Preliminary report on detection of papillomaviruses types 6, 11, 16, and 18 in laryngeal benign and malignant lesions

Recently, clinicians have observed an increase in the incidence of laryngeal papilloma coincident with the rising incidence of venereal warts. The lesions occur in young adults of professional singing age. Different types of human papillomavirus (HPV) are presented. Gynecologic exam revealed cervical tumors, involving 18 HPV in benign and malignant lesions. Thirty patients with the following pathologic conditions were studied: 10 papillomas, 10 severe dysplasias, and 10 invasive cancers. All the lesions were located on the free edge of the vocal folds or the anterior commissure involving the epithelium. The results are as follows: 11 patients (33%) had virus infection, of which four had HPV 6 or HPV 11 on laryngeal papilloma, three had HPV 16 or HPV 18 on severe dysplasia, four had HPV 16 or HPV 18 on carcinoma; and 19 (66%) had no virus infection. Gynecologic research in this field is more advanced. However, this preliminary report raises interesting research questions in laryngology.     

Hétérocycles contenant du phosphore. XXXII. Etude de l'action des phosphorhydrazides sur les orthoesters,l'anhydride acétique et l'acide formique

The reaction of phosphodi- or trihydrazides with orthoesters affords mainly 2,3,4,5-tetrahydro-1,2,4,5,3-tetrazaphosphorines and sometimes 4-amino-1,2,4,3-triazaphospholidines. These 1,2,4,5,3-tetrazaphosphorines present a prototropy which explains different pathways of the reaction of these derivatives with formaldehyde or acetone, The structure of all heterocycles which were obtained was established by nmr spectra.     

Emissionsspektroskopie mit induktiv gekoppelter Plasmaflamme als Detektor zur Elementanalyse nach gas-chromatographischer Trennung

Zusammenfassung Die Kombination eines Gas-Chromatographen mit dem Stickstoff/Argon-Plasma (ICPOES) bei hoher Leistung wird beschrieben und mit Pb- und Si-haltigen organischen Verbindungen getestet. Die direkte Anpassung von GC-Ausgang und Gaseinlaß am ICP-Brennersystem sowie das Aufheizen der Verbindung und des Trägergases ermöglichen die Eingabe kleiner Gasmengen in das Plasma. Die Ergebnisse dieser ersten Studien lassen erkennen, daß sich die ICPOES zur Lösung zahlreicher Probleme im Bereich der Spurenanalyse, der Anreicherung und gas-chromatographischen Trennung organischer Metallkomplexe wird einsetzen lassen, wenn die ICP-Anregung als Detektor in Verbindung mit einem Simultanspektrometer eingesetzt wird. Die Empfindlichkeit des N₂-/Ar-Plasmas bei hoher Leistung wird mit derjenigen des Ar-Plasmas bei niedriger Leistung verglichen. Als analytisches Beispiel wird die Bestimmung geringer Pb-Gehalte in Benzinen beschrieben, wobei auch das Verhältnis Bleitetraethyl/-tetramethyl bekannt wird.

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ICP optical emission spectrometry as a detector for elemental analysis after gas-chromatographic separation

Summary The combination of a gas-chromatographic device with high-power nitrogen/argon inductively coupled plasma optical emission spectrometry (ICPOES) is described and tested with Pb and Si containing organic compounds. The direct combination of the GC output with the input tube of the ICP burner system and a heating-up device of the carrier gas make it possible to introduce small gas amounts into the ICP torch. The results of these studies suggest that ICP-OES should be applicable to solve many problems of trace elemental analysis, preconcentration and GC separation of organic metal complexes by using the ICP source as a detector system in connection with the simultaneous OES. The sensitivity of high-power nitrogen/ argon ICP is compared with that of low-power argon ICP. For example the determination of low contents of lead in petrol or fuels is given in detail. The ratio of lead tetraethyl/tetramethyl can be determined, too.