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Ukonaho T Rantanen T Jämsen L Kuningas K Päkkilä H Lövgren T Soukka T 《Analytica chimica acta》2007,596(1):106-115
Research in the field of immunoassays and labels used in the detection has been recently focused on particulate reporters, which possess very high specific activity that excludes the label as a sensitivity limiting factor. However, the large size and shape of the particulate labels may produce additional problems to immunoassay performance. The aim of this work was to study with two identical non-competitive two-site immunoassays whether up-converting phosphor (UCP) particles are comparable in performance with europium(III) chelate-dyed nanoparticles as particulate labels. In addition we strived to verify the common assumption of the photostability of up-converting phosphor particles supporting their potential applicability in imaging. Detection limits in two-site immunoassay for free prostate-specific antigen (free-PSA) were 0.53 ng L−1 and 1.3 ng L−1 using two different up-converting phosphors and 0.16 ng L−1 using europium(III) nanoparticle. Large size distribution and non-specific binding of up-converting phosphor particles caused assay variation in low analyte concentrations and limited the analytical detection limit. The non-specific binding was the major factor limiting the analytical sensitivity of the immunoassay. The results suggests the need for nanoscaled and uniformely sized UCP-particles to increace the sensitivity and applicability of up-converting phosphor particles. Anti-Stokes photoluminescence of up-converting phosphor particles did not photobleach when measured repeatedly, on the contrary, the time-resolved fluorescence of europium nanoparticles photobleached relatively rapidly. 相似文献
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Ras RH Ruotsalainen T Laurikainen K Linder MB Ikkala O 《Chemical communications (Cambridge, England)》2007,(13):1366-1368
Long hollow inorganic nanoparticle nanotubes have been synthesized by templating of block copolymer electrospun fibers with clay mineral platelets followed by interlinking of the platelets using condensation reactions. 相似文献
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Katri Huitu Ritva Kinnunen Jari Laamanen Sami Lehti Sourov Roy Tapio Salminen 《The European Physical Journal C - Particles and Fields》2008,58(4):591-608
In grand-unified theories (GUT), non-universal boundary conditions for the gaugino masses may arise at the unification scale
and may affect the observability of the neutral MSSM Higgs bosons (h/H/A) at the LHC. The implications of such non-universal gaugino masses are investigated for Higgs boson production in the SUSY
cascade decay chain
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produced in pp interactions. In the singlet representation with universal gaugino masses only the light Higgs boson can be produced in this
cascade with the parameter region of interest for us, while with non-universal gaugino masses heavy neutral MSSM Higgs boson
production may dominate. The allowed parameter space in the light of the WMAP constraints on the cold dark-matter relic density
is investigated in the above scenarios for gaugino mass parameters. We also demonstrate that combination of representations
can give the required amount of dark matter in any point of the parameter space. In the non-universal case we show that heavy
Higgs bosons can be detected in the cascade studied in parameter regions with the WMAP preferred neutralino relic density. 相似文献
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We explore possible signatures for right-handed neutrinos in a TeV scale B-L extension of the standard model at the Large Hadron Collider. The studied four lepton signal has a tiny standard model background. We find the signal experimentally accessible at the LHC for the considered parameter regions. 相似文献
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Chromatographia - The selectivity of silicone phases OV-101, OV-17 and OV-225 with respect to the retention indices and thermodynamic functions of solution (relative molar enthalpies, entropies and... 相似文献
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Summary The retention indices (I) and their structural increments (l/°C, H, l) of C6–C14 n-alkenes on polyphenyl ether (PFE) and C12–C14 n-alkenes on polyethylene glycol 4000 (PEG) at 20–160 °C have been determined and correlated with the molecular structure of the isomers. A number of common regularities in gas chromatographic behaviour on these liquid phases and squalane (SQ) [1–3] have been found. On PFE the elution order of isomeric n-alkenes is similar to that on SQ (except C8–C10 1-alkenes and cis-4-tetradecene), for pairs with cis- and trans-configuration it depends on the column temperature, position of double bond and the number of carbon atoms (n) in the molecule. On PEG the cis- are always retained longer than the corresponding trans-alkenes. The linear relationships between 1 and n on PFE and PEG are given in equation form. 相似文献