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141.
A simple semiconductor gas sensor (TGS 812) is used for the on-line measurement and control of indole during the production of l-tryptophan from indole and l-serine with immobilized E. coli cells. Indole is estimated in the reactor gas space. In combination with an automatic indole supply system, a feed-batch process became possible. The indole concentration was monitored and kept within the optimal range (300–600 mg l?1). A simple gas-sensing electrode dipped in the reaction medium provides direct measurement of organic solvents and gases in the liquid. Such a system is suitable for on-line determination of ethanol (10–70 g l?1) during continuous production of ethanol with immobilized yeast cells. 相似文献
142.
A method is described for the NMR determination of the enantiomeric composition of chiral carboxylic acids and alcohols using their triphenyltin derivatives together with the chiral solvating agents quinine hydrochloride and phenylethylamine. 相似文献
143.
Improved STR typing of telogen hair root and hair shaft DNA 总被引:1,自引:0,他引:1
Today the STR typing of telogen hair and hair shafts is regarded as a challenge. The small DNA quantity in the hair is highly degraded. Another problem are PCR inhibitors in the hair. In particular hair pigments, the melanins, are known to inhibit PCR. Hairs are exposed to sunlight and partly to chemical oxidation processes, which make them even more difficult to analyze. To increase the chances of a correct typing of hair, the small amount of DNA must be successfully isolated and the inhibitors have to be removed or neutralized. Furthermore, miniSTR typing improves the analysis of stains with degraded DNA like it is the case with hair. We introduce a nonorganic extraction method and in addition a miniSTR concept which is promising in typing stains with little and degraded DNA, especially hairs. The miniSTR concept including five database STRs (SE33, VWA, TH01, FGA, D3S1358) and the gender typing system Amelogenin was optimized for the amplification of hair DNA. Compared to commercial STR kits, this approach resulted in considerably higher success rates. 相似文献
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Let G be a connected graph. The resistance distance between any two vertices of G is defined as the net effective resistance between them if each edge of G is replaced by a unit resistor. The Kirchhoff index is the sum of resistance distances between all pairs of vertices in G. Zhou and Trinajstić (Chem Phys Lett 455(1–3):120–123, 2008) obtained a Nordhaus-Gaddum-type result for the Kirchhoff index by obtaining lower and upper bounds for the sum of the Kirchhoff
index of a graph and its complement. In this paper, by making use of the Cauchy-Schwarz inequality, spectral graph theory
and Foster’s formula, we give better lower and upper bounds. In particular, the lower bound turns out to be tight. Furthermore,
we establish lower and upper bounds on the product of the Kirchhoff index of a graph and its complement. 相似文献
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Martin J. Klein 《Physics in Perspective (PIP)》2010,12(3):307-337
In May 1918 Paul Ehrenfest received a monograph from Niels Bohr in which Bohr had used Ehrenfest’s adiabatic principle as
an essential assumption for understanding atomic structure. Ehrenfest responded by inviting Bohr, whom he had never met, to
give a talk at a meeting in Leiden in late April 1919, which Bohr accepted; he lived with Ehrenfest, his mathematician wife
Tatyana, and their young family for two weeks. Albert Einstein was unable to attend this meeting, but in October 1919 he visited
his old friend Ehrenfest and his family in Leiden, where Ehrenfest told him how much he had enjoyed and profited from Bohr’s
visit. Einstein first met Bohr when Bohr gave a lecture in Berlin at the end of April 1920, and the two immediately proclaimed
unbounded admiration for each other as physicists and as human beings. Ehrenfest hoped that he and they would meet at the
Third Solvay Conference in Brussels in early April 1921, but his hope was unfulfilled. Einstein, the only physicist from Germany
who was invited to it in this bitter postwar atmosphere, decided instead to accompany Chaim Weizmann on a trip to the United
States to help raise money for the new Hebrew University in Jerusalem. Bohr became so overworked with the planning and construction
of his new Institute for Theoretical Physics in Copenhagen that he could only draft the first part of his Solvay report and
ask Ehrenfest to present it, which Ehrenfest agreed to do following the presentation of his own report. After recovering his
strength, Bohr invited Ehrenfest to give a lecture in Copenhagen that fall, and Ehrenfest, battling his deep-seated self-doubts,
spent three weeks in Copenhagen in December 1921 accompanied by his daughter Tanya and her future husband, the two Ehrenfests
staying with the Bohrs in their apartment in Bohr’s new Institute for Theoretical Physics. Immediately after leaving Copenhagen,
Ehrenfest wrote to Einstein, telling him once again that Bohr was a prodigious physicist, and again expressing the hope that
he soon would see both of them in Leiden. 相似文献