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151.
The biosciences require the development of methods that allow a non-invasive and rapid investigation of biological systems.
In this aspect, high-end imaging techniques allow intravital microscopy in real-time, providing information on a molecular
basis. Far-field fluorescence imaging techniques are some of the most adequate methods for such investigations. However, there
are great differences between the common fluorescence imaging techniques, i.e., wide-field, confocal one-photon and two-photon
microscopy, as far as their applicability in diverse bioscientific research areas is concerned. In the first part of this
work, we briefly compare these techniques. Standard methods used in the biosciences, i.e., steady-state techniques based on
the analysis of the total fluorescence signal originating from the sample, can successfully be employed in the study of cell,
tissue and organ morphology as well as in monitoring the macroscopic tissue function. However, they are mostly inadequate
for the quantitative investigation of the cellular function at the molecular level. The intrinsic disadvantages of steady-state
techniques are countered by using time-resolved techniques. Among these fluorescence lifetime imaging (FLIM) is currently
the most common. Different FLIM principles as well as applications of particular relevance for the biosciences, especially
for fast intravital studies are discussed in this work.
相似文献
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On NaCl(100)/Cu(111) an interface state band is observed that descends from the surface-state band of the clean copper surface. This band exhibits a Moiré-pattern-induced one-dimensional band gap, which is accompanied by strong standing-wave patterns, as revealed in low-temperature scanning tunneling microscopy images. At NaCl island step edges, one can directly see the refraction of these standing waves, which obey Snell's refraction law. 相似文献
154.
I examine the publications of Carl Neumann (1832–1925) on electrodynamics, which constitute a major part of his work and which illuminate his approach to mathematical physics. I show how Neumann contributed to physics at an important stage in its development and how his work led to a polemic with Hermann Helmholtz (1821–1894). Neumann advanced and extended the ideas of the Königsberg school of mathematical physics. His investigations were aimed at founding a mathematically exact physical theory of electrodynamics, following the approach of Carl G.J. Jacobi (1804–1851) on the foundation of a physical theory as outlined in Jacobis lectures on analytical mechanics. Neumanns work also shows how he clung to principles that impeded him in appreciating and developing new ideas such as those on field theory that were proposed by Michael Faraday (1791–1867) and James Clerk Maxwell (1831–1879).Karl-Heinz Schlote works as a historian of mathematics in the Arbeitsgruppe für Geschichte der Naturwissenschaften und Mathematik at the Sächsische Akademie der Wissenschaften in Leipzig, Germany. 相似文献
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Dr. Karl-Heinz Kiyek 《Mathematische Zeitschrift》1971,120(4):318-322
Ohne Zusammenfassung 相似文献
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