A thioester substrate binds to the enzyme Arthrobacter thioesterase in two ionization states: evidence from Raman difference spectroscopy

4‐Hydroxybenzoyl‐CoA (4‐HB‐CoA) thioesterase from Arthrobacter is the final enzyme catalyzing the hydrolysis of 4‐HB‐CoA to produce coenzyme A and 4‐hydroxybenzoic acid in the bacterial 4‐chlorobenzoate dehalogenation pathway. Using a mutation E73A that blocks catalysis, stable complexes of the enzyme and its substrate can be analyzed by Raman difference spectroscopy. Here we have used Raman difference spectroscopy, in the non‐resonance regime, to characterize 4‐HB‐CoA bound in the active site of the E73A thioesterase. In addition, we have characterized complexes of the wild‐type enzyme complexed with the unreactive substrate analog 4‐hydroxyphenacyl‐CoA (4‐HP‐CoA). Both sets of complexes show evidence for two forms of the ligand in the active site: one population has the 4‐hydroxy group protonated, 4‐OH; while the second has the group as the hydroxide, 4‐O⁻. For bound 4‐HP‐CoA, X‐ray data show that glutamate 78 is close to the 4‐OH in the complex and it is likely that this is the proton acceptor for the 4‐OH proton. Although the pK_a of the 4‐OH group on the free substrate in aqueous solution is 8.6, the relative populations of ionized and neutral 4‐HB‐CoA bound to E73A remain invariant between pH 7.3 and 9.8. The invariance with pH suggests that the 4‐OH and the ‐COO⁻ of E78 constitute a tightly coupled pair where their separate pK_a ‘s lose their individual qualities. Narrow band profiles are seen in the CO double bond and C‐S regions, suggesting that the hydrolyzable thioester group is rigidly bound in the active site in a syn gauche conformation. Copyright © 2011 John Wiley & Sons, Ltd.     

The first pulsar discovered by FAST

<正>To assist with the commissioning [1] of the Five-hundredmeter Aperture Spherical radio Telescope (FAST), we performed a pulsar search, with the primary goal of developing and testing the pulsar data acquisition and processing pipelines. We tested and used three pipelines, two (P1 and P2 hereafter) searched for the periodic signature of pulsars     

On proximality with Banach density one

Let (X,T)$(X,T)$ be a topological dynamical system. A pair of points (x,y)∈X²$(x,y)\in X^2$ is called Banach proximal if for any ε>0$\epsilon >0$, the set {n∈_Z+:d(Tⁿx,Tⁿy)<ε}$\{n\in {\mathbb{Z}}_{+}:d(T^{n}x,T^{n}y)<\epsilon \}$ has Banach density one. We study the structure of the Banach proximal relation. A useful tool is the notion of the support of a topological dynamical system. We show that a dynamical system is strongly proximal if and only if every pair in X²$X^2$ is Banach proximal. A subset S of X is Banach scrambled if every two distinct points in S form a Banach proximal pair but not asymptotic. We construct a dynamical system with the whole space being a Banach scrambled set. Even though the Banach proximal relation of the full shift is of first category, it has a dense Mycielski invariant Banach scrambled set. We also show that for an interval map it is Li–Yorke chaotic if and only if it has a Cantor Banach scrambled set.     

Radicals in skew polynomial and skew Laurent polynomial rings

We provide a general procedure for characterizing radical-like functions of skew polynomial and skew Laurent polynomial rings under grading hypotheses. In particular, we are able to completely characterize the Wedderburn and Levitzki radicals of skew polynomial and skew Laurent polynomial rings in terms of ideals in the coefficient ring. We also introduce the T-nilpotent radideals, and perform similar characterizations.     

一种高功率共面馈电脉冲辐射天线的设计实验

设计了一种高功率共面馈电脉冲辐射天线。该天线馈源采用双组电大尺寸共面极板馈臂,将馈电点置于焦点处,与直径2.1 m的抛物反射面相配合,实现超宽谱短脉冲的有效定向辐射。提出了一种由同轴输入到4端输出的高功率馈电巴伦,解决了高功率情况下同轴线到4馈臂共面馈电问题。对所设计天线进行测试实验,结果表明：在馈电脉冲宽度为450 ps、峰值电压为142 kV时,辐射因子达到800 kV。     

微带传输线束流信号展宽处理设计

对束流信号进行展宽处理是储存环逐束团位置测量RF前端设计的重要工作之一。针对上海光源储存环束流信号的特点,对束流信号展宽进行分析,采用低通滤波器和包络检波联合的方法对信号进行展宽。考虑到实现过程需要对信号进行ps量级的延时,使用微带传输线对该方法进行了具体设计。对微带传输线等效为集总参数LC元件进行推导以进行低通滤波器设计,给出了阻抗匹配方案以实现宽带功率分配器与合成器。仿真和实测结果表明,所设计的电路能够对束流信号进行有效展宽处理。     

New complexity analysis of a full-Newton step feasible interior-point algorithm for $$P_*(\kappa )$$-LCP

In this paper, we consider a full-Newton step feasible interior-point algorithm for \(P_*(\kappa )\)-linear complementarity problem. The perturbed complementarity equation \(xs=\mu e\) is transformed by using a strictly increasing function, i.e., replacing \(xs=\mu e\) by \(\psi (xs)=\psi (\mu e)\) with \(\psi (t)=\sqrt{t}\), and the proposed interior-point algorithm is based on that algebraic equivalent transformation. Furthermore, we establish the currently best known iteration bound for \(P_*(\kappa )\)-linear complementarity problem, namely, \(O((1+4\kappa )\sqrt{n}\log \frac{n}{\varepsilon })\), which almost coincides with the bound derived for linear optimization, except that the iteration bound in the \(P_{*}(\kappa )\)-linear complementarity problem case is multiplied with the factor \((1+4\kappa )\).     

Structure transition and photoluminescence of CdS/Si nanoheterostructure array on thermal annealing

CdS/Si heterojunctions have been prepared through growing CdS nanocrystallites (nc-CdS) on the silicon nanoporous pillar array (Si-NPA) by the chemical bath deposition method. Cadmium nanocrystallites (nc-Cd) have been observed and ascribed to the reducibility of Si-NPA. The reason for the appearance of CdO is indistinct and the related work will be done in the future. The blue, green and red emissions are ascribed to the silicon oxide layer, band gap of nc-CdS and the sulphur vacancies, respectively. Redshift and blueshift with the annealing temperature about green emissions are contributed to quantum size effect and the structure transition from nc-Cd to CdO. It is beneficial for investigating the structures and defects to the application of CdS/Si in the optoelectronic field.     

Symmetry Reductions,Group‐Invariant Solutions,and Conservation Laws of a (2+1)‐Dimensional Nonlinear Schrödinger Equation in a Heisenberg Ferromagnetic Spin Chain

Nonlinear spin excitations in ferromagnetic spin chains are studied for spintronic and magnetic devices including magnetic‐field sensors and for high‐density data storage. Here, (2+1)‐dimensional nonlinear Schrödinger equation is investigated, which describes the nonlinear spin dynamics for a Heisenberg ferromagnetic spin chain. Lie point symmetry generators and Lie symmetry groups of that equation are derived. Lie symmetry groups are related to the time, space, scale, rotation transformations, and Galilean boosts of that equation. Certain solutions, which are associated with the known solutions, are constructed. Based on the Lie symmetry generators, the reduced systems of such an equation are obtained. Based on the polynomial expansion and through one of the reduced systems, group‐invariant solutions are constructed. Soliton‐type group‐invariant solutions are graphically investigated and effects of the magnetic coupling coefficients, that is, α₁, α₂, α₃, and α₄, on the soliton's amplitude, width, and velocity are discussed. It is seen that α₁, α₂, α₃, and α₄ have no influence on the soliton's amplitude, but can affect the soliton's velocity and width. Lax pair and conservation laws of such an equation are derived.