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11.
Kantak JB Bagade AV Mahajan SA Pawar SP Shouche YS Prabhune AA 《Applied biochemistry and biotechnology》2011,164(7):969-978
A lipolytic mesophilic fungus which produces lipase extracellularly was isolated from soil. Based on ITS1-5.8S–ITS4 region
sequences of ribosomal RNA, it was concluded that the isolate JK-1 belongs to genus Rhizopus and clades with Rhizopus oryzae. The present paper reports the screening, isolation, identification, and optimization of fermentation conditions for the
production of lipase (EC 3.1.1.3). Culture conditions were optimized, and the highest lipase production was observed in basal
medium with corn steep liquor as nitrogen source and glucose as carbon source. Maximum lipase production was observed at 72 h,
which is about 870 U/ml. Optimization of fermentation conditions resulted in 16-fold enhancement in enzyme production. 相似文献
12.
We have deposited relatively thick (∼60 nm) Ge layers on Br-passivated Si(111) substrates by thermal evaporation under high
vacuum conditions at room temperature. Ge has grown in a layer-plus-island mode although it is different from the Stranski-Krastanov
growth mode observed in epitaxial growth. Both the islands and the layer are nanocrystalline. This appears to be a consequence
of reduction of surface free energy of the Si(111) substrate by Br-passivation. The size distribution of the Ge nanoislands
has been determined. The Br-Si(111) substrates were prepared by a liquid treatment, which may not produce exactly reproducible
surfaces. Nevertheless, some basic features of the nanostructural island growth are reasonably reproducible, while there are
variations in the details of the island size distribution. 相似文献
13.
JB Pendry 《Contemporary Physics》2013,54(3):191-202
Light bends the wrong way in materials where both ε and μ are negative as was pointed out in 1968, but the absence of natural materials with this property led to neglect of the subject until 1999 when it was shown how to make artificial materials, metamaterials, with negative μ. The rapid advance of the subject since that date, both in theory and experiment, is reflected in the exponential growth of publications now at the 200 per year level and still growing. This interest is explained by the sudden availability of a qualitatively different class of electromagnetic materials combined with the quite startling properties which these materials appear to have; all of which provokes debate as each new facet of their behaviour is revealed. Experiment has been vital to resolution of controversy and has chiefly been in the microwave region of the spectrum though there is potential in the optical region currently being explored by several groups. 相似文献
14.
An extracellular lipase-producing fungus was isolated from oil-rich soil. This fungus belongs to the genus Rhizopus and clades with Rhizopus oryzae. Lipase was purified to homogeneity from this novel fungal source using ammonium sulphate precipitation followed by Q-Sepharose
chromatography. The extracellular lipase was purified 8.6–fold, and enzymatic properties were studied. The molecular mass
of the purified enzyme was estimated to be 17 kD by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and 16.25 kD
by matrix-assisted laser desorption ionization/time-of-flight analysis. The native molecular mass was estimated to be 17.5
kD by gel filtration, indicating the protein to be monomer. The optimum pH and temperature for the enzyme catalysis were 7.0 °C
and 40 °C, respectively. Enzyme was stable in pH range 6.0–7.0 and retains 95–100% activity when incubated at 50 °C for 1 h.
The pI of the purified lipase was 4.2. Enzyme was stable in the organic solvents such as ethanol, hexane and methanol for
2 h. Purified enzyme was used for transesterification of oleic acid in the presence of ethanol for production of oleic acid
ethyl ester with a conversion efficiency of 66% after 24 h at 30 °C. 相似文献
15.
Previously reported theories for cyclical electrical field flow fractionation (CyElFFF) are severely limited in that they do not account for diffusion, steric, or electric double layer effects. Experiments have shown that these theories overpredict the retention of particles in CyElFFF. In this work, we present a model for prediction of steric, diffusion, and electrical effects. The electrical double layer effects are treated using a lumped electrical circuit model that accounts for the field shielding by the electrical double layer formed at the electrode-carrier interface. The electrical effects are shown to dominate retention times and outweigh the contributions of diffusion and particle size. Detailed results from the simulations are presented in this work, and a comparison between the theoretical and experimental results obtained from the retentions of polystyrene particle standards is presented in this paper. The models are shown to correctly predict the retention of the polystyrene standards in CyElFFF with a reasonable error, while existing models are shown to have significant failings. 相似文献
16.
G Viesti M Lunardon D Fabris G Nebbia M Cinausero E Fioretto G Prete JB Natowitz K Hagel R Wada 《Pramana》1999,53(3):395-404
The transition of the level density parameter a
off from the low excitation energy value a
off=A/8 MeV−1 to the Fermi gas value a
FG ∼ A/15 MeV−1 was discovered a few years ago studying particle spectra evaporated from hot compound systems of A∼ 160. A number of experiments have been recently performed to confirm the earlier findings and extend the investigation to
other mass regions and to higher excitation energies. Furthermore, precision coincidence experiments have been done in the
lead region in which evaporation residues are tagged by low energy gammarays. Those experiments open the possibility of a
detailed study of the level densities in nuclei where the shell effects are important. 相似文献
17.
A new and efficient scheme for the synthesis of one of the best known inhibitors of botulinum neurotoxin serotype A (BoNTA) is reported herein. The synthetic route involves two palladium-catalyzed C-H functionalization reactions, formally activating three C-H bonds. 相似文献
18.
Metal-free intermolecular oxidative C-N bond formation via tandem C-H and N-H bond functionalization
Kantak AA Potavathri S Barham RA Romano KM DeBoef B 《Journal of the American Chemical Society》2011,133(49):19960-19965
The development of a novel intermolecular oxidative amination reaction, a synthetic transformation that involves the simultaneous functionalization of both a N-H and C-H bond, is described. The process, which is mediated by an I(III) oxidant and contains no metal catalysts, provides a rapid and green method for synthesizing protected anilines from simple arenes and phthalimide. Mechanistic investigations indicate that the reaction proceeds via nucleophilic attack of the phthalimide on an aromatic radical cation, as opposed to the electrophilic aromatic amination that has been reported for other I(III) amination reactions. The application of this new reaction to the synthesis of a variety of substituted aniline derivatives is demonstrated. 相似文献