Fructosamine-6-kinases (FN6Ks) that catalyze phosphorylation of glycated amino acids, i.e., fructosyl amino acids (FAs), have been shown as a potential recognition element for glycated protein detection. However, there are only two available FN6Ks: those from Escherichia coli which is specific for ε-fructosyl lysine (ε-FK) and Bacillus subtilis which recognizes both ε-FK and α-FA as substrates. In this study, we characterized an FN6K homologue isolated from Arthrobacter, some of whose species are reported to assimilate FA. The BLAST searches of Arthrobacter genomic database, using the bacterial FN6K primary structure information, revealed the presence of an FN6K homologue in Arthrobacter aurescens TC1 strain. Indeed, enzymatic assays confirmed that the putative FN6K from A. aurescens is an FN6K that is specific for ε-FK, although the primary sequence alignments showed similarity of A. aurescens FN6Ks with FN6Ks from B. subtilis and E. coli at the same level. In this study, we describe for the first time the presence of FN6K in Arthrobacter spp. and ε-FK-specific degradation pathway from Gram-positive bacteria, providing important information for the development of FA-recognizing molecules as well as for the FA assimilation system in bacteria. 相似文献
We have developed a Cd2+ fluorescent chemosensor with high selectivity as well as sensitivity by tethering a 4,7-bis(2-hydroxyethyl)-9-hydroxy-1,4,7-triazanonyl chelator to anthracene. This sensor features the ability to discriminate Cd2+ from Zn2+ to a high degree (KdZn/KdCd = 560) in a pH 7.2 buffer. 相似文献
In this study, we have demonstrated a two-legged, upright molecular design method for monochromatic and bright red luminescent LnIII-silica nanomaterials. A novel EuIII-silica hybrid nanoparticle was developed by using a doubly binding TPPO−Si(OEt)3 (TPPO: triphenyl phosphine oxide) linker. The TPPO−Si(OEt)3 was confirmed by 1H, 31P, 29Si NMR spectroscopy and single-crystal X-ray analysis. Luminescent Eu(hfa)3 and Eu(tfc)3 moieties (hfa: hexafluoroacetylacetonate, tfc: 3-(trifluoromethylhydroxymethylene)camphorate) were fixed onto TPPO−Si(OEt)3-modified silica nanoparticles, producing Eu(hfa)3(TPPO−Si)2-SiO2 and Eu(tfc)3(TPPO−Si)2-SiO2, respectively. Eu(hfa)3(TPPO−Si)2−SiO2 exhibited the higher intrinsic luminescence quantum yield (93 %) and longer emission lifetime (0.98 ms), which is much larger than those of previously reported EuIII-based hybrid materials. Eu(tfc)3(TPPO−Si)2−SiO2 showed an extra-large intrinsic emission quantum yield (54 %), although the emission quantum yield for the precursor Eu(tfc)3(TPPO−Si(OEt)3)2 was found to be 39 %. These results confirmed that the TPPO−Si(OEt)3 linker is a promising candidate for development of EuIII-based luminescent materials. 相似文献
A NiCl2(PEt3)2-catalyzed regioselective C−H coupling of 8-aminoquinoline-derived benzamides with oxetanes has been developed. The reaction proceeds with concomitant removal of the 8-aminoquinoline auxiliary to directly form the corresponding seven-membered benzolactones, which frequently occur in natural products and bioactive molecules. Additionally, no stereochemical erosion is observed during the course of the reaction, and the use of enantioenriched and substituted oxetane thus provides a new avenue to the optically active benzolactone. 相似文献
Oxygen-sensitive and near-infrared (NIR) luminescent YbIII coordination polymers incorporating ligands based on pyrene derivatives were synthesized: YbIII–TBAPy and YbIII–TIAPy (TBAPy: 1,3,6,8-tetrakis(p-benzoate)pyrene; TIAPy: 1,3,6,8-tetrakis(3,5-isophthalic acid)pyrene). The coordination structures of these materials have been characterized by means of electrospray ionization mass spectrometry, X-ray diffraction analysis, and thermogravimetric analysis. Moreover, the porous structure of YbIII–TIAPy has been evaluated by measuring its N2 adsorption isotherm. The NIR luminescence properties of YbIII–TBAPy and YbIII–TIAPy have been examined by acquiring emission spectra and determining emission lifetimes under air or argon and in vacuo. YbIII–TIAPy exhibited high thermal stability (with a decomposition temperature of 400 °C), intense luminescence (with an emission quantum yield under argon of 6.6 %), and effective oxygen-sensing characteristics. These results suggest that NIR luminescent YbIII coordination polymers prepared using pyrene derivatives could have applications in novel thermo-stable oxygen sensors. 相似文献
We investigate a hidden-variable theory introduced by Kochen and Specker. The “hidden” results of measurements are either 1 or − 1. We suppose the validity of Sum rule and Product rule. Kochen and Specker suppose the two operations Sum rule and Product rule commute with each other. It is shown that the two operations Sum rule and Product rule do not commute with each other when we want to avoid the Kochen and Specker paradox. Otherwise we encounter the Kochen and Specker paradox. We mention the supposition for Greenberger, Horne, and Zeilinger paradox. It is discussed that only Product rule is necessary for the paradox. We give up the two paradoxes if (1) Sum rule and Product rule do not commute with each other and (2) Product rule is not valid.