Differential proteome analysis of colon carcinoma cell line SW480 after reconstitution of the tumour suppressor Smad4

The tumour suppressor gene Smad4 is frequently inactivated in gastrointestinal carcinomas. Smad4 plays a pivotal role in transducing signals of the transforming growth factor-β (TGF-β) superfamily of proteins. Inactivation of Smad4 seems to occur late during tumour progression when tumours acquire invasive and metastatic properties. Identification of proteins directly or indirectly regulated by Smad4 would, therefore, ease the future design of new diagnostic and therapeutic strategies for gastrointestinal carcinoma. We have used human colon carcinoma cell line SW480 stably transfected with Smad4 as an in-vitro model system to identify Smad4-regulated proteins by applying two-dimensional gel electrophoresis (2DE) then MALDI-PMF/PFF-MS. We identified a total of 47 protein species with a Smad4-dependent expression. From the functions of the candidate proteins we obtained new insights into Smad4’s participation in processes, for example apoptosis, differentiation, and proliferation.     

Complex Inorganic/Organic Core-Shell Particles by an Inverse Emulsion Technique

Two synthetic approaches to modify the surface of inorganic particles are presented. In the first approach the inorganic particles are prepared in-situ in a confined space in inverse emulsions. The used amphiphilic statistical copolymers act not only as emulsifiers, but they also hydrophobize the remaining inorganic particles after the precipitation. This approach represents a versatile method to obtain various inorganic nanoparticles as well as more complex inorganic materials like core-multiple shell and perovskite-based nanoparticles. The second procedure uses preformed inorganic particles, as an aqueous dispersion, to modify them with surface active amphiphilic copolymers in a multicomponent solvent system. This method turns out to be a simple but highly efficient method to modify preformed inorganic nanoparticles. The particles are characterized by SEM, TEM and dynamic light scattering. The modified inorganic nanoparticles are suitable to be homogenously incorporated into a polymer matrix to form transparent nanocomposite materials.     

A unification of two refinements of Euler’s partition theorem

We obtain a unification of two refinements of Euler’s partition theorem respectively due to Bessenrodt and Glaisher. A specialization of Bessenrodt’s insertion algorithm for a generalization of the Andrews-Olsson partition identity is used in our combinatorial construction.     

Comparison of in-tube sorptive extraction techniques for non-polar volatile organic compounds by gas chromatography with mass spectrometric detection

A commercial in-tube sorptive extraction device, known as solid-phase dynamic extraction (SPDE), has been evaluated for the extraction of non-polar volatile aromatic analytes from aqueous solutions in both headspace and liquid injection modes. An automated sampler is used with a gas-tight 2.5 ml syringe equipped with a special needle that is coated on the inside with a non-polar extraction phase. After absorption onto the phase, the analytes were thermally desorbed directly into a GC-MS system. The technique was evaluated for the determination of furan, benzene and toluene. The sensitivity for toluene was greatly improved on using SPDE compared to static headspace. A slight increase in sensitivity was observed for benzene but none for determination of furan. Estimated limits of detection ranged from 0.2 to 2 microg/l.     

A performance study of radio-opaque personal protective fabrics for the reduction of transmittance of gamma-rays and neutrons

Commercial radio-opaque combat (CRC) fabrics, for incorporation into personal protective equipment used by first responders and armed forces, are marketed as having the ability to provide a level of protection against specific types of radiation. For a CRC material, a standard combat uniform and a multi-layered chemical, biological, radiological, nuclear (CBRN) protective material, the present work examines chemical composition and radiation protection against gamma-rays and neutron fluxes. Significant reduction in gamma-ray transmittance occurs only for the CRC fabric (46–514 keV) with gamma-ray attenuation coefficients of 3.10 to <0.10 cm² g⁻¹. Reduction in neutron transmittance, for all three fabrics, could not be assessed with certainty as the measured transmittance was obscured by large statistical uncertainties.     

Extending shelling orders and a hierarchy of functions of unimodal simple polytopes

Let P be a simplicial d-polytope with n facets ((d − 1)-dimensional faces) in R^d. A shelling of P is an ordering of the facets of P such that the intersection of each facet F with the union of all facets that precede it the ordering is a nonempty union of (d − 2)-faces of F. The following open question was raised by Tverberg and is recorded in [4]. Suppose for some k < n, there is an ordering of k of the facets of P so that the intersection of each of these facets with the union of all of the facets that precede it in the ordering is a nonempty union of (d − 2)-faces. Can this initial “segment” be extended to a shelling of all the facets? This question is open even in the case that P is the dual of the d-dimensional hypercube. The question in this case has resurfaced several times since G. Danaraj and V. Klee (1978) in a variety of forms. It is related to the hierarchies of completely unimodal pseudo-Boolean functions studied in P.L. Hammer et al. (1988), the author (1988) and D. Wiedemann (1986). (A pseudo-Boolean function is a function mapping the vertices of the d-dimensional hypercube into the reals). In this paper, the hierarchies are compared and combined. This hierarchy is then extended to general simple polytopes, and the relationship to the above open question is explained.     

Normals in a Minkowski Plane

In this paper we will establish bounds on the average number of normals through a point in a convex body in a Minkowski plane for certain classes of convex bodies. Also, a related Euler relation is discussed.