Myoglobin Detection: Utilizing Functional Magnetic Hybrid and Silica Micro Particles and Simple Molding Based Micro Channel Fabrication

A magnetic separator attached in a zig zag flow channel assembly has been fabricated to detect myoglobin on the micro particles. An anti‐myoglobin conjugated magnetic hybrid and myoglobin conjugated silica micro particles were prepared and used for myoglobin detection on channel. For the detections, the competitive assay principle was followed based on affinity interaction of anti‐myoglobin‐myoglobin. The caliberation curve was plotted based on the deposit percentage of myoglobin conjugated silica micro particles. The calibration curve of the myoglobin showed the linear range between 1 × 10^?8 ‐ 5 × 10^?11 (M) (R² = 0.9944). The minimum detectable concentration was 300 pg/mL. Hence all that concludes that, the application of zig zag flow channel with magnetic separator has offered new, simple and rapid approach for detecting myoglobin in whole blood samples with in 30 min.     

New phenyl derivatives from endophytic fungus Botryosphaeria sp. SCSIO KcF6 derived of mangrove plant Kandelia candel

Two new phenyl derivatives (1 and 3), along with two new natural products (4 and 5), and three known compounds (2, 6 and 7), were isolated from an endophytic fungus Botryosphaeria sp. SCSIO KcF6. The structures of these compounds 1–7 were elucidated by the extensive 1D and 2D-NMR and HRESIMS Data analysis, and compared with those of reported data. The absolute configuration of the compounds 1 and 3 were assigned by optical rotation and CD data. The isolated compounds were evaluated for their cytotoxic, anti-inflammatory (COX-2) and antimicrobial activities. Compound 3 exhibited a specific COX-2 inhibitory activity with the IC₅₀ value of 1.12 μM.     

Charge‐assisted hydrogen‐bonded supramolecular networks in acetoguanaminium hydrogen phthalate,acetoguanaminium hydrogen maleate and acetoguanaminium 3‐hydroxypicolinate monohydrate

In 2,4‐diamino‐6‐methyl‐1,3,5‐triazin‐1‐ium (acetoguanaminium) hydrogen phthalate, C₄H₈N₅⁺·C₈H₅O₄⁻, (I), acetoguanaminium hydrogen maleate, C₄H₈N₅⁺·C₄H₃O₄⁻, (II), and acetoguanaminium 3‐hydroxypicolinate monohydrate, C₄H₈N₅⁺·C₆H₄NO₃⁻·H₂O, (III), the acetoguanaminium cations interact with the carboxylate groups of the corresponding anions via a pair of nearly parallel N—H...O hydrogen bonds, forming R₂²(8) ring motifs. In (II) and (III), N—H...N base‐pairing is observed, while there is none in (I). In (II), a series of fused R₃²(8), R₂²(8) and R₃²(8) hydrogen‐bonded rings plus fused R₂²(8), R₆²(12) and R₂²(8) ring motifs occur alternately, aggregating into a supramolecular ladder‐like arrangement. In (III), R₂²(8) motifs occur on either side of a further ring formed by pairs of N—H...O hydrogen bonds, forming an array of three fused hydrogen‐bonded rings. In (I) and (II), the anions form a typical intramolecular O—H...O hydrogen bond with graph set S(7), whereas in (III) an intramolecular hydrogen bond with graph set S(6) is formed.     

Fabrication of 105Y,Alpha 1‐Anti Trypsin Peptide Conjugated Hybrid Nanoparticles for Biological Applications

In this report, we describe the characterizations and applications of hybrid nanoparticles. These nanoparticles have been synthesized by combination of organometallic, polymerization process and functionalized with a specific peptide for targeting expressed serpin‐enzyme complex (SEC) receptor of human hepatoma HepG2 cells. By using peptide conjugated hybrid nanoparticles, the specific receptor targeting, collections of cells were successfully achieved. The cell collection results indicated that, the maximum up to 95.32% of HepG2 cell were collected. The 5‐dimethylthiazol‐2‐yl‐2,5‐diphenyltetrazolium bromide (MTT) assay of HepG2 cells incubated with these nanoparticles indicated that, the peptide conjugated hybrid nanoparticles did not possess significant cytotoxicity. The rotating magnetic field induced cell death studies indicated that, the HepG2 cell showed up to 70% of cell death was induced by hybrid nanoparticles under magnetic field. Concluding, these studies demonstrate that the hybrid nanoparticles have the capability of effective separation, imaging, targeting and killing of the human hepatoma cells.     

Stereoselective Synthesis of Polyfunctionalized Nitrothianes: Enhancement of Stereoselectivity by Microwaves

The Michael addition of nitromethane to (Z,Z)-2,2′-thiobis(1,3-diarylprop-2-en-1-ones) in the presence of NaOEt in dimethylformamide (DMF)/alcohol under thermal conditions affords a diastereomeric mixture of 2a,6e-diaroyl-3a,5e-diaryl-4e-nitrothianes and 2e,6e-diaroyl-3e,5e-diaryl-4e-nitrothianes with a dr of ～3:1/4:1 respectively. This reaction under microwave irradiation in DMF/alcohol afforded solely 2a,6e-diaroyl-3a,5e-diaryl-4e-nitrothianes, disclosing enhancement of stereoselectivity by microwaves.

Supplemental materials are available for this article. Go to the publisher's online edition of Synthetic Communications® to view the free supplemental file.     

Identification and determination of naturally occurring folates in grains of rice (Oryza sativa L.) by UPLC-MS/MS analysis

The genetic potential and biofortification of India-grown rice with bioavailable folate has not been studied yet. The objectives of this study were to determine the folates concentration in four cultivars of rice through UPLC–MS/MS. Total folate concentration in rice cultivars ranged from 11.0 to 51 μg/100 g with a mean of 26.0 μg/100 g. Among the four rice cultivars, the pigmented grain cultivar Nootripathu possesses two-fold rich sources of total folates than the other three non-pigmented grain cultivars. The average value of 100 g serving of rice grains could provide the amount of recommended daily allowance (% RDA) of dietary folates (6.5%) for adults, which ranged from 2.7–12.7%. Among the 5 individual forms of folates, 5-methyltetrahydrofolate was most abundant in rice cultivars followed by 10-Formylfolic acid and folic acid. The result of this study has been useful for biofortification of folates in rice.     

Profiling bioactive flavonoids and carotenoids in select south Indian spices and nuts

Abstract

The objective of this study was to examine the bioactive flavonoids and carotenoids concentration in fifteen south Indian spice and two tree nut species using high performance liquid chromatography (HPLC). Among four flavonoids, catechin concentration was the highest in all spices and nuts and ranged between 97.1 and 1745.4 µg g^?1. Quercetin concentration was the greatest in cinnamon, followed by garlic and cumin and ranged from 0.4 to 65 µg g^?1 in other spices and nuts. Lutein concentration ranged from 0.1 to 102.8 µg g^?1. Of the spices and nuts studied, β-carotene concentration was highest in coriander leaves (74.7 µg g^?1), followed by red pepper (12.5 µg g^?1) and curry leaves (8.5 µg g^?1). This research shows that consumption of south Indian spices and nuts could substantially benefit consumers living in regions experiencing Vitamin A and other micronutrient deficiencies.     

Structural,spectroscopic properties and theoretical studies of (E)-1-(4-Bromophenyl)-3-(2,3,4-trimethoxyphenyl)prop-2-en-1-one as a potential anti-oxidant agent

The new chalcone compound namely (E)-1-(4-Bromophenyl)-3-(2,3,4-trimethoxyphenyl)prop-2-en-1-one (C₁₈H₁₇BrO₄) is crystallized in the monoclinic crystal system of P2₁/c space group. The unit cell dimensions are: a = 8.0753 (10) Å, b = 21.873 (3) Å, c = 9.3362 (12) Å, α = 90°, β = 99.369 (2), γ = 90° and Z = 4. The single crystal was grown using slow evaporation solution growth technique. The newly synthesized compound was characterized by using IR, ¹H- and ¹³C-NMR, UV-Vis and single crystal X-ray diffraction analyses. Quantum chemical method of Density Functional Theory (DFT) with B3LYP/6-311++G(d,p) has been employed to study the structural and spectral properties of the compound. The electronic absorption spectrum was calculated using the time dependent functional theory (TDDFT) method. The most stable conformer of the title chalcone is identified from the computational results. Hirshfeld surface analysis with fingerprint plots has been used as a graphical tool for visualization and understanding of intermolecular interactions. Intermolecular C?H····O interaction observed stabilize the crystal structure, forming an infinite one dimensional column. The effect of this intermolecular interaction in the solid state can be seen in the difference between the experimental and the theoretically optimized geometrical parameters. The crystal is transparent in the entire visible region and absorptive in the UV region. Information about the size, shape, charge density distribution and site of chemical reactivity of the molecule has been obtained by mapping electron potential (MEP). The other molecular properties like charge transfer are explain using Mulliken population analysis. The antioxidant test indicated that the 2,3,4-trimethoxy substitution on the bromide chalcone to be one of the favorable modification to enhance its metal chelating activity.     

Co-crystal structures of 2,4-diamino-6-phenyl-1,3,5-triazine – 4-chlorobenzoic acid/4-methoxybenzoic acid: Synthesis,structural and Hirshfeld surface analysis

The co-crystal structure of 2,4-diamino-6-phenyl-1,3,5-triazine with 4-chlorobenzoic acid/4-methoxybenzoic acid molecules are explain the link between the dimension and shape of their hydrogen-bonded assembly. The co-crystal structure of 2,4-diamino-6-phenyl-1,3,5-triazine with 4-chlorobenzoic acid/4-methoxybenzoic acid forming a cyclic R₂²(8) ring motif via N–H?O and O–H?N hydrogen bonding interactions, to form a supramolecular heterosynthon. In both the co-crystal structures, self-association of 2,4-diamino-6-phenyl-1,3,5-triazine moieties are connected via N-H^….N base pairs with cyclic R₂²(8) motif form a supramolecular homosynthon. In co-crystal II, the centrosymmetrically paired methoxy group of 4-methoxy benzoic acid form a supramolecular homosynthon via weak intermolecular C-H^….O hydrogen bonds, generating R₂²(6) ring motif. Both the co-crystal structures are stabilized by weak aromatic π-π and C—Cl???π stacking interactions. Hirshfeld surface and fingerprint plots were used to study the intermolecular interactions both of the crystal structures.