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571.
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573.
Philipp Reissaus Tomas Waldemarsson Jürgen Blum Dominik Clément Isabel Llamas Harald Mutschke Frank Giovane 《Journal of nanoparticle research》2006,8(5):693-703
In order to find reliable collector surfaces for the Mesospheric Aerosol – Genesis, Interaction and Composition (MAGIC) sounding rocket experiment, intended to collect atmospheric nanoparticles, the sticking efficiency of nanoparticles was measured on several targets of different materials. The nanoparticles were generated by a molecular beam apparatus in Jena, Germany, by laser ablation (Al2O3 particles, diameter 5–50 nm) and by laser pyrolysis (carbon particles, diameter 10–20 nm). In a vacuum environment (>10−5 mbar) the particles condensed from the gas phase, formed a particle beam, and were accelerated to ∼
∼1 km/s. The sticking efficiency on the target materials carbon, gold and grease was measured by a microbalance. Results demonstrate moderate to high sticking probabilities. Thus, the capture and retrieval of atmospheric nanoparticles was found to be quantitatively feasible. 相似文献
574.
Faina Gelman Jochanan Blum Herbert Schumann David Avnir 《Journal of Sol-Gel Science and Technology》2003,26(1-3):43-46
Silica sol-gel entrapped acids, bases and organometallic catalysts are used successfully in one-pot reactions without interfering with each other. Both physically and covalently entrapped acids and bases were employed in these processes, including polystyrene sulfonic acid, 1-propane sulfonic acid, molybdosilicic acid (SiO2-MoO3), poly[(vinylbenzyltrimethyl)ammonium] hydroxide and 1,5,7-triazabicyclo[4.4.0]decene. The entrapped metal-based catalysts are Rh2Co2(CO)12 and RuCl2(PPh3)3. The one-pot reactions carried out with these heterogenized reagents and catalysts include formation of alkenes under acidic and basic conditions, C—C bond formation by condensation reactions with an -carbon to a carbonyl, hydroformylation and hydrogenation. 相似文献
575.
Conditional protein splicing: a new tool to control protein structure and function in vitro and in vivo 总被引:9,自引:0,他引:9
Mootz HD Blum ES Tyszkiewicz AB Muir TW 《Journal of the American Chemical Society》2003,125(35):10561-10569
Protein splicing is a naturally occurring process in which an intervening intein domain excises itself out of a precursor polypeptide in an autocatalytic fashion with concomitant linkage of the two flanking extein sequences by a native peptide bond. We have recently reported an engineered split VMA intein whose splicing activity in trans between two polypeptides can be triggered by the small molecule rapamycin. In this report, we show that this conditional protein splicing (CPS) system can be used in mammalian cells. Two model constructs harboring maltose-binding protein (MBP) and a His-tag as exteins were expressed from a constitutive promoter after transient transfection. The splicing product MBP-His was detected by Western blotting and immunoprecipitation in cells treated with rapamycin or a nontoxic analogue thereof. No background splicing in the absence of the small-molecule inducer was observed over a 24-h time course. Product formation could be detected within 10 min of addition of rapamycin, indicating the advantage of the posttranslational nature of CPS for quick responses. The level of protein splicing was dose dependent and could be competitively attenuated with the small molecule ascomycin. In related studies, the geometric flexibility of the CPS components was investigated with a series of purified proteins. The FKBP and FRB domains, which are dimerized by rapamycin and thereby induce the reconstitution of the split intein, were fused to the extein sequences of the split intein halves. CPS was still triggered by rapamycin when FKBP and FRB occupied one or both of the extein positions. This finding suggests yet further applications of CPS in the area of proteomics. In summary, CPS holds great promise to become a powerful new tool to control protein structure and function in vitro and in living cells. 相似文献
576.
J.Z.Bai O.Bardon I.Blum A. Breakstone T.Burnett G.Chen G.P.Chen H.F.Chen J.Chen S.M.Chen Y.Q.Chen Y.Chen Y.B.Chen B.S.Cheng R.F.Cowan X.Z.Cui H.L.Ding Z.Z.DU W.Dunwoodie X.L.Fan J.Fang M.Fero C.S.Gao M.L.Gao S.Q.Gao P.Gratton J.H.Gu S.D.Gu W.X.Gu Y.F.Gu 《中国物理 C》1996,(12)
利用北京谱仪在北京正负电子对撞机上采集的350万(2S)事例,通过(2S)→γπ-和γK+K-反应道测量了Xc0的总宽度.由MonteCarlo模拟给出的质量分辨函数,利用拟合Xc2谱形得到的质量分辨作标定后,用于Xc0宽度的拟合,得到Xc0的宽度为(15.0)MeV.同时定出了XcJ(J=0,2)到π+π=和K+K-的衰变分支比.结果为B(Xc0→π+π-)=(4.27±0.23±O.60)×10-3;B(Xc0→K+K-)=(3.44±0.21±0.47×10-3;B(Xc2→π+π-)=(1.52±0.17±0.29)×10-3和B(Xc2→K+K-)=(5.2±1.1±1.8)×10-4,其中第一项误差为统计误差,第二项为系统误差。 相似文献
577.
James Blum Mark Lammers Alexander M. Powell Özgür Yılmaz 《Journal of Fourier Analysis and Applications》2010,16(3):382-381
A new class of alternative dual frames is introduced in the setting of finite frames for ℝ
d
. These dual frames, called Sobolev duals, provide a high precision linear reconstruction procedure for Sigma-Delta (ΣΔ) quantization
of finite frames. The main result is summarized as follows: reconstruction with Sobolev duals enables stable rth order Sigma-Delta schemes to achieve deterministic approximation error of order O(N-r)\mathcal{O}(N^{-r}) for a wide class of finite frames of size N. This asymptotic order is generally not achievable with canonical dual frames. Moreover, Sobolev dual reconstruction leads
to minimal mean squared error under the classical white noise assumption. 相似文献
578.
Longitudinal magnetization of a57Co in iron metal foil source and an iron metal foil absorber in a uniform external magnetic field results in a simple three-line
magnetic hyperfine absorption spectrum. Measurement of the spectral splitting as a function of applied magnetic field yields
the57Fe excited-and ground-state,g-factors. 相似文献
579.
Hernán G. Abeledo Yosef Blum Uriel G. Rothblum 《International Journal of Game Theory》1996,25(2):161-176
This paper continues recent work that introduced algebraic methods for studying the stable marriage problem of Gale and Shapley [1962]. Vande Vate [1989] and Rothblum [1992] identified a set of linear inequalities which define a polytope whose extreme points correspond to the stable matchings. Points in this polytope are called fractional stable matchings. Here we identify a unique representation of fractional stable matchings as a convex combination of stable matchings that are arrangeable in a man-decreasing order. We refer to this representation and to a dual one, in terms of woman-decreasing order, as the canonical monotone representations. These representations can be interpreted as time-sharing stable matchings where particular stable matchings are used at each time-instance but the scheduled stable matchings are (occasionally) switched over time. The new representations allow us to extend, in a natural way, the lattice structure of the set of stable matchings to the set of all fractional stable matchings. 相似文献
580.
Marc‐Michael Blum 《Nachrichten aus der Chemie》2015,63(12):1200-1203