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961.
962.
Light-triggered molecule-scale drug dosing devices 总被引:1,自引:0,他引:1
McCoy CP Rooney C Edwards CR Jones DS Gorman SP 《Journal of the American Chemical Society》2007,129(31):9572-9573
963.
Benkoski JJ Bowles SE Korth BD Jones RL Douglas JF Karim A Pyun J 《Journal of the American Chemical Society》2007,129(19):6291-6297
The assembly and direct imaging of ferromagnetic nanoparticles into one-dimensional mesostructures (1-D) are reported. Polymer-coated ferromagnetic colloids (19 nm, 24 nm) were assembled at a crosslinkable oil-water interface under both magnetic field induced and zero-field conditions and permanently fixed into 1-D mesoscopic polymer chains (1-9 mum) in a process referred to as Fossilized Liquid Assembly (FLA). In the FLA process, nanoparticle chains were fixed at the oil interface through photopolymerization, enabling direct visualization of organized mesostructures using atomic force microscopy. Using the FLA methodology, we systematically investigated different conditions and demonstrated that dispersed ferromagnetic colloids possess sufficient dipolar interactions to organize into mesoscopic assemblies. Application of an external magnetic field during assembly enabled the formation of micron-sized chains which were aligned in the direction of the applied field. This universal methodology is an attractive alternative technique to cryogenic transmission electron microscopy (cryo-TEM) for the visualization of nanoparticle assembly in dispersed organic media. 相似文献
964.
Lenz T Bonnist EY Pljevaljcić G Neely RK Dryden DT Scheidig AJ Jones AC Weinhold E 《Journal of the American Chemical Society》2007,129(19):6240-6248
We report the crystal structure of the DNA adenine-N6 methyltransferase, M.TaqI, complexed with DNA, showing the fluorescent adenine analog, 2-aminopurine, flipped out of the DNA helix and occupying virtually the same position in the active site as the natural target adenine. Time-resolved fluorescence spectroscopy of the crystalline complex faithfully reports this state: base flipping is accompanied by the loss of the very short ( approximately 50 ps) lifetime component associated with fully base-stacked 2-aminopurine in DNA, and 2-aminopurine is subject to considerable quenching by pi-stacking interactions with Tyr108 in the catalytic motif IV (NPPY). This proves 2-aminopurine to be an excellent probe for studying base flipping by M.TaqI and suggests similar quenching in the active sites of DNA and RNA adenine-N6 as well as DNA cytosine-N4 methyltransferases sharing the conserved motif IV. In solution, the same distinctive fluorescence response confirms complete destacking from DNA and is also observed when the proposed key residue for base flipping by M.TaqI, the target base partner thymine, is substituted by an abasic site analog. The corresponding cocrystal structure shows 2-aminopurine in the active site of M.TaqI, demonstrating that the partner thymine is not essential for base flipping. However, in this structure, a shift of the 3' neighbor of the target base into the vacancy left after base flipping is observed, apparently replicating a stabilizing role of the missing partner thymine. Time-resolved fluorescence and acrylamide quenching measurements of M.TaqI complexes in solution provide evidence for an alternative binding site for the extra-helical target base within M.TaqI and suggest that the partner thymine assists in delivering the target base into the active site. 相似文献
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966.
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968.
969.
Polyfluorenes without monoalkylfluorene defects 总被引:4,自引:0,他引:4
Cho SY Grimsdale AC Jones DJ Watkins SE Holmes AB 《Journal of the American Chemical Society》2007,129(39):11910-11911
970.
Transcellular diffusion across the absorptive epithelial cells (enterocytes) of the small intestine is the main route of absorption for most orally administered drugs. The process by which lipophilic compounds transverse the aqueous environment of the cytoplasm, however, remains poorly defined. In the present study, we have identified a structurally diverse group of lipophilic drugs that display low micromolar binding affinities for a cytosolic lipid-binding protein - intestinal fatty acid-binding protein (I-FABP). Binding to I-FABP significantly enhanced the transport of lipophilic drug molecules across a model membrane, and the degree of transport enhancement was related to both drug lipophilicity and I-FABP binding affinity. These data suggest that intracellular lipid-binding proteins such as I-FABP may enhance the membrane transport of lipophilic xenobiotics and facilitate drug access to the enterocyte cytoplasm and cytoplasmic organelles. 相似文献