Discrimination of basal cell carcinoma from normal dermal stroma by quantitative multiphoton imaging

We performed multiphoton fluorescence (MF) and second-harmonic generation (SHG) imaging on human basal cell carcinoma samples. In the dermis, basal cell carcinomas can be identified by masses of autofluorescent cells with relatively large nuclei and marked peripheral palisading. In the normal dermis, SHG from dermal collagen contributes largely to the multiphoton signal. However, within the cancer stroma, SHG signals diminish and are replaced by autofluorescent signals, indicating that normal collagen structures responsible for SHG have been altered. To better delineate the cancer cells and cancer stroma from the normal dermis, a quantitative MF to SHG index is developed. We demonstrate that this index can be used to differentiate cancer cells and adjacent cancer stroma from the normal dermis. Our work shows that MF and SHG imaging can be an alternative for Mohs' surgery in the real-time guidance of the secure removal of basal cell carcinoma.     

A Continuous-Wave Electron Paramagnetic Resonance Study of Carbon Dioxide Adsorption on the Metal–Organic Frame-Work MIL-53

Continuous-wave electron paramagnetic resonance spectroscopy is applied to explore the adsorption of carbon dioxide (CO₂) over the metal organic framework (MOF) MIL-53. Therefore, paramagnetic Cr³⁺ ions, which replace a small amount of the bulk Al³⁺ ions in MIL-53(Al/Cr), are used as magnetically active probes. CO₂ was adsorbed on samples of MIL-53(Al/Cr) at equilibrium pressures between 0 and 2.5 bar. The transformation from the large pore phase to the narrow pore phase of MIL-53 was observed by electron paramagnetic resonance spectroscopy at small CO₂ pressures between 0.2 and 0.4 bar, which is in accordance with adsorption results reported in literature. By analyzing the electron paramagnetic resonance signal intensities of the corresponding Cr³⁺ probes, the ratio between the amount of the narrow pore phase and the large pore phase before and after this phase transformation was quantified. A small fraction of the large pore phase remains even after this phase transition. CO₂ adsorption at 77  K indicates the occurrence of the transformation of this MOF from a narrow pore phase to a large pore phase triggered by the adsorbed CO₂. Similar observations were already made using powder X-ray diffraction or infrared spectroscopy. But in contrast to these methods electron paramagnetic resonance spectroscopy on Cr³⁺ seems to be very sensitive not only to large differences between crystallographic conformations like large pores and narrow pores but also to different amounts and configurations of CO₂ molecules trapped in the same structural phase of MIL-53, taking advantage of the high sensitivity of the fine structure interaction of Cr³⁺.     

Chemically programmed cell adhesion with membrane-anchored oligonucleotides

Cell adhesion organizes the structures of tissues and mediates their mechanical, chemical, and electrical integration with their surroundings. Here, we describe a strategy for chemically controlling cell adhesion using membrane-anchored single-stranded DNA oligonucleotides. The reagents are pure chemical species prepared from phosphoramidites synthesized in a single chemical step from commercially available starting materials. The approach enables rapid, efficient, and tunable cell adhesion, independent of proteins or glycans, by facilitating interactions with complementary labeled surfaces or other cells. We demonstrate the utility of this approach by imaging drug-induced changes in the membrane dynamics of non-adherent human cells that are chemically immobilized on a passivated glass surface.     

Dielectric-breakdown-like forming process in the unipolar resistance switching of Ta2O5−x thin films

We report unipolar resistance switching (URS) in Ta₂O_5−x thin films. The current increased suddenly when we applied voltages up to 5-7 V to the pristine state of Pt/Ta₂O_5−x/Pt, Ni/Ta₂O_5−x/Pt, and Ti/Ta₂O_5−x/Pt cells. Just after this forming process, we observed a repetitive URS occurring independently of the electrodes. We found that the required voltages for the forming process did not depend on the top electrode type, but on the film thickness. These results suggest that the forming process is driven by a dielectric-breakdown-like phenomenon, and that URS occurs due to the formation and rupture of conducting channels inside the Ta₂O_5−x thin film.     

Thermally controlled wettability of a nanoporous membrane grafted with catechol-tethered poly(N-isopropylacrylamide)

A nanoporous membrane is coated with catechol-tethered poly(N-isopropylacrylamide). The thermosensitive variation of surface wettability determines the hindered diffusivity of dextran (40 kDa) through the nanopores.     

A new norsesterterpenoid from the sponge species <Emphasis Type="Italic">sarcotragus</Emphasis>

A new norsesterterpenoid 1 was isolated from the Sarcotragus sponge by bioactivity-guided fractionation. The structure was established on the basis of NMR and MS analysis.     

Stabilization of Enzyme by Exclusive Volume Effect in Hydrophobically Controlled Polymer Microcapsules

Summary: This communication describes an enzyme stabilization method that allows the use of enzymes irrespective of environmental factors, especially heat, while maintaining their activity for a long time. We have designed enzyme microcapsules that consist of papain enzyme cores, poly(propylene glycol) interlayers, and poly(ε‐caprolactone) walls. By confocal laser scanning microscopy measurements and the thermal stability of papain‐loaded microcapsules, it is demonstrated that the papain is surrounded by a hydrophobic polyol layer and stabilized by the exclusive volume effect. In our study, improved thermal stability can be obtained by using more hydrophobic long‐chained polyols, which is understood to be attributed to the effective formation of a hydrophobic polyol layer between the papain and the polymer wall by means of conformational anchoring in the interface.

(A) A CLSM image of a PCL microcapsule containing FITC‐labeled papain and RBITC‐labeled PPG at the same time. (B) A scheme of the role of hydrophobic polyols in the interface of enzyme and polymer.     

Machine-learning algorithms for asthma,COPD, and lung cancer risk assessment using circulating microbial extracellular vesicle data and their application to assess dietary effects

Although mounting evidence suggests that the microbiome has a tremendous influence on intractable disease, the relationship between circulating microbial extracellular vesicles (EVs) and respiratory disease remains unexplored. Here, we developed predictive diagnostic models for COPD, asthma, and lung cancer by applying machine learning to microbial EV metagenomes isolated from patient serum and coded by their accumulated taxonomic hierarchy. All models demonstrated high predictive strength with mean AUC values ranging from 0.93 to 0.99 with various important features at the genus and phylum levels. Application of the clinical models in mice showed that various foods reduced high-fat diet-associated asthma and lung cancer risk, while COPD was minimally affected. In conclusion, this study offers a novel methodology for respiratory disease prediction and highlights the utility of serum microbial EVs as data-rich features for noninvasive diagnosis.Subject terms: Respiratory tract diseases, Machine learning, Predictive markers     

积分球腔内BaTiO3：Ce晶体的自泵浦相位共轭增强现象的观测

报道了聚四氟乙烯积分球腔内光折变晶体ＢａＴｉＯ３：Ｃｅ在Ｈｅ－Ｎｅ激光作用下产生自泵浦相位共轭的增强现象，并对实验结果进行了分析。     

Trigonometric laws on Lorentzian sphere S 1 2

In this work we set up trigonometric laws and a new parallel angle formula on S ₁ ² in terms of Lorentz lengths and pseudo-angles. Thus all the laws have the same form as those of spherical trigonometry. The new parallel angle formula, however, contrasts well with that of Lobatschevsky in hyperbolic geometry.