Biostimulation of HeLa cells by low-intensity visible light

Summary The action of low-intensity red light with λ=633 nm (a He−Ne laser, a filament lamp with light filters a dye-laser pumped by a Cu laser) on the intensity of nucleic-acid synthesis in HeLa cells 1.5 hours after irradiation has been studied. It has been shown that the DNA synthesis is stimulated similarly after irradiation both by the He−Ne laser and by an ordinary source. The RNA synthesis intensity does not alter in both cases. A high-repetition-rate radiation at λ=633 nm acts in the opposite manner: it stimulates the RNA synthesis and the DNA synthesis remains constant. The action spectra of the DNA and RNA synthesis stimulation by continuous light in the range (570÷693) nm are presented.

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Riassunto è stata studiata l'azione della luce rossa a bassa intensità con λ=633nm (un laser a He−Ne, una lampadina a filamento con filtri per la luce, un laser a colorante pompato da un laser a Cu) sull'intensità della sintesi degli acidi nucleici in cellule HeLa un'ora e mezzo dopo irradiazione. Si è mostrato che la sintesi del DNA è stimolata in modo simile dopo irradiazione sia con laser a He−Ne che da una sorgente ordinaria. La sintesi del RNA non è alterata in entrambi i casi. Una radiazione ad alta frequenza di ripetizione a λ=633 nm agisce in maniera opposta: stimola la sintesi del RNA, mentre la sintesi del DNA resta costante. Si forniscono gli spettri d'azione della sintesi di DNA ed RNA mediante luce continua nell'intervallo da 570 a 693 nm.

     

IRRADIATION OF HUMAN LYMPHOCYTES WITH He-Ne LASER INCREASES THE BLAST Ⅰ TRANSFORMATION CAUSED BY PHYTOHEMAGGLUTSNIN

The irradiation of human lymphocytes with a He-Ne laser(632.8nm,56J/m~2) elevated the DNA synthesis levol in phytohemagglutinin-treated cells,but didnot itself cause the blasttransformation.The boosting effect of irradition was especiallypronounced in case of suboptimal PHA concentrations(0.5and 1μg/ml).     

Irradiation of human lymphocytes with He-Ne laser dose not express the interleukin-2 receptors

The irradiation of human lymphocytes with a He-Ne laser(632.8nm,56J/cm~2)does not express interleukin-2 receptors on membrances of T-lymphocytes     

Weak toroidalization over non-closed fields

We prove that any dominant morphism of algebraic varieties over a field k of characteristic zero can be transformed into a toroidal (hence monomial) morphism by projective birational modifications of source and target. This was previously proved by the first and third author when k is algebraically closed. Moreover we show that certain additional requirements can be satisfied.     

Hard Lefschetz theorem for simple polytopes

McMullen’s proof of the Hard Lefschetz Theorem for simple polytopes is studied, and a new proof of this theorem that uses conewise polynomial functions on a simplicial fan is provided.     

Biostimulation of HeLa cells by low-intensity visible light. V. Stimulation of cell proliferationin vitro by He−Ne Laser irradiation

Summary Autoradiographic experiments, performed with monolayer HeLa cells, show that the irradiation with He-Ne laser (D=100 J/m²) causes an increase in the number of S-phase cells connected with enhanced G₁-S transition of a part of the population, as well as an increase in the grain count on the labelled nuclei connected with an enhancement of DNA synthesis in Sphase cells. The irradiation influences the proliferation rate of various subpopulations not in equal degree, as shown analysing the clone size distribution after the irradiation (D=10, 10², 10³J/m²). The stimulative effect of irradiation is most noticeable on the proliferation activity of the slowly growing subpopulations.

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Riassunto Esperimenti autoradiografici, attuati su un monostrato di cellule HeLa, mostrano che l’irraggiamento con un laser He-Ne (D=100J/m²) causa l’aumento del numero delle cellule in fase S connesso con un aumento della transizione G₁-S di una parte della popolazione, nonché l’aumento dei granuli nei nuclei marcati, connesso con un aumento della sintesi del DNA nelle cellule in fase S. L’irraggiamento influenza la velocità di proliferazione di varie sottopopolazioni non in misura uguale a quella mostrata analizzando la distribuzione delle dimensioni del clone dopo l’irraggiamento (D=10, 10², 10³J/m²). L’effetto stimolatorio dell’irraggiamento si nota di piú sull’attività proliferativa delle sottopopolazioni in crescita lenta.

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Резюме Авторадиографические эксперименты, проведенные с монослоем клеток HeLa показали, что облучение He-Ne лазером (D=100 ДжМ²) приводит к увеличению количества клеток вS-фазе, связанному с ускорением перехода части популяции изG ₁ вS-фазу. Также отмечено увеличение количства зерен в меченых ядрах, связанное с ускорением синтеза ДНК в клеткахS-фазы. Облучение влияет на скорость пролиферации различных субппопуляций в разиой степени как показал анализ распределения размеров клонов после облученияD=10, 10², 10³ Дж/М². Эффект стимуляции пролиерации наиболее заметен в случае медленно растуших субпопуляций.

     

Semistable Reduction in Characteristic Zero for Families of Surfaces and Threefolds

We consider the problem of extending the semistable reduction theorem of [KKMS] from the case of one-parameter families of varieties to families over a base of arbitrary dimension. Following [KKMS], semistable reduction of such families can be reduced to a problem in the combinatorics of polyhedral complexes [AK]. In this paper we solve it in the case when the relative dimension of the morphism is at most three, i.e., for families of surfaces and threefolds. Received March 18, 1998, and in revised form October 7, 1998.     

Synthesis and characterization of gaseous fuel from Jatropha oil through catalytic reactor and its performance in DI diesel engine

Journal of Thermal Analysis and Calorimetry - In this research, nanofluid heat transfer enhancement in a pipe by means of twisted tape with alternate axis is presented. Finite volume method is...     

溴化四丁基铵-碘化钾-水体系中Bi(Ⅲ)与Zr(Ⅳ)、Ce(Ⅲ)、U(Ⅵ)和Mg(Ⅱ)的浮选分离

在水溶液中,Bi(Ⅲ)与溴化四丁基铵和KI形成不溶于水的三元缔合物BiI-4·TBAB+,此三元缔合物沉淀浮于盐水相上层形成界面清晰的液-固两相。当溶液中溴化四丁基铵和KI的浓度分别为5.0×10-4mol/L和3.0×10-3mol/L,pH=3时,Bi(Ⅲ)可与Zr(Ⅳ)、Ce(Ⅲ)、U(Ⅵ)和Mg(Ⅱ)定量分离,Bi(Ⅲ)的浮选率达到100%。对合成水样中Bi(Ⅲ)的分离和测定,效果良好。该方法具有选择性高,操作简便和不污染环境等特点,在微量铋的分离和富集分析中有一定的实用价值。     

含2-N,N-二甲氨基嘧啶环与噻吩环结构的共轭聚合物的合成及其性能

合成了一种新型单体2-N,N-二甲氨基-4,6-二氯嘧啶(ADNP).以二氯-1,3-双(二苯基磷)丙烷基镍(Ⅱ)作催化剂,通过2,5-二溴噻吩格氏试剂与该单体共聚得到了一种新型共轭聚合物,聚[(2-N,N-二甲胺基嘧啶-4,6-二)-2,5-噻吩](PThPm).采用FT-IR、1H-NMR等测试手段对新型单体与共聚物的结构进行了表征,并用UV-Vis、荧光光谱、X射线衍射、循环伏安、热重分析(TGA)等测试方法对单体和共聚物进行了性能分析.结果表明:共聚物在420 nm处出现UV-Vis吸收峰,在528 nm处出现荧光发射峰;共聚物的DMF溶液存在酸致变色现象,加入CF3COOH和CH3SO3H后,溶液由淡绿黄色转变为淡红色,UV-Vis吸收峰发生红移;PThPm共聚物有一定的结晶性和热稳定性;在-1.8～ 1.8 V之间出现电致变色现象,电极周围的溶液颜色从深蓝色变成深红色.