Multi-wavelength laser sensor for intruder detection and discrimination

An intruder detection and discrimination sensor with improved optical design is developed using lasers of different wavelengths to demonstrate the concept of discrimination over a distance of 6 m. A distinctive feature of optics is used to provide additional transverse laser beam scanning. The sample objects used to demonstrate the concept of discrimination over a distance of 6 m are leaf, bark, black fabric, PVC, wood and camouflage material. A camouflage material is chosen to illustrate the discrimination capability of the sensor. The sensor utilizes a five-wavelength laser combination module, which sequentially emits identically-polarized laser light beams along one optical path. A cylindrical quasi-optical cavity with improved optical design generates multiple laser light beams for each laser. The intensities of the reflected light beams from the different spots are detected using a high speed area scan image sensor. Object discrimination and detection is based on analyzing the Gaussian profile of reflected light at the different wavelengths. The discrimination between selected objects is accomplished by calculating four different slopes from the objects' reflectance spectra at the wavelengths 473 nm, 532 nm, 635 nm, 670 nm and 785 nm. Furthermore, the camouflage material, which has complex patterns within a single sample, is also detected and discriminated over a 6 m range by scanning the laser beam spots along the transverse direction.     

Synthesis and characterization of piperazine containing polyaspartimides blended polysulfone membranes for fuel cell applications

Journal of Solid State Electrochemistry - A new polyaspartimide was synthesized via a Michael addition reaction of an aromatic bismaleimide (BMI) with aminoethylpiperazine (AEP) at 1:1 molar ratio....     

Detection of Bacterial Neutral Ceramidase in Diabetic Foot Ulcers with an Optimized Substrate and Chemoenzymatic Probes

Ceramidases (CDases) are important in controlling skin barrier integrity by regulating ceramide composition and affording downstream signal molecules. While the functions of epidermal CDases are known, roles of neutral CDases secreted by skin-residing microbes are undefined. Here, we developed a one-step fluorogenic substrate, S-B , for specific detection of bacterial CDase activity and inhibitor screening. We identified a non-hydrolyzable substrate mimic, C6 , as the best hit. Based on C6 , we designed a photoaffinity probe, JX-1 , which efficiently detects bacterial CDases. Using JX-1 , we identified endogenous low-abundance PaCDase in a P. aeruginosa monoculture and in a mixed skin bacteria culture. Harnessing both S-B and JX-1 , we found that CDase activity positively correlates with the relative abundance of P. aeruginosa and is negatively associated with wound area reduction in clinical diabetic foot ulcer patient samples. Overall, our study demonstrates that bacterial CDases are important regulators of skin ceramides and potentially play a role in wound healing.