基于DNA损伤的蛋白调控网络研究

细胞生长的每个阶段都离不开蛋白质相互作用.研究细胞周期的功能、调控机理及参与调控的蛋白质之间的关系对生物工程等领域有重大的应用价值.本文通过研究电离辐射下生物体细胞的DNA损伤后,细胞内以p53为核心的扩展蛋白调控网络的功能、原理及其自修复机理,在现有蛋白网络基础上引入更多蛋白网络调控因子来建立蛋白调控网络,仿真模拟更为全面的细胞周期进程;并且从复杂网络图论和细胞周期调控两个方面分析扩展PMP调控网络的抗扰能力及自修复机理,结果表明:1)蛋白网络在对抗环境中出现的小扰动时具有较强的稳定性.但在面对蓄意攻击时网络的稳定性较差.2)受损的DNA能否被修复取决于p53蛋白的动力学行为,即低损伤与中损伤情况下,p53可诱导细胞周期进程阻滞来完成细胞的自修复;而当高损伤或过损伤时,p53蛋白浓度表现为周期振荡行为并诱导细胞凋亡.     

一种基于抖动和混沌技术的数字图像篡改检测及修复算法

本文提出了一种基于抖动和混沌技术的数字图像篡改检测及修复算法.该算法使用小波变换后的低频子图和抖动技术生成图像的认证及修复信息,在有效减少水印数据量的同时,将水印嵌入小波变换后的高频子图,从而达到水印的不可见性.运用混沌技术完成水印的嵌入和加密,并结合中国余数定理,进一步减少水印嵌入对图像质量的影响.实验证明,该算法兼顾了水印的不可见性和鲁棒性,并且能够一定程度修复篡改图像,在图像认证和修复方面具有较高的实用意义.     

含螺旋单元频率选择表面的宽频带强吸收复合吸波体

设计和制备了含螺旋单元频率选择表面吸波片的三层复合吸波体,上层和下层均为磁性吸波片,中间层为带缺口的螺旋单元频率选择表面.复合吸波体在总厚度分别为1.4,1.7和2.0 mm时,其反射率在-10d B以下的频带宽度分别达到了9.29,6.69和7.11 GHz,与不含有频率选择表面的吸波体相比较(其他参数相同),-10d B以下反射率带宽分别提高了159.5%,69.3%和129.4%,复合吸波体在总厚度低于吸波体时,也取得了更好的反射效果.带缺口圆螺旋单元的频率选择表面嵌入吸波体中,引入了额外的吸收频带,拓宽了吸波体的反射率频带宽度.仿真分析表明嵌入频率选择表面能够改善吸波体的阻抗匹配性,进而影响其反射率.     

Purification of the recombinant hepatitis B virus core antigen (rHBcAg) produced in the yeast Saccharomyces cerevisiae and comparative observation of its particles by transmission electron microscopy (TEM) and atomic force microscopy (AFM)

Hepatitis B virus core antigen (HBcAg) gene (C gene) was expressed in Saccharomyces cerevisiae and the products (rHBcAg or core particles) were purified from a crude lysate of the yeast by three steps: Sephrose CL-4B chromatography, Sucrose step-gradient ultracentrifugation and CsCl-isopycnic ultracentrifugation. It has been observed that HBcAg was synthesized in yeast cells as a particle consisting of polypeptides with a molecular weight of 21.5 kDa (p21.5). Results of ELISA test and density analysis of CsCl-isopycnic ultracentrifugation indicated that the purified products (rHBcAg particles) with HBcAg antigenicity mainly located at the densities of 1.27 and 1.40 g ml(-1), respectively. Observation and analysis of the purified rHBcAg products by TEM indicated that rHBcAg peptides could mainly self-assemble into two size classes of core particles. The larger particles were approximately 30.1 nm and the smaller were approximately 21.5 nm in mean diameter. Further observation and analysis of the same rHBcAg (core) particles by AFM also indicated that rHBcAg (core) particles were similar to the native HBcAg (core) particles from infected human hepatocytes and mainly composed of two size classes of partides core. The larger particles were approximately 31.3 nm and the smaller were approximately 22.5 nm in mean diameter which was similar to the results obtained by TEM. All results from both TEM and AFM suggested that core particles (capsids) produced in S. cerevisiae possessed dimorphism.     

Information flow between stock markets: A Koopman decomposition approach

Stock markets in the world are linked by complicated and dynamical relationships into a temporal network.Extensive works have provided us with rich findings from the topological properties and their evolutionary trajectories,but the underlying dynamical mechanism is still not in order.In the present work,we proposed a technical scheme to reveal the dynamical law from the temporal network.The index records for the global stock markets form a multivariate time series.One separates the series into segments and calculates the information flows between the markets,resulting in a temporal market network representing the state and its evolution.Then the technique of the Koopman decomposition operator is adopted to find the law stored in the information flows.The results show that the stock market system has a high flexibility,i.e.,it jumps easily between different states.The information flows mainly from high to low volatility stock markets.And the dynamical process of information flow is composed of many dynamic modes distribute homogenously in a wide range of periods from one month to several ten years,but there exist only nine modes dominating the macroscopic patterns.     

Investigations on the heat transfer performance of edge-shaped finned-tubes

The third generation enhanced heat transfer technologies, such as three-dimensional fin and dimple, are still important means of improving energy efficiency and will continue to be challenging issues. This paper concentrates on the analysis of the condensation heat transfer performance of an edge-shaped finned-tube fabricated by extrusion–ploughing process. Experimental results show that the overall heat transfer coefficient increases with increases of volumetric flow rate of cold water and heat flux whereas the shell side heat transfer coefficient decreases with volumetric flow rate and heat flux increasing. At the similar volumetric flow rate, the shell side heat transfer coefficient of the edge-shaped finned-tube is 4–6 times larger than that of the smooth tube. At the similar volumetric flow rate, the shell side heat transfer coefficient of edge-shaped finned-tube increases with ploughing depth increasing. At the same temperature difference between wall and vapor, the shell side heat transfer coefficient is also higher than what had been reported in the literature.     

泡沫铝率相关性能的有限元模拟

构建了三维随机分布球形泡孔模型,模拟开、闭孔混合结构泡沫铝材料的微细观结构,并通过有限元方法计算了10~104 s-1应变率范围内、孔隙率35%~65%泡沫铝材料的率相关性以及应变率和相对密度变化对泡沫铝动态压缩力学性能的影响。研究表明:中、低应变率下,泡沫铝材料率相关性能主要取决于基体材料的应变率敏感性;高应变率下,泡沫铝材料率相关性能受基体材料的应变率敏感性以及微结构惯性联合作用,且相对密度较低泡沫铝材料的微结构惯性效应更显著。     

Graft polymerization of 2-hydroxyethyl methacrylate via ATRP with poly(acrylonitrile-co-p-chloromethyl styrene) as a macroinitiator

Amphiphilic graft copolymers are excellent additives for the development of antifouling membranes by nonsolvent induced phase separation. We report a convenient approach to the synthesis of novel graft copolymers with hydrophobic polyacrylonitrile (PAN) backbones and hydrophilic poly(2-hydroxyethyl methacrylate) (PHEMA) side chains. Atom transfer radical polymerization (ATRP) of 2-hydroxyethyl methacrylate was carried out with poly(acrylonitrile-co-p-chloromethyl styrene) (PAN-co-PCMS) as a macroinitiator in the presence of CuCl/2,2’-bipyridine at 50 °C in dimethyl sulfoxide. Kinetics of the graft polymerization was also evaluated. The synthesis of poly(acrylonitrile-co-p-chloromethyl styrene-g-2-hydroxyethyl methacrylate) (PAN-co-(PCMS-g-PHEMA)) can be relatively controlled when CMS (the ATRP sites) unit in the macroinitiator is around 5 mol%. Both the macroinitiators and graft copolymers were characterized by FTIR, NMR and GPC. The surface morphology and wettability of the copolymer films were studied by AFM and water contact angle measurement, respectively. We demonstrate that phase segregation between the PAN-co-PCMS backbones and the PHEMA side chains takes place and the surface hydrophilicity of the graft copolymers increases with the length of the PHEMA side chains. Because these amphiphilic graft copolymers can be synthesized in mass, they will be useful as latent additives for the fabrication of advanced PAN separation membranes.     

Synthesis and biological evaluation of lycorine derivatives as dual inhibitors of human acetylcholinesterase and butyrylcholinesterase

ABSTRACT: BACKGROUND: Alzheimer's disease (AD) is a neurologically degenerative disorder that affects more than 20 million people worldwide. The selective butyrylcholinesterase (BChE) inhibitors and bivalent cholinesterase (ChE) inhibitors represent new treatments for AD. FINDINGS: A series of lycorine derivatives (1--10) were synthesized and evaluated for anti-cholinesterase activity. Result showed that the novel compound 2-O-tert-butyldimethylsilyl-1-O-(methylthio)methyllycorine (7) was a dual inhibitor of human acetylcholinesterase (hAChE) and butyrylcholinesterase (hBChE) with IC50 values of 11.40 [PLUS-MINUS SIGN] 0.66 muM and 4.17 [PLUS-MINUS SIGN] 0.29 muM, respectively. The structure-activity relationships indicated that (i) the 1-O-(methylthio)methyl substituent in lycorine was better than the 1-O-acetyl group for the inhibition of cholinesterase; (ii) the acylated or etherified derivatives of lycorine and lycorin-2-one were more potent against hBChE than hAChE; and (iii) the oxidation of lycorine at C-2 decreases the activity. CONCLUSION: Acylated or etherified derivatives of lycorine are potential dual inhibitors of hBChE and hAChE. Hence, further study on the modification of lycorine for ChE inhibition is necessary.     

Alcohol‐Processable Organic Amorphous Electrolytes as an Effective Electron‐Injection Layer for Organic Light‐Emitting Diodes

A new series of monoammonium‐based organic electrolytes with the tetrafluoroborate (BF₄^?) counteranion have been synthesized. Replacing the pendant ethyl groups in the fluorenyl unit with 4‐ethoxyphenyl groups dramatically improves both solubility and morphological stability. The characterization of the alcohol‐processable amorphous ionic compounds as an electron‐injection layer in organic light‐emitting diodes (OLEDs) reveals that the organic electrolyte that comprises a rigid linear‐conjugated unit provides better device performance, with respect to its counterpart containing a branched bulky moiety. The capability of these compounds to facilitate electron injection from air‐stable aluminum metal is preliminarily discussed on the basis of the investigations of the electron‐only devices and photovoltaic experiments.