Binding equilibrium of I− to serum albumin with resonance Rayleigh scattering

The binding equilibrium between l^? and human serum albumin (HSA) or bovine serum albumin (BSA) has been studied by means of the resonance Rayleigh scattering (RRS) and equilibrium dialysis. It has been found for the first time that RRS and multiple frequency scattering (MFS) are enhanced as the l^? binding to the HSA and BSA, but fluorescence quenches. The equilibrium dialysis results suggest that the binding of l^? to HSA and BSA fits a phase-distribution model other than Scatchard model, and that the order of magnitude of its phase-distribution constant was found to be 10⁴. It is most probable that Cl^? or other anion ions influence the binding of l^? by changing the ionic strength in the solution. The dialysis at different pH indicates that the binding mechanism is due to the electrostatic forces between the l^? and protonated basic amino-acid residues.     

Relationship between grain boundary diffusion in nanocrystals and amorphous microstructure

The effects of microstructure on diffusion behaviors of Ni in Zr₄₈Cu₃₆Ag₈Al₈ alloy were investigated by transmission electron microscope and secondary ion mass spectrum. The temperature dependence of Ni diffusivities in Zr₄₈Cu₃₆Ag₈Al₈ alloy with different states (metallic glass, nanocrystals and coarse crystals) has been measured in the temperature range of 693–723 K. The results show that diffusion behavior is microstructure dependence, and the relations between the microstructure and diffusion are discussed. The activity energy of the grain boundary diffusion in nanocrystals calculated by the Suzuoka solution is about 1.433 eV/atom. It is equivalent with that of metallic glass, which is attributed to the similar microstructure between the two. Copyright © 2016 John Wiley & Sons, Ltd.     

粗糙表面分维计算的立方体覆盖法

针对三角形棱柱表面积法和投影覆盖法在计算粗糙表面分形维数中存在的问题,提出了计算粗糙表面分维的立方体覆盖法,对计算结果进行了对比分析,并进一步对表面分维计算中的有关理论问题进行了分析,发现立方体覆盖法作为一种几何意义上的覆盖法,并计算结果比三角形棱柱表面积法和投影覆盖法更接近实际。     

Rapid Screening of Lipase Inhibitors from Ophiopogonis Radix Using High-Performance Thin Layer Chromatography by Two Step Gradient Elution Combined with Bioautographic Method

In this study, a high-performance thin layer chromatography (HPTLC) method by two step gradient elution with two mobile phases was developed for the simultaneous analysis of seven constituents in Ophiopogonis Radix. The chromatography was performed on silica gel 60 F₂₅₄ plate with dichloromethane-methanol-ethyl acetate-water (70:25:12:3, v/v/v/v) and dichloromethane-methanol (300:1, v/v) as the mobile phase for two step gradient elution. Then, the HPTLC profiles were observed after derivatization with 10% sulfuric acid in ethanol solution. The obtained HPTLC images were further analyzed by chemometric approaches and the samples could be clustered based on regions and/or growth years, which were two important factors affecting the constituents in Ophiopogonis Radix. Furthermore, five compounds including ophiopogonin D, ophiopojaponin C, ophiopogonin D’, ophiopogonin C’ and methylophiopogonanone B were screened as potential lipase inhibitors from Ophiopogonis Radix by the HPTLC-bioautographic method. The binding modes and interactions between the five compounds and lipase were further explored by molecular docking analysis. The developed HPTLC method could be used for quality control of Ophiopogonis Radix and screening of the potential lipase inhibitors.     

碾压混凝土坝渗流场与应力场耦合模型研究

针对碾压混凝土坝存在众多施工层面问题,提出了确定影响层厚度以及相应物理力学参数的方法;在此基础上,探讨并建立了碾压混凝土坝渗流场与应力场两场耦合模型,研制了相应的三维有限元程序．研究表明,结合试验和理论分析确定的碾压混凝土坝层面影响层的厚度、弹性模量和泊松比等参数更切合实际;提出的碾压混凝土坝渗流场与应力场两场耦合模型,能较好地体现两场耦合作用,开发的三维有限元模型能较充分反映碾压混凝土坝施工层面的特性．通过研究,为分析施工层面对碾压混凝土坝变化性态影响,以及了解大坝的应力和渗流的相互作用提供了科学依据．     

二异丙基酮定性定量方法的研究

采用色谱—质谱联用技术对酸—酸化反应制得的二异丙基酮化冷凝液进行了系统的定性,并且在色谱仪上利用外标法和面积归一化法对主要组分进行了定量方法的比较,得到了较好的定性定量结果。     

Anthraquinones from the Aerial Parts of Rubia cordifolia with Their NO Inhibitory and Antibacterial Activities

The present study aimed to identify the composition of the aerial parts of Rubia cordifolia L. A chemical investigation on the EtOAc extracts from the aerial parts of Rubia cordifolia resulted in the isolation of four new anthraquinones, namely Cordifoquinone A–D (1–4), along with 16 known anthraquinones. Their structures were elucidated on the basis of NMR and HR-ESIMS data. All isolates were assessed for their inhibitory effects on NO production in LPS-stimulated RAW 264.7 macrophage cells. Compounds 1, 3 and 10 exhibited significant inhibitory activities with IC₅₀ values of 14.05, 23.48 and 29.23 μmol·L⁻¹, respectively. Their antibacterial activities of four bacteria, Escherichia coli (ATCC 25922), Staphylococcus aureus subsp. aureus (ATCC 29213), Salmonella enterica subsp. enterica (ATCC 14028) and Pseudomonas aeruginosa (ATCC 27853), were also evaluated. Our results indicated that the antibacterial activity of these compounds is inactive.     

Negative and positive lateral shift of a light beam reflected from a grounded slab

We consider the lateral shift (LS) of a light beam reflecting from a dielectric slab backed by a metal. It is found that the LS of the reflected beam can be negative while the intensity of reflected beam is almost equal to the incident one under a certain condition. The explanation for the negativity of the LS is given in terms of the interference of the reflected waves from the two interfaces. It is also shown that the LS can be enhanced or suppressed under some other conditions. The numerical calculation on the LS for a realistic Gaussian-shaped beam confirms our theoretical prediction.     

Photonic generation of microwave signals using dual-wavelength single-longitudinal-mode fiber lasers

Photonic generation of microwave signals is demonstrated with a dual-wavelength single-longitudinal-mode erbium-doped fiber ring laser. Microwave signal with a frequency of 21.07 GHz is obtained by aligning the reflection band of a uniform fiber Bragg grating to the two transmission peaks within a fiber Bragg grating-based Fabry-Perot filter. Stable dual-wavelength single-longitudinal-mode operation is guaranteed by means of the combined filtering effect of a saturable absorber and the Fabry-Perot filter. This approach provides an effective solution to the photonic generation of high-frequency microwave signals.