Aberrantly truncated immature O-glycosylation in proteins occurs in essentially all types of epithelial cancer cells, which was demonstrated to be a common feature of most adenocarcinomas and strongly associated with cancer proliferation and metastasis. Although extensive efforts have been made toward the development of anticancer antibodies targeting MUC1, one of the most studied mucins having cancer-relevant immature O-glycans, no anti-MUC1 antibody recognises carbohydrates and the proximal MUC1 peptide region, concurrently. Here we present a general strategy that allows for the creation of antibodies interacting specifically with glycopeptidic neoepitopes by using homogeneous synthetic MUC1 glycopeptides designed for the streamlined process of immunization, antibody screening, three-dimensional structure analysis, epitope mapping and biochemical analysis. The X-ray crystal structure of the anti-MUC1 monoclonal antibody SN-101 complexed with the antigenic glycopeptide provides for the first time evidence that SN-101 recognises specifically the essential epitope by forming multiple hydrogen bonds both with the proximal peptide and GalNAc linked to the threonine residue, concurrently. Remarkably, the structure of the MUC1 glycopeptide in complex with SN-101 is identical to its solution NMR structure, an extended conformation induced by site-specific glycosylation. We demonstrate that this method accelerates dramatically the development of a new class of designated antibodies targeting a variety of “dynamic neoepitopes” elaborated by disease-specific O-glycosylation in the immunodominant mucin domains and mucin-like sequences found in intrinsically disordered regions of many proteins.We developed new class of designated antibodies targeting of “dynamic neoepitopes” elaborated by disease-specific O-glycosylation at the immunodominant mucin domains. 相似文献
Abstract— Thymine and uracil dimerized by ultraviolet irradiation (principally at 253 7 mμ) on filter paper and on quartz fiber sheet show a fairly good yield. These irradiation products were identical with the photodimers prepared from frozen aqueous solution. Thymine and uracil dimer in an aqueous solution reverted to the original pyrimidines by the ultraviolet irradiation with quantum yields of 0.65 and 0.50 respectively. Uracil dimer was rather labile towards hot alkaline solution. In neutral and acidic media, both dimers were quite stable. Photodimers of cytosine and isocytosine were not obtained. 相似文献
The guest- or solvent-induced assembly of a tetracarboxyl-cavitand 1 and a tetra(3-pyridyl)-cavitand 2 into a heterodimeric capsule 1.2 in a rim-to-rim fashion via four intermolecular CO(2)H.N hydrogen bonds has been investigated both in solution and in the solid state. In the (1)H NMR study, a 1:1 mixture of1a and 2a (R = (CH(2))(6)CH(3)) in CDCl(3) gave a mixture of various complicated aggregates, whereas this mixture in CDCl(2)CDCl(2) or p-xylene-d(10) exclusively produced the heterodimeric capsule 1a.2a. It was found that an appropriate 1,4-disubstituted-benzene is a suitable guest for inducing the exclusive formation of 1a.2a in CDCl(3). The ability of a guest to induce the formation of guest-encapsulating heterodimeric capsule, guest@(1a.2a), increased in the order p-ethyltoluene < 1-ethyl-4-methoxybenzene < or = 1-ethyl-4-iodobenzene < or = 1,4-dibromobenzene < 1-iodo-4-methoxybenzene < or= 1,4-dimethoxybenzene < or = 1,4-diiodobenzene. The (1)H NMR study revealed that a CH-halogen interaction between the inner protons of the methylene-bridge rims (-O-H(out)CH(in)-O-) of the 1a and 2a units and the halogen atoms of 1,4-dihalobenzenes and a CH-pi interaction between the methoxy protons of 1,4-dimethoxybenzene and the aromatic cavities of the 1a and 2a units play important roles in the formation of 1,4-dihalobenzene@(1a.2a) and 1,4-dimethoxybenzene@(1a.2a), respectively. A preliminary single-crystal X-ray diffraction analysis of guest@(1b.2b) (R = (CH(2))(2)Ph; guest = 1-iodo-4-methoxybenzene or p-xylene) confirmed that the guest encapsulated in 1b.2b is oriented with the long axis of the guest along the long axis of 1b.2b and that the iodo and the methoxy groups of the encapsulated 1-iodo-4-methoxybenzene are specifically oriented with respect to the cavities of the 2b and 1b units, respectively. 相似文献
A new toxin, named neosurugatoxin, was isolated from the toxic Japanese Ivory Shell and its structure was determined by X-ray crystallographic analysis. 相似文献
The manganese(III)-catalyzed aerobic oxidation of 2,4-piperidinediones was performed in the presence of alkenes at room temperature, producing 1-hydroxy-8-aza-2,3-dioxabicyclo[4.4.0]decan-7-ones in excellent yields. On the other hand, the 6-acetoxy-3-aza-7-oxabicyclo[4.3.0]nonan-2-ones were obtained by the oxidation of the 2,4-piperidinedione-3-carboxylates with manganese(III) acetate in the presence of alkenes at elevated temperature under an argon atmosphere. A similar oxidation using decarboxylated 2,4-piperidinediones produced the 2,3,6,7-tetrahydrofuro[3,2-c]pyridin-4(5H)-ones and/or 2,3,6,7-tetrahydrofuro[2,3-b]pyridin-4(5H)-ones in good yields. The structure determination and the decomposition reaction of the azabicyclic peroxides in acetic acid or acetic anhydride, and the reaction pathway were also described. 相似文献
A vinyl‐functionalized polyphosphate (PIOP) was synthesized by ring‐opening polymerization of 2‐isopropyl‐2‐oxo‐1,3,2‐dioxaphospholane and 2‐(2‐oxo‐1,3,2‐dioxaphosphoroyloxyethyl methacrylate) with triisobutylaluminum as an initiator. The number‐averaged molecular weight of the PIOP was 1.2 × 104. The average number of vinyl groups in the PIOP is 2.20. Transparent hydrogels were prepared by the radical polymerization of 2‐methacryroyloxyethyl phosphorylcholine with PIOP as a cross‐linking reagent. These hydrogels may have many applications in the biomedical field because of their biodegradability and biocompatibility.
The binding of a dimeric form of the 2-amino-1,8-naphthyridine derivative (naphthyridine dimer) to a human telomeric sequence, TTAGGG, was investigated by UV melting, CD spectra, and CSI-MS measurements. Both the 9-mer d(TTAGGGTTA) and the 15-mer d(TTAGGGTTAGGGTTA) showed apparent melting temperatures (T(m)) of 45.6 and 63.6 degrees C, respectively, in the presence of naphthyridine dimer (30 microM) in sodium cacodylate buffer (50 mM, pH 7.0) containing 100 mM NaCl. The CD spectra at 235 and 255 nm of the 9-mer increased in intensity accompanied with strong induced CDs at 285 and 340 nm upon complex formation with naphthyridine dimer. UV titration of the binding of naphthyridine dimer to the 9-mer at 320 nm showed a hypochromism of the spectra. A Scatchard plot of the data showed the presence of multiple binding sites with different association constants. Cold spray ionization mass spectrometry of the complex between naphthyridine dimer and the 9-mer clearly showed that one to three molecules of the ligand bound to the dimer duplex of the 9-mer. Telomeric repeat elongation assay showed that the binding of naphthyridine dimer to the telomeric sequence inhibits the elongation of the sequence by telomerase. 相似文献
Magnetic field effects (MFEs) on photocatalytic degradation of methylene blue (MB) solution over ZnO and TiO2 powders are investigated under static magnetic field up to 0.7 T with light irradiation by ultraviolet (UV) light emitting
diode. The UV–visible-near-infrared spectrometer is used to monitor the MB concentrations. The positive MFE is observed for
ZnO, while the negative MFE for TiO2, and both MFEs are increased with the increase in the magnetic field applied. By increasing the settling time (the time interval
between the preparation of MB solution and the powder dispersion into the MB solution), the photodegradation abilities under
MFEs are decreased for both the catalysts. The cause of MFE is discussed in terms of dissolved oxygen in the MB solution and
magnetic adsorption of the constituent molecules. 相似文献