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11.
We used the recombinant phage display antibody system (RPAS) to obtain chimeric single-chain fragment variable (ScFv) antibodies
to gill proteins of the white clam Codakia orbicularis (Linné, 1758). After three rounds of selection on immunotubes loaded with total gill protein extract, recombinant phages
exhibiting antibodies to gill proteins were isolated and tested by enzyme-linked immunosorbent assay (ELISA). Clones exhibiting
a high affinity for the mollusk proteins were selected for production of soluble ScFv antibodies, which were purified for
subsequent analysis. ScFv antibodies exhibited a reaction specific for a protein whose molecular mass was about 15,000 Daltons
and that was detected by the antigen capture technique followed by sodium dodecyl sulfate polyacrylamide gel electrophoresis
and Western blotting. 相似文献
12.
Timoshenko VM Bouillon JP Chernega AN Shermolovich YG Portella C 《Chemistry (Weinheim an der Bergstrasse, Germany)》2003,9(18):4324-4329
The ethyl ester of beta-bromoperfluorodithiocrotonic acid reacts with dimethyl acetylenedicarboxylate to give 1,4-difluoro-2,3-bis(trifluoromethyl)-but-2-ene-1,4-diylidene-2,2'-bis(4',5'-dicarbomethoxy-1',3'-dithiole) (4), a new type of vinylogue of tetrathiafulvalene. The thermal transformations of this compound lead, depending on the temperature, to the formation of the cyclization products: 11,14-difluoro-2,3,8,9-tetra(carbomethoxy)-12,13-bis(trifluoromethyl)-1,4,7,10-tetrathiadispiro[4.0.4.4]tetradeca-2,8,11,13-tetraene (8) or 5,8-difluoro-6,7-bis(trifluoromethyl)-2,3-bis(carboxymethyl)-1,4-benzodithiine (11). 相似文献
13.
Marcio Gameiro Jean-Philippe Lessard Alessandro Pugliese 《Foundations of Computational Mathematics》2016,16(2):531-575
In this paper, we introduce a constructive rigorous numerical method to compute smooth manifolds implicitly defined by infinite-dimensional nonlinear operators. We compute a simplicial triangulation of the manifold using a multi-parameter continuation method on a finite-dimensional projection. The triangulation is then used to construct local charts and an atlas of the manifold in the infinite-dimensional domain of the operator. The idea behind the construction of the smooth charts is to use the radii polynomial approach to verify the hypotheses of the uniform contraction principle over a simplex. The construction of the manifold is globalized by proving smoothness along the edge of adjacent simplices. We apply the method to compute portions of a two-dimensional manifold of equilibria of the Cahn–Hilliard equation. 相似文献
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15.
Solène Morand Prof. Philippe Jubault Prof. Jean-Philippe Bouillon Dr. Samuel Couve-Bonnaire 《Chemistry (Weinheim an der Bergstrasse, Germany)》2021,27(69):17273-17292
gem-Heteroatom-substituted fluoroalkenes have received little attention despite their great potential in medicinal chemistry or in fine chemistry. Indeed, due to the electronic and steric similarity between the fluoroalkene moiety and the amide bond as well as the high strength of the carbon-fluorine bond, these gem-heteroatom-substituted fluoroalkenes could be envisioned as stable mimics of various important organic functions, such as phosphates, carbamates, S-thiocarbamates and ureas. We present herein an overview describing the syntheses over the last decade of heteroatom-substituted fluoroalkenes in geminal position. This review will be divided into several sections covering each the common following heteroatom: oxygen-, nitrogen-, sulfur-, phosphorus-, boron- and silicon-substituted fluoroalkenes. 相似文献
16.
Pablo López-Tarifa Marie-Anne Hervé du Penhoat Rodophe Vuilleumier Marie-Pierre Gaigeot Ursula Rothlisberger Ivano Tavernelli Arnaud Le Padellec Jean-Philippe Champeaux Manuel Alcamí Patrick Moretto-Capelle Fernando Martín Marie-Françoise Politis 《Central European Journal of Physics》2014,12(2):97-102
We use time-dependent density functional theory and Born-Oppenheimer molecular dynamics methods to investigate the fragmentation of doubly ionized uracil in gas phase. Different initial electronic excited states of the dication are obtained by removing electrons from different inner-shell orbitals of the neutral species. We show that shape-equivalent orbitals lead to very different fragmentation patterns revealing the importance of the intramolecular chemical environment. The results are in good agreement with ionion coincidence measurements of uracil collision with 100 keV protons. 相似文献
17.
We report the operation of a 50 mW continuous-wave quantum cascade laser (QCL) in the 9.2 microm range, phase locked to a single-mode CO(2) laser with a tunable frequency offset. The wide free-running emission spectrum of the QCL (3-5 MHz) is strongly narrowed down to the kilohertz range, making it suitable for high-resolution molecular spectroscopy. 相似文献
18.
Jean-Philippe Uzan George F. R. Ellis Julien Larena 《General Relativity and Gravitation》2011,43(1):191-205
In order to understand how locally static configurations around gravitationally bound bodies can be embedded in an expanding
universe, we investigate the solutions of general relativity describing a space–time whose spatial sections have the topology
of a 3-sphere with two identical masses at the poles. We show that Israel junction conditions imply that two spherically symmetric
static regions around the masses cannot be glued together. If one is interested in an exterior solution, this prevents the
geometry around the masses to be of the Schwarzschild type and leads to the introduction of a cosmological constant. The study
of the extension of the Kottler space–time shows that there exists a non-static solution consisting of two static regions
surrounding the masses that match a Kantowski–Sachs expanding region on the cosmological horizon. The comparison with a Swiss-Cheese
construction is also discussed. 相似文献
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