Development of NIR fluorescent dyes based on Si-rhodamine for in vivo imaging

We have developed a series of novel near-infrared (NIR) wavelength-excitable fluorescent dyes, SiR-NIRs, by modifying the Si-rhodamine scaffold to obtain emission in the range suitable for in vivo imaging. Among them, SiR680 and SiR700 showed sufficiently high quantum efficiency in aqueous media. Both antibody-bound and free dye exhibited high tolerance to photobleaching in aqueous solution. Subcutaneous xenograft tumors were successfully visualized in a mouse tumor model using SiR700-labeled anti-tenascin-C (TN-C) antibody, SiR700-RCB1. SiR-NIRs are expected to be useful as labeling agents for in vivo imaging studies including multicolor imaging, and also as scaffolds for NIR fluorescence probes.     

Detection of influenza virus using a lateral flow immunoassay for amplified DNA by a microfluidic RT-PCR chip

Influenza virus RNA was amplified by a continuous-flow polydimethylsiloxane microfluidic RT-PCR chip within 15-20 min. The amplified influenza virus RNA was observed with the naked eye, as the red color at the test line, using a lateral flow immunoassay within 1 min.     

A weighted independent even factor algorithm

An even factor in a digraph is a vertex-disjoint collection of directed cycles of even length and directed paths. An even factor is called independent if it satisfies a certain matroid constraint. The problem of finding an independent even factor of maximum size is a common generalization of the nonbipartite matching and matroid intersection problems. In this paper, we present a primal-dual algorithm for the weighted independent even factor problem in odd-cycle-symmetric weighted digraphs. Cunningham and Geelen have shown that this problem is solvable via valuated matroid intersection. Their method yields a combinatorial algorithm running in O(n ³ γ +? n ⁶ m) time, where n and m are the number of vertices and edges, respectively, and γ is the time for an independence test. In contrast, combining the weighted even factor and independent even factor algorithms, our algorithm works more directly and runs in O(n ⁴ γ?+?n ⁵) time. The algorithm is fully combinatorial, and thus provides a new dual integrality theorem which commonly extends the total dual integrality theorems for matching and matroid intersection.     

FM-AFM imaging of a commercial polyethylene film immersed in n-dodecane

The subnanometer topography of a partially crystalline polyethylene film was observed in liquid n-dodecane using a frequency-modulation atomic force microscope. Locally ordered structures were found and assigned to a (100) facet of crystalline domains.     

Stable surface termination on vicinal 6H-SiC(0 0 0 1) surfaces

Ordered nanofacet structures on vicinal 6H-SiC(0 0 0 1) surfaces, consisting of pairs of a (0 0 0 1) basal plane and a facet, are investigated in terms of stable surface stacking of the (0 0 0 1) basal planes. The surface termination of S3 (or S3*), i.e., ABC (or A*C*B*), was suggested by a structural model based on quantized step-bunching, which typically gives a one-unit-cell bunched step configuration at the facet. Here, we evaluate the surface termination at basal planes covered with a layer of silicon oxynitride by means of quantitative low-energy electron diffraction (LEED) analysis combined with scanning tunneling microscopy (STM), and show the validity of the structural model proposed.     

Note on upper bound graphs and forbidden subposets

In the upper bound graph of a poset P, the vertex set is V(P) and xy is an edge if there exists an m∈V(P) with x,y≤_Pm. We show some characterizations on split upper bound graphs, threshold upper bound graphs and difference upper bound graphs in terms of m-subposets and canonical posets.     

Wave field restoration using focal-depth extension techniques under dynamic hollow-cone illumination

Wave field restoration has been achieved based on focal-depth extension under dynamic hollow-cone illumination using an optical microscope. Two kinds of focal-depth extension techniques, such as moving focus averaging and an objective annular aperture, were compared and their effectiveness for transmission electron microscopy applications is discussed. Both techniques enabled resolution enhancement at the same level and effectively obtained aberration-free phase and amplitude images separately by canceling out the rotationally invariant component of system wave aberrations, such as spherical aberration. The influence of nonlinear interference on image contrast was confirmed as significant by using a figure 8 shaped spatial frequency filter.     

An application of oxide and silver electrode on the BaO-doped Bi2O3 electrolyte

Oxide and silver paste were applied on the BaO-doped Bi₂O₃ electrolyte and their behavior was studied as a function of temperature and oxygen partial pressure. Interface resistance of most oxide/electrolyte were of the same order of magnitude with those of Ag paste/electrolyte in air (300–500°C). A high electrode capacitance of (0.8–1.7)×10^?2 F/cm² was observed for the silver electrode at 450°C in the P_O2 region of 1–10^?5 atm.     

Polymer electrolyte plasticized with PEG-borate ester having high ionic conductivity and thermal stability

We have focused on the PEG-borate ester as a new type of plasticizer for solid polymer electrolyte composed of poly(ethyleneglycol) methacrylate (PEGMA) and lithium bis-trifluoromethanesulfonimide (LiTFSI). The PEG-borate ester shows good thermal stability and high flash point. Ionic conductivity of the polymer electrolyte increases with increasing amount of the PEG-borate ester and exhibits values greater than 10⁻⁴ S cm⁻¹ at 30 °C and 10⁻³ S cm⁻¹ at 60 °C. Furthermore, PEG-borate ester has three EO chains whose lengths are variable, and various ionic conductivities are expected to depend on EO chain length. As a result, polymer electrolyte containing the PEG-borate ester whose EO chain length is n=3 shows highest ionic conductivity. Furthermore, polymer electrolytes containing PEG-borate esters show excellent thermal and electrochemical stability. The electrolytes are thermally stable up to 300 °C and electrochemically up to 4.5 V vs. Li⁺/Li.