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101.
Ghulam Murtza Anjum Weidong Du Rachel Klein Umme Amara Markus Huber‐Lang E. Marion Schneider Peter Wiegand 《Electrophoresis》2010,31(2):309-314
Forensic analysis of mitochondrial displacement loop (D‐loop) sequences using Sanger sequencing or SNP detection by minisequencing is well established. Pyrosequencing has become an important alternative because it enables high‐throughput analysis and the quantification of individual mitochondrial DNAs (mtDNAs) in samples originating from more than one individual. DNA typing of the mitochondrial D‐loop region is usually the method of choice if STR analysis fails because of trace amounts of DNA and/or extensive degradation. The main aim of the present work was to optimize the efficiency of pyrosequencing. To do this, 31 SNPs within the hypervariable regions I and II of the D‐loop of human mtDNA were simultaneously analyzed. As a novel approach, we applied two sets of amplification primers for the multiplexing assay. These went in combination with four sequencing primers for pyrosequencing. This method was compared with conventional sequencing of mtDNA from blood and biological trace materials. 相似文献
102.
Naser A. Anjum Sarvajeet S. Gill Armando C. Duarte Eduarda Pereira Iqbal Ahmad 《Journal of nanoparticle research》2013,15(9):1-26
Silver nanoparticles (AgNPs) have broad spectrum antimicrobial/biocidal properties against all classes of microorganisms and possess numerous distinctive physico-chemical properties compared to bulk Ag. Hence, AgNPs are among the most widely used engineered NPs in a wide range of consumer products and are expected to enter natural ecosystems including soil via diverse pathways. However, despite: (i) soil has been considered as a critical pathway for NPs environmental fate, (ii) plants (essential base component of all ecosystems) have been strongly recommended to be included for the development of a comprehensive toxicity profile for rapidly mounting NPs in varied environmental compartments, and (iii) the occurrence of an intricate relationship between “soil–plant systems” where any change in soil chemical/biological properties is bound to have impact on plant system, the knowledge about AgNPs in soils and investigations on AgNPs–plants interaction is still rare and in its rudimentary stage. To this end, the current paper: (a) overviews sources, status, fate, and chemistry of AgNPs in soils, AgNPs-impact on soil biota, (b) critically discusses terrestrial plant responses to AgNPs exposure, and (c) illustrates the knowledge-gaps in the current perspective. Based on the available literature critically appraised herein, a multidisciplinary integrated approach is strongly recommended for future research in the current direction aimed at unveiling the rapidly mounting AgNPs-fate, transformation, accumulation, and toxicity potential in “soil–plant systems,” and their cumulative impact on environmental and human health. 相似文献
103.
A simple and cost effective long reach spectrum sliced WDM-PON architecture for 32 users is proposed in which multiple PONs are integrated which further increase utilization of bandwidth and network sharing between the users. It is a low cost network which uses LEDs as a broadband light emitting source and further increases equipment sharing between users. The feasibility of the architecture is analyzed using BER analysis. The architecture survives up to 150 + 20 km fiber length where BER values of each received channel are ≤10−9. 相似文献
104.
Phytochemicals such as polyphenols and carotenoids are gaining importance because of their contribution to human health and
their multiple biological effects such as antioxidant, antimutagenic, anticarcinogenic, and cytoprotective activities and
their therapeutic properties. Banana peel is a major by-product in pulp industry and it contains various bioactive compounds
like polyphenols, carotenoids, and others. In the present study, effect of ripening, solvent polarity on the content of bioactive
compounds of crude banana peel and the protective effect of peel extracts of unripe, ripe, and leaky ripe banana fruit on
hydrogen peroxide-induced hemolysis and their antioxidant capacity were investigated. Banana (Musa paradisica) peel at different stages of ripening (unripe, ripe, leaky ripe) were treated with 70% acetone, which were partitioned in
order of polarity with water, ethyl acetate, chloroform (CHCl3), and hexane sequentially. The antioxidant activity of the samples was evaluated by the red cell hemolysis assay, free radical
scavenging (1,1-diphenyl-2-picrylhydrazyl free radical elimination) and superoxide dismutase activities. The Folin–Ciocalteu's
reagent assay was used to estimate the phenolic content of extracts. The findings of this investigation suggest that the unripe
banana peel sample had higher antioxidant potency than ripe and leaky ripe. Further on fractionation, ethyl acetate and water
soluble fractions of unripe peel displayed high antioxidant activity than CHCl3 and hexane fraction, respectively. A positive correlation between free radical scavenging capacity and the content of phenolic
compound were found in unripe, ripe, and leaky ripe stages of banana peel. 相似文献
105.
Sarot Cheenpracha Chatchanok Karalai Chanita Ponglimanont Kan Chantrapromma Shazia Anjum 《Tetrahedron》2005,61(36):8656-8662
Nine new cassane-type diterpenes, named taepeenin A-I, and two new norcassane-type diterpenes, named nortaepeenin A-B, were isolated from the stems and roots of Caesalpinia crista along with three known diterpenes: vinhaticoic acid, methyl vinhaticoate and ent-11β-hydroxy-rosa-5,15-diene. Their structures were elucidated on the basis of spectroscopic analysis. In addition, the structure of taepeenin A was confirmed by X-ray diffraction analysis. 相似文献
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108.
Hari M. Srivastava Komal Khatterwani S. K. Upadhyay 《Mathematical Methods in the Applied Sciences》2019,42(9):3103-3122
In the present paper, a fractional wavelet transform of real order α is introduced, and various useful properties and results are derived for it. These include (for example) Perseval's formula and inversion formula for the fractional wavelet transform. Multiresolution analysis and orthonormal fractional wavelets associated with the fractional wavelet transform are studied systematically. Fractional Fourier transforms of the Mexican hat wavelet for different values of the order α are compared with the classical Fourier transform graphically, and various remarkable observations are presented. A comparative study of the various results, which we have presented in this paper, is also represented graphically. 相似文献
109.
P. S. Ramanjaneyulu Komal Chandra A. S. Kulkarni C. S. Yadav Abhijit Saha M. K. Saxena B. S. Tomar K. L. Ramakumar 《Journal of Radioanalytical and Nuclear Chemistry》2014,301(1):117-124
Total gas content and its composition are important specifications for sintered nuclear fuel pellets particularly in the case of fast breeder reactor fuels. Most commonly, total gas content and its composition is determined by hot vacuum extraction-quadrupole mass spectrometry (HVE-QMS). A number of parameters in this methodology such as temperature, duration of heating for quantitative extraction of evolved gases, total volume of the system, gas analysis conditions etc. need to be optimized for reliable measurements. In addition, sensitivity factors for various gases like H2, CH4, N2, CO, O2 and CO2 in quadrupole mass spectrometry required for quantification of results have been determined and validated employing reference gas mixtures of known composition. Employing these optimized conditions total gas content and its composition in blanket pellets (uranium oxide pellets) of Indian prototype fast breeder reactor was determined employing HVE-QMS. The relative expanded uncertainty (at a coverage factor k = 2) in the measurement of total gas content excluding hydrogen was estimated as per ISO guidelines and it was found to be 9.2 %. 相似文献
110.