Luminol-dependent chemiluminescence analysis of cellular and humoral defects of phagocytosis using a chem-glo photometer

In summary, this report describes the conditions under which luminol has been utilized to measure phagocytosis-associated metabolic events in activated human PMNs and rabbit and dog alveolar macrophages. We feel that this system may have wide applicability to both clinical and experimental situations. Some possible applications are shown in the following table.

Potential Applications of Luminol-Amplified Chemiluminescence

     

73.

Allowance for polydispersity in the determination of the true plate height in GPC     

J. H. Knox  F. McLennan 《Chromatographia》1977,10(2):75-78

     

74.

Glutamic acid 181 is negatively charged in the bathorhodopsin photointermediate of visual rhodopsin     

Sandberg MN  Amora TL  Ramos LS  Chen MH  Knox BE  Birge RR 《Journal of the American Chemical Society》2011,133(9):2808-2811

Assignment of the protonation state of the residue Glu-181 is important to our understanding of the primary event, activation processes and wavelength selection in rhodopsin. Despite extensive study, there is no general agreement on the protonation state of this residue in the literature. Electronic assignment is complicated by the location of Glu-181 near the nodal point in the electrostatic charge shift that accompanies excitation of the chromophore into the low-lying, strongly allowed ππ* state. Thus, the charge on this residue is effectively hidden from electronic spectroscopy. This situation is resolved in bathorhodopsin, because photoisomerization of the chromophore places Glu-181 well within the region of negative charge shift following excitation. We demonstrate that Glu-181 is negatively charged in bathorhodopsin on the basis of the shift in the batho absorption maxima at 10 K [λ(max) band (native) = 544 ± 2 nm, λ(max) band (E181Q) = 556 ± 3 nm] and the decrease in the λ(max) band oscillator strength (0.069 ± 0.004) of E181Q relative to that of the native protein. Because the primary event in rhodopsin does not include a proton translocation or disruption of the hydrogen-bonding network within the binding pocket, we may conclude that the Glu-181 residue in rhodopsin is also charged.     

75.

Electrochromatography in packed tubes using 1.5 to 50 μm silica gels and ODS bonded silica gels     

J. H. Knox  I. H. Grant 《Chromatographia》1991,32(7-8):317-328

Summary Electrochromatography (that is HPLC where the eluent is driven along the column by electro-osmosis using fields of up to 100 kV m⁻¹) promises plate efficiencies for HPLC which are comparable to those attained in capillary gas chromatography, but this requires that narrow-bore columns can be successfully packed with submicron particles. This paper demonstrates that we have now moved a considerable distance towards this goal. We show (1) that, following theory, there is no evidence of any reduction in electroosmotic velocity in columns packed with particles down to 1.5 μm diameter, (2) that reduced plate heights as low as unity are attainable for unretained solutes using both slurrypacked and drawn-packed columns 30 to 200 μm bore and up to 1 m long when packed with conventional 3 and 5 μm silica gels or with 1.5 μm impermeable silica spheres, (3) that columns driven electrically show higher plate efficiencies than identical columns driven by pressure, and (4) that 100,000 plate HPLC separations can be achieved in relatively short times of 30 minutes using in situ derivatised drawn packed capillaries containing 3 and 5 μm ODS-silica gels.     

76.

Experience sampling: Assessing urban soundscapes using in-situ participatory methods     

Adam Craig  David MooreDon Knox 《Applied Acoustics》2017

     

77.

Book reviews     

E. R. Adlard  J. E. Futter  K. Jones  J. H. Knox 《Chromatographia》1994,39(11-12):747-749

     

78.

Differential cross section and polarization for 2.63 MeV neutrons scattered from 12C     

H.D. Knox  J.M. Cox  R.W. Finlay  R.O. Lane 《Nuclear Physics A》1973,217(3):611-627

The differential cross section and polarization of 2.63 MeV neutrons scattered from ¹²C have been measured at eight angles between 17° and 118° in the laboratory system. By simultaneously fitting the cross section and polarization data, a set of scattering phase shifts was obtained. The values of the resulting d-wave phase shifts were larger than those of other existing sets of phase shifts in the energy region. A subsequent R-function analysis, reflecting these larger d-wave phase shifts, gave excellent fits to other experimental data below 3 MeV neutron energy region. The influence of narrow states at 7.50 and 7.55 MeV excitation energy in ¹³C is discussed.     

79.

Picosecond time delay fluorimetry using a jitter-free streak camera     

Michael Stavola  Gerard Mourou  Wayne Knox 《Optics Communications》1980,34(3):404-408

A streak camera driven with picosecond accuracy by a laser-activated cryogenic Si-switch has been used to measure the decay time of malachite green in water by a pulse fluorimetry technique. The single shot timing accuracy of the streak camera (a few ps) corresponds to ～10% of the optical pulsewidth. The overall stability of the system allows the averaging of hundreds of laser shots without image translation improving both the signal to noise ratio and timing accuracy of the measurements.. The subpicosecond timing accuracy afforded by shot averaging has enabled us to measure a 2 ps fluorescence decay time for a low quantum yield sample with a 20 ps excitation pulse.     

80.

Wechselwirkung zwischen Aerosol O T und Gelatine     

W. J. Knox Jr  T. O. Parschall 《Colloid and polymer science》1974,252(7-8):623-623

     

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Cell type	Applications
Human polymorphonuclear leukocytes	(1) Detection of bactericidal defects, particularly chronic granulomatous disease
	(2) Detection of host opsonic defects (both immunoglobulin and complement ?C3b opsonic defects).
	(3) Analysis of drug effects on host cellular and opsonic defenses (9,11).
	(4) Characterization of bacteria or other particulate matter in terms of ability to generate opsonic activity and/or be ingested by phagocytic cells (3,7).
Alveolar macrophages	(1) Detection of environmental pollutant effects on respiratory defense mechanisms (against both particulate and soluble matter).
	(2) Analysis of drug effects on respiratory defense mechanisms, particularly drugs administered in the treatment of respiratory diseases.

Glutamic acid 181 is negatively charged in the bathorhodopsin photointermediate of visual rhodopsin

Assignment of the protonation state of the residue Glu-181 is important to our understanding of the primary event, activation processes and wavelength selection in rhodopsin. Despite extensive study, there is no general agreement on the protonation state of this residue in the literature. Electronic assignment is complicated by the location of Glu-181 near the nodal point in the electrostatic charge shift that accompanies excitation of the chromophore into the low-lying, strongly allowed ππ* state. Thus, the charge on this residue is effectively hidden from electronic spectroscopy. This situation is resolved in bathorhodopsin, because photoisomerization of the chromophore places Glu-181 well within the region of negative charge shift following excitation. We demonstrate that Glu-181 is negatively charged in bathorhodopsin on the basis of the shift in the batho absorption maxima at 10 K [λ(max) band (native) = 544 ± 2 nm, λ(max) band (E181Q) = 556 ± 3 nm] and the decrease in the λ(max) band oscillator strength (0.069 ± 0.004) of E181Q relative to that of the native protein. Because the primary event in rhodopsin does not include a proton translocation or disruption of the hydrogen-bonding network within the binding pocket, we may conclude that the Glu-181 residue in rhodopsin is also charged.     

Electrochromatography in packed tubes using 1.5 to 50 μm silica gels and ODS bonded silica gels

Summary Electrochromatography (that is HPLC where the eluent is driven along the column by electro-osmosis using fields of up to 100 kV m⁻¹) promises plate efficiencies for HPLC which are comparable to those attained in capillary gas chromatography, but this requires that narrow-bore columns can be successfully packed with submicron particles. This paper demonstrates that we have now moved a considerable distance towards this goal. We show (1) that, following theory, there is no evidence of any reduction in electroosmotic velocity in columns packed with particles down to 1.5 μm diameter, (2) that reduced plate heights as low as unity are attainable for unretained solutes using both slurrypacked and drawn-packed columns 30 to 200 μm bore and up to 1 m long when packed with conventional 3 and 5 μm silica gels or with 1.5 μm impermeable silica spheres, (3) that columns driven electrically show higher plate efficiencies than identical columns driven by pressure, and (4) that 100,000 plate HPLC separations can be achieved in relatively short times of 30 minutes using in situ derivatised drawn packed capillaries containing 3 and 5 μm ODS-silica gels.     

Differential cross section and polarization for 2.63 MeV neutrons scattered from 12C

The differential cross section and polarization of 2.63 MeV neutrons scattered from ¹²C have been measured at eight angles between 17° and 118° in the laboratory system. By simultaneously fitting the cross section and polarization data, a set of scattering phase shifts was obtained. The values of the resulting d-wave phase shifts were larger than those of other existing sets of phase shifts in the energy region. A subsequent R-function analysis, reflecting these larger d-wave phase shifts, gave excellent fits to other experimental data below 3 MeV neutron energy region. The influence of narrow states at 7.50 and 7.55 MeV excitation energy in ¹³C is discussed.     

Picosecond time delay fluorimetry using a jitter-free streak camera

A streak camera driven with picosecond accuracy by a laser-activated cryogenic Si-switch has been used to measure the decay time of malachite green in water by a pulse fluorimetry technique. The single shot timing accuracy of the streak camera (a few ps) corresponds to ～10% of the optical pulsewidth. The overall stability of the system allows the averaging of hundreds of laser shots without image translation improving both the signal to noise ratio and timing accuracy of the measurements.. The subpicosecond timing accuracy afforded by shot averaging has enabled us to measure a 2 ps fluorescence decay time for a low quantum yield sample with a 20 ps excitation pulse.