Polypeptide-synthetic polymer hybrids, 1. Miscibility of poly(vinyl alcohol) with poly(sodium α,β-D,L-aspartate)

The present paper describes materials of polypeptide-commodity polymer hybrids from poly(vinyl alcohol) (PVA) and poly(sodium α,β-D ,L -aspartate) ( 1 ). Miscible blend films of polypeptide 1 and PVA were prepared by the solvent-cast method from a homogeneous aqueous solution. Differential scanning calorimetry. Fourier transform infrared, and scanning electron microscopy combined with energy dispersive X-ray spectroscopy (EDX) were used to investigate the blends. It was revealed that 1 and PVA are miscible in a wide range of compositions.     

New estimation methods of erase band width and track-edge noise in perpendicular magnetic recording

In order to realize higher track density, improving recording resolution and noise at track edge is one of the crucial issues. It has been considered that the erase band (EB), which appears at track-edge, can suppress the cross-talk noise from the adjacent track. On the other hand, the EB consists of a randomly magnetized area that may emanate track-edge noise. In this study, we estimated the accurate EB width by a newly proposed technique and derived analytical formula of the 747 curve with large EB noise. As a result, we found that the peak of track-edge noise was outside the EB and the EB was enough of a noise source at track edge and may be obstructive to the improvement of track density. A bump of 747 curve was produced in the case of little noise of EB. There is a close relationship between the shape of the 747 curve and the EB noise.     

Self-stability of a simple walking model driven by a rhythmic signal

In this paper, we analyzed the dynamic properties of a simple walking model of a biped robot driven by a rhythmic signal from an oscillator. The oscillator receives no sensory feedback and the rhythmic signal is an open loop. The simple model consists of a hip and two legs that are connected at the hip. The leg motion is generated by a rhythmic signal. In particular, we analytically examined the stability of a periodic walking motion. We obtained approximate periodic solutions and the Jacobian matrix of a Poincaré map by the power-series expansion using a small parameter. Although the analysis was inconclusive when we used only the first order expansion, by employing the second order expansion it clarified the stability, revealing that the periodic walking motion is asymptotically stable and the simple model possesses self-stability as an inherent dynamic characteristic in walking. We also clarified the stability region with respect to model parameters such as mass ratio and walking speed.     

In situ Raman imaging of osteoblastic mineralization

Hydroxyapatite (HA) is synthesized at early stages of bone formation by osteoblasts. Nondestructive observation of early stages of osteoblastic mineralization provides crucial information for biological mechanism of bone formation. Raman microscopy serves as an ideal tool to observe the osteoblastic mineralization process because it shows the chemical information of the sample at a minimally invasive level. In addition, HA is a marker for osteoblastic mineralization, and HA Raman signal is strong enough to identify mineralized spots in osteoblasts. In this research, we visualized the distribution of HA in cultured mouse osteoblasts by Raman imaging and observed the location of the mineralized spots in the culture. We monitored HA Raman signal from osteoblast culture for 3 days after administrating the osteogenic differentiation medium and observed Raman signal associated with HA. We identified mineralized spots of KUSA‐A1 by Raman imaging constructed from the distribution of HA Raman signal. We successfully visualized the distribution of the mineralized spots in the culture of KUSA‐A1. We compared our Raman images with Alizarin red S staining assay, which was a conventional method to evaluate the mineralization process. Raman imaging of the KUSA‐A1 culture visualized the mineralized spots more accurately than Alizarin red S staining assay. Raman imaging of HA serves as a powerful tool to identify the mineralized spots in an in vitro culture of osteogenic lineage cells. Copyright © 2014 John Wiley & Sons, Ltd.     

Ruthenium Complexes with Hydrophobic Ligands That Are Key Factors for the Optical Imaging of Physiological Hypoxia

The phosphorescence emission of ruthenium complexes was applied to the optical imaging of physiological hypoxia. We prepared three complexes with hydrophobic substituents on the phenanthroline ligand and characterized their emission, which was quenched by molecular oxygen. Among the complexes synthesized in this study, a pyrene chromophore‐linked ruthenium complex, Ru‐Py, exhibited optimal properties for the imaging of hypoxia; the prolonged lifetime of the triplet excited state of the ruthenium chromophore, which was induced by efficient energy distribution and transfer from the pyrene unit, provided the highest sensitivity towards molecular oxygen. The introduction of hydrophobic pyrene increased the lipophilicity of the complex, leading to enhanced cellular uptake. Consequently, the bright phosphorescence of Ru‐Py was seen in the cytoplasm of viable hypoxic cells, whereas the signal from aerobic cells was markedly weaker. Thus, we could clearly discriminate between hypoxic and aerobic cells by monitoring the phosphorescence emission. Furthermore, Ru‐Py was applied to optical imaging in live mice. An intramuscular injection of Ru‐Py successfully visualized ischemia‐based hypoxia, which was constructed by leg banding.     

Metal-free halogenation of arylboronate with N-halosuccinimide

Efficient bromination and chlorination of aryl pinacol boronates were accomplished without the addition of metal reagent. The reaction proceeded efficiently with electron-rich arylboronates or heteroarylboronates in DMF or acetonitrile, to afford mono-, di-, or trihalogenated aryl pinacol boronates.     

Beta-decay study of Tz = - 2 proton-rich nucleus 24Si

$ \beta$ -decay spectroscopy on a T _z = - 2 proton-rich nucleus ²⁴Si was performed. The decay scheme of ²⁴Si was reconstructed by the $ \beta$ -delayed $ \gamma$ -ray and proton measurements. Two $ \beta$ branches to the bound 1₁ ⁺ and 1₂ ⁺ states in ²⁴Al were observed for the first time. The observation of the allowed transition firmly established the spin-parity assignment for the 1₂ ⁺ states. The branching ratios to the 1₁ ⁺ and 1₂ ⁺ states were determined to be 31(4)% and 23.9(15)%, respectively. The branching ratios to three unbound states in ²⁴Al including a new level at 6.735MeV were also determined for the first time. The level structure of ²⁴Al was compared with its mirror nucleus ²⁴Na . The Thomas-Ehrman shift on the 1₂ ⁺ state indicates s -wave dominance in the state as well as a characteristic behavior of the weakly bound s -wave proton in ²⁴Al .     

Biodegradable polymers based on renewable resources. IX. Synthesis and degradation behavior of polycarbonates based on 1,4:3,6‐dianhydrohexitols and tartaric acid derivatives with pendant functional groups

Novel polycarbonates, with pendant functional groups, based on 1,4:3,6‐dianhydrohexitols and L ‐tartaric acid derivatives were synthesized. Solution polycondensations of 1,4:3,6‐dianhydro‐bis‐O‐(p‐nitrophenoxycarbonyl)hexitols and 2,3‐di‐O‐methyl‐L ‐threitol or 2,3‐O‐isopropylidene‐L ‐threitol afforded polycarbonates having pendant methoxy or isopropylidene groups, respectively, with number average molecular weight (M_n) values up to 3.6₁ × 10⁴. Subsequent acid‐catalyzed deprotection of isopropylidene groups gave well‐defined polycarbonates having pendant hydroxyl groups regularly distributed along the polymer chain. Differential scanning calorimetry (DSC) demonstrated that all the polycarbonates were amorphous with glass transition temperatures ranging from 57 to 98 °C. Degradability of the polycarbonates was assessed by hydrolysis test in phosphate buffer solution at 37 °C and by biochemical oxygen demand (BOD) measurements in an activated sludge at 25 °C. In both tests, the polycarbonates with pendant hydroxyl groups were degraded much faster than the polycarbonates with pendant methoxy and isopropylidene groups. It is noteworthy that degradation of the polycarbonates with pendant hydroxyl groups was remarkably fast. They were completely degraded within only 150 min in a phosphate buffer solution and their BOD‐biodegradability reached nearly 70% in an activated sludge after 28 days. The degradation behavior of the polycarbonates is discussed in terms of their chemical and physical properties. © 2005 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 43: 3909–3919, 2005