全文获取类型
收费全文 | 592篇 |
免费 | 3篇 |
国内免费 | 1篇 |
专业分类
化学 | 274篇 |
力学 | 6篇 |
数学 | 47篇 |
物理学 | 269篇 |
出版年
2021年 | 6篇 |
2019年 | 5篇 |
2017年 | 5篇 |
2016年 | 9篇 |
2014年 | 7篇 |
2013年 | 19篇 |
2012年 | 17篇 |
2011年 | 25篇 |
2010年 | 11篇 |
2009年 | 12篇 |
2008年 | 23篇 |
2007年 | 18篇 |
2006年 | 9篇 |
2005年 | 19篇 |
2004年 | 20篇 |
2003年 | 7篇 |
2002年 | 17篇 |
2001年 | 20篇 |
2000年 | 16篇 |
1999年 | 10篇 |
1996年 | 15篇 |
1995年 | 11篇 |
1994年 | 5篇 |
1993年 | 15篇 |
1992年 | 9篇 |
1991年 | 14篇 |
1990年 | 16篇 |
1989年 | 20篇 |
1988年 | 8篇 |
1987年 | 6篇 |
1986年 | 12篇 |
1985年 | 8篇 |
1984年 | 12篇 |
1983年 | 7篇 |
1982年 | 5篇 |
1981年 | 6篇 |
1980年 | 7篇 |
1979年 | 14篇 |
1978年 | 13篇 |
1977年 | 7篇 |
1976年 | 6篇 |
1975年 | 7篇 |
1973年 | 6篇 |
1972年 | 12篇 |
1971年 | 9篇 |
1970年 | 5篇 |
1969年 | 4篇 |
1968年 | 7篇 |
1967年 | 9篇 |
1966年 | 5篇 |
排序方式: 共有596条查询结果,搜索用时 15 毫秒
31.
Kagan Kerman Yasutaka Morita Yuzuru Takamura Mehmet Ozsoz Eiichi Tamiya 《Analytica chimica acta》2004,510(2):169-174
The unique binding event between Escherichia coli single-stranded DNA binding protein (SSB) and single-stranded oligonucleotides conjugated to gold (Au) nanoparticles is utilized for the electrochemical detection of DNA hybridization. SSB was attached onto a self-assembled monolayer (SAM) of single-stranded oligonucleotide modified Au nanoparticle, and the resulting Au-tagged SSB was used as the hybridization label. Changes in the Au oxidation signal was monitored upon binding of Au tagged SSB to probe and hybrid on the electrode surface. The amplified oxidation signal of Au nanoparticles provided a detection limit of 2.17 pM target DNA, which can be applied to genetic diagnosis applications. This work presented here has important implications with regard to combining a biological binding event between a protein and DNA with a solid transducer and metal nanoparticles. 相似文献
32.
Alam MS Kim IJ Nemati B O'Neill JJ Romero V Severini H Sun CR Wang P Zoeller MM Crawford G Fulton R Gan KK Kagan H Kass R Lee J Malchow R Morrow F Sung M White C Whitmore J Wilson P Butler F Fu X Kalbfleisch G Lambrecht M Skubic P Snow J Bortoletto D Brown DN Dominick J McIlwain RL Miao T Miller DH Modesitt M Schaffner SF Shibata EI Shipsey IP Battle M Ernst J Kroha H Roberts S Sparks K Thorndike EH Wang C Artuso M Goldberg M Haupt T Horwitz N Kennett R Moneti GC Playfer S Rozen Y Rubin P 《Physical review D: Particles and fields》1992,46(11):4822-4827
33.
Fulton R Jensen T Johnson DR Kagan H Kass R Morrow F Whitmore J Wilson P Bortoletto D Chen W Dominick J McIlwain RL Miller DH Ng CR Schaffner SF Shibata EI Shipsey IP Yao W Battle M Sparks K Thorndike EH Wang C Alam MS Kim IJ Li WC Romero V Sun CR Wang P Zoeller MM Goldberg M Haupt T Horwitz N Jain V Mestayer MD Moneti GC Rozen Y Rubin P Sharma V Skwarnicki T Thulasidas M Zhu G Csorna SE Letson T Alexander J Artuso M Bebek C Berkelman K Browder T Cassel DG Cheu E Coffman DM Crawford G DeWire JW 《Physical review D: Particles and fields》1991,43(3):651-663
34.
Kubota Y Nelson JK Perticone D Poling R Schrenk S Crawford G Fulton R Jensen T Johnson DR Kagan H Kass R Malchow R Morrow F Whitmore J Wilson P Bortoletto D Brown D Dominick J McIlwain RL Miller DH Modesitt M Ng CR Schaffner SF Shibata EI Shipsey IP Battle M Kroha H Sparks K Thorndike EH Wang C Alam MS Kim IJ Li WC Nemati B Romero V Sun CR Wang P Zoeller MM Goldberg M Haupt T Horwitz N Jain V Mestayer MD Moneti GC Rozen Y Rubin P Skwarnicki T Stone S Thusalidas M Yao W Zhu G Barnes AV Bartelt J 《Physical review D: Particles and fields》1991,44(3):593-600
35.
Kashparova V. P. Kagan E. Sh. Kashparov I. S. Zhukova I. Yu. 《Russian Journal of Applied Chemistry》2002,75(4):667-668
Diazotization of 4-amino-2,2,6,6-tetramethylpiperidine in acetic or sulfuric acid affords 2,2,6,6- tetramethyl-1,2,3,6-tetrahydropyridine in high yield. Under the same conditions, the corresponding nitroxyl radical transforms into 4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl. 相似文献
36.
Anthrone has been found to react with N-methyl maleimide in the presence of catalytic amounts of various chiral β-amino alcohols. The optically active cycloadduct 3a has been obtained in excellent yield. Several features of the reaction have been studied. 相似文献
37.
38.
39.
40.
Yae Eun Chong Manting Chiang Kiran Deshpande Simon Haroutounian Leonid Kagan Jong Bong Lee 《Biomedical chromatography : BMC》2019,33(11)
Ondansetron, a widely used antiemetic agent, is a P‐glycoprotein (P‐gp) substrate and therefore expression of P‐gp at the blood–brain barrier limits its distribution to the central nervous system (CNS), which was observed to be reversed by coadministration with P‐gp inhibitors. Tariquidar is a potent and selective third‐generation P‐gp inhibitor, and coadministration with ondansetron has shown improved ondansetron distribution to the CNS. There is currently no reported bioanalytical method for simultaneously quantifying ondansetron with a third‐generation P‐gp inhibitor. Therefore, we aimed to develop and validate a method for ondansetron and tariquidar in rat and human plasma samples. A full validation was performed for both ondansetron and tariquidar, and sample stability was tested under various storage conditions. To demonstrate its utility, the method was applied to a preclinical pharmacokinetic study following coadministration of ondansetron and tariquidar in rats. The presented method will be valuable in pharmacokinetic studies of ondansetron and tariquidar in which simultaneous determination may be required. In addition, this is the first report of a bioanalytical method validated for quantification of tariquidar in plasma samples. 相似文献