposure to 365 nm UVA (1 W/cm2 ) during 1064 nm trapping significantly altered cellular autofluorescence, causing, within 10 min, a five-fold increase and a 6 nm red shift versus initial levels. We conclude that 1064 nm microbeams can be applied for an extended period without producing autofluorescence changes characteristic of alterations in the cellular redox state. However, 760 nm effects may occur via a two-photon absorption mechanism, which, in a manner similar to UVA exposure, alters the redox balance and places the cell in a state of oxidative stress. 相似文献
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G C Paddon-Jones C S McErlean P Hayes C J Moore W A Konig W Kitching 《The Journal of organic chemistry》2001,66(22):7487-7495
A palladium(II)-catalyzed hydroxycyclization-carbonylation-lactonization sequence with appropriate pent-4-ene-1,3-diols provides efficient access to the bicyclic gamma-lactones, 5-n-butyl- and 5-n-hexyltetrahydrofuro-[3,2-b]furan-2(3H)-ones (3) and (4), respectively, in both racemic and enantiomeric forms. Some of the substrate pent-4-ene-1,3-diols of high enantiomeric excess (ee) have been derived from racemic terminal epoxides by hydrolytic kinetic resolution (HKR) using cobalt (III)-salen complexes. (9Z,12R)-(+)-Ricinoleic acid also serves as a "chiral pool" source of other pent-4-ene-1,3-diols. These syntheses and enantioselective gas chromatography confirm the structures and absolute stereochemistry of the lactones in some species of parasitic wasps (Hymenoptera: Braconidae). The highly abundant 5-n-hexyltetrahydrofuro-[3,2-b]furan-2(3H)-one (4) in Diachasmimorpha kraussii and D. longicaudata is of high ee (>99%) with (3aR,5R,6aR) stereochemistry. 相似文献
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Theory of diluted magnetic semiconductor ferromagnetism 总被引:1,自引:0,他引:1
We present a theory of carrier-induced ferromagnetism in diluted magnetic semiconductors ( III1-xMnxV) which allows for arbitrary itinerant-carrier spin polarization and dynamic correlations. Both ingredients are essential in identifying the system's elementary excitations and describing their properties. We find a branch of collective modes, in addition to the spin waves and Stoner continuum which occur in metallic ferromagnets, and predict that the low-temperature spin stiffness is independent of the strength of the exchange coupling between magnetic ions and itinerant carriers. We discuss the temperature dependence of the magnetization and the heat capacity. 相似文献
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Let F be a meromorphic function in the complex plane. We investigatethe behaviour of the iterates of F in a Baker domain B. In particular,we describe the dynamics of the orbits with the help of conformalconjugacies; that is, we determine a function which is univalentin a large simply connected subdomain of B such that (F(z))=T((z))holds throughout B. Here T is either a parabolic or hyperbolicMöbius transformation mapping either a half plane or Conto itself. This functional equation is always solvable ina Baker domain if F has only finitely many poles. Moreover,there is an example of a function with infinitely many poleswhere one cannot find an appropriate conformal conjugacy inan invariant Baker domain. 相似文献
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Martinvaldo Konig 《International Journal of Theoretical Physics》2014,53(10):3409-3422
Heyting effect algebras are lattice-ordered pseudoboolean effect algebras endowed with a pseudocomplementation that maps on the center (i.e. Boolean elements). They are the algebraic counterpart of an extension of both ?ukasiewicz many-valued logic and intuitionistic logic. We show that Heyting effect algebras are termwise equivalent to Heyting-Wajsberg algebras where the two different logical implications are defined as primitive operators. We prove this logic to be decidable, to be strongly complete and to have the deduction-detachment theorem. 相似文献