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M. Jonathan Fray Paul Allen Paul R. Bradley Clare E. Challenger Michael Closier Tim J. Evans Mark L. Lewis John P. Mathias Carly L. Nichols Yvonne M. Po-Ba Hayley Snow Mark H. Stefaniak Hannah V. Vuong 《Tetrahedron》2006,62(29):6869-6875
The synthesis of ten substituted aminomethylene tetrahydro-isoquinolines is described, proceeding in eight steps from 5-hydroxyisoquinoline via reductive amination of N-Boc tetrahydro-isoquinoline 5-carboxaldehyde. Likewise, reductive amination was used to prepare four substituted dihydro-isoindoles from the corresponding aldehyde. The dihydro-isoindole ring system was conveniently accessed via a 2+2+2 cycloaddition reaction. 相似文献
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Liposomes with encapsulated carboxyfluorescein were used in an affinity-based assay to provide signal amplification for small-volume fluorescence measurements. Microfluidic channels were fabricated by imprinting in a plastic substrate material, poly(ethylene terephthalate glycol) (PETG), using a silicon template imprinting tool. Streptavidin was linked to the surface through biotinylated-protein for effective immobilization with minimal nonspecific adsorption of the liposome reagent. Lipids derivatized with biotin were incorporated into the liposome membrane to make the liposomes reactive for affinity assays. Specific binding of the liposomes to microchannel walls, dependence of binding on incubation time, and nonspecific adsorption of the liposome reagent were evaluated. The results of a competitive assay employing liposomes in the microchannels are presented. 相似文献
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ULTRAVIOLET ACTION SPECTRA FOR PHOTOBIOLOGICAL EFFECTS IN CULTURED HUMAN LENS EPITHELIAL CELLS 总被引:2,自引:0,他引:2
Abstract— The action spectrum for cell killing by UV radiation in human lens epithelial (HLE) cells is not known. Here we report the action spectrum in the 297–365 nm region in cultured HLE cells with an extended lifespan (HLE B-3 cells) and define their usefulness as a model system for photobiological studies. Cells were irradiated with monochromatic radiation at 297, 302, 313, 325, 334 and 365 nm. Cell survival was determined using a clonogenic assay. Analysis of survival curves showed that radiation at 297 nm was six times more effective in cell killing than 302 nm radiation; 297 nm radiation was more than 260, 590, 1400 and 3000 times as effective in cell killing as 313, 325, 334 and 365 nm radiation, respectively. The action spectrum was similar in shape to that for other human epithelial cell lines and rabbit lens epithelial cells. The effect of UV radiation on crystallin synthesis was also determined at different wavelengths. To determine whether exposure to UV radiation affects the synthesis of β-crystallin, cells were exposed to sublethal fluences of UV radiation at 302 and 313 nm, labeled with [35S]methionine and the newly synthesized βY-crystallin was analyzed by immunoprecipitation and western blotting using an antibody to β-crystallin. The results show a decrease in crystallin synthesis in HLE cells irradiated at 302 and 313 nm at fluences causing low cytotoxicity. The effect of radiation on membrane perturbation was determined by measuring enhancement of synthesis of prostaglandin E2 (PGE2). Synthesis of PGE2 occurs at all UV wavelengths tested in the 297–365 nm region. The slope of the PGE2 response curves was higher than that of cell killing curves in cultured HLE cells. These data show that cultured HLE cells with extended lifespan are a suitable system for investigating photobiological responses of cells to UV radiation. 相似文献
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B.W. Clare P.J. Jennings J.C.L. Cornish G. Talukder C.P. Lund G.T. Hefter 《Journal of computational chemistry》1993,14(12):1423-1428
A set of simple models of hydrogenated amorphous silicon (a-Si:H) consisting of hypothetical silane molecules with diamond or similar lattices was studied by the semiempirical AM1 method. Densities of states and infrared spectra were calculated for the silane molecules and similar molecules with dangling bonds disorder, and with boron or phosphorus substitution to simulate doping. Some examples are presented, and a comparison is made with experimental properties of a-Si:H. It is proposed to use these models in a study of the Staebler–Wronski photodegradation of a-Si:H and other aspects of amorphous silicon technology. © John Wiley & Sons, Inc. 相似文献
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Plunkett KN Mohraz A Haasch RT Lewis JA Moore JS 《Journal of the American Chemical Society》2005,127(42):14574-14575
The net charge of a colloidal particle was controlled using light and a new photocleavable self-assembled monolayer (SAM). The SAM contained a terminal ammonium group and a centrally located carboxylic acid group that was masked with an ortho-nitrobenzyl functionality. Once exposed to UV light, the 2-nitrobenzyl group was cleaved, therefore transforming the colloidal particle from a net positive (silica-SAM-NH3+) to a net negative (silica-SAM-COO-) charge. By varying the UV exposure time, their zeta potential could be tailored between +26 and -60 mV at neutral pH. To demonstrate a photoinduced gel-to-fluid phase transition, a binary colloidal suspension composed of silica-SAM-NH3+ and negatively charged, rhodamine-labeled silica particles was mixed to form a gel. Exposure to UV light rendered all of the particles negative and therefore converted the system into a colloidal fluid that settles to form a dense sediment. 相似文献
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19F and (119)Sn MAS NMR spectroscopy have been used to investigate the fluoride ion conductor, BaSnF(4), a member of the MSnF(4) family of fluorite-related anionic conductors containing double layers of Sn(2+) and M(2+) cations. Two fluorine sublattices were observed by (19)F MAS NMR, which could be assigned to specific sites in the lattice. The first sublattice is due to fluorine atoms located in Ba(2+) double layers and is rigid on the MAS NMR time scale at room temperature. The second sublattice comprises the fluoride ions between the Ba(2+) and Sn(2+) layers, and the few fluorine atoms that inhabit the Sn(2+)-Sn(2+) double layers. These ions are in rapid exchange with each other, and an extremely short correlation time tau(C) for the motion of these ions of <3 x 10(-)(5) s is obtained at -100 degrees C. T(1) measurements indicate that tau(C) approaches 10(-)(8) s at room temperature. (19)F-to-(119)Sn cross-polarization (CP) experiments confirmed the assignments of the resonances, and that the fluorine atoms located next to the tin atoms are extremely mobile at room temperature (and thus do not contribute to the CP process). Two-dimensional (19)F exchange experiments showed that exchange between the rigid and mobile lattice does occur, but at a much slower rate (tau(C) approximately 10 ms at 250 degrees C). Low-temperature (19)F MAS and (19)F-to-(119)Sn CP NMR spectra demonstrate that the motion of the fluoride ions has almost completely frozen out by -150 degrees C. The results are consistent with rapid two-dimensional (anisotropic) conductivity involving the fluoride ions between the Ba and Sn layers. Conductivity in three dimensions requires hops between the ions in the BaF(2)-like layers and the mobile ions. This process does occur, but with exchange rates that are at least 6-7 orders of magnitude slower. 相似文献